Cell Signaling Technology

Immunohistochemistry Technical Support

NOTE: For the best possible results, Cell Signaling Technology (CST) strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at CST and ensure accurate and reproducible results.

To ensure a timely solution, please have the information below ready when you contact us. Technical Support can be reached by toll free phone at 877-678-8324 or 877-678-TECH, fax at 978-867-2400 or e-mail at support@cellsignal.com.

Tissue

  1. Species.
  2. Organ.
  3. Fixation (drop fixed, perfused, or unfixed).
    1. Fixative used
    2. Time
    3. Temperature
  4. Sections (paraffin-embedded, fixed cryosections, fresh frozen, floating).

Antigen Retrieval

  1. Buffer.
  2. Describe procedure (time, temperatures, apparatus, etc.).

Hydrogen Peroxide Quench Performed?

  1. Buffer.
  2. Time.

Block

  1. Buffer.
  2. Time.
  3. Temperature.

Primary Antibody

  1. Catalog number.
  2. Lot number and reference date.
  3. Dilution.
  4. Diluent.
  5. Length of incubation.
  6. Temperature of incubation.

Wash Buffer

Secondary Reagents

  1. Manufacturer.
  2. Dilution.
  3. Time.
  4. Temperature.

Substrate

  1. Name.
  2. Length of incubation.

Results

  1. Localization/background.

Controls

  1. Negative used and result.
  2. Positive used and result.

Support