Most antibodies from Cell Signaling Technology (CST) are provided in a standard 100 µl (small) / 300 µl (large) volume. Generally, our antibodies are formulated based on the number of western blots that can be performed at the recommended dilution by following CST protocol. If the antibody is not recommended for western blotting, the number of assays will refer to the recommended application.
We have defined the following volumes per assay to establish the number of assays provided per vial:
|ChIP||500 µl chromatin in 1X ChIP buffer|
|Flow Cytometry||100 µl antibody diluent|
|Immunofluorescence||100 µl antibody diluent|
|Immunohistochemistry||100 µl antibody diluent|
|Immunoprecipitation||200 µl cell lysate in 1X cell lysis buffer|
|Western blotting||10 ml antibody diluent|