Cell Signaling Technology

Alexa Fluor® Conjugates

These antibodies are conjugated to Alexa Fluor® 488, 555 and 647, and are useful in in flow cytometry and immunofluorescence (IF-IC, IF-F) applications. The combination of Alexa Fluor® dyes with superior brightness and photostability with the highest quality antibodies from Cell Signaling Technology results in the brightest signal with the lowest background. All Alexa Fluor® Conjugates are validated in-house for the specified applications.

New More Concentrated Formulation

In order to improve application flexibility, Cell Signaling Technology has moved to a standardized, more concentrated formulation of 100 μl, for all dye conjugated primary antibodies. This new formulation still provides at least 50 tests per vial, but now allows for greater flexibility when performing multiplexing immunofluorescence or flow cytometry assays.

Companion Products

No. Isotype Control Alexa 488 Alexa 555 Alexa 647
Mouse IgG1 Isotype Control (MOPC-21) 4878 4843
3900 Rabbit (DA1E) mAb IgG XP™ Isotype Control 2975 2985
Rabbit IgG (Polyclonal) Isotype Control 4340 3452

488, 555 & 647 Conjugates

No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 647
No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 647
2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb H, M, R, Mk, Dm 2846 5123
2965 Phospho-Akt (Thr308) (C31E5E) Rabbit mAb H, M, R, Mk, Hm 2918 3375
4058 Phospho-Akt (Ser473) (193H12) Rabbit mAb H, M, R 2336 2337
4060 Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb H, M, R, Mk, Hm, B, Dm, Z, (Pg, C, X, Dg) 4071 4075
2966 Akt (5G3) Mouse mAb H, M, R, Hm 2917 2944
2827 Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb H 2834
2764 Bcl-xL (54H6) Rabbit mAb H, M, R, Mk 2767
3195 E-Cadherin (24E10) Rabbit mAb H, M, (Dg) 3199 4295
9661 Cleaved Caspase-3 (Asp175) Antibody H, M, R, Mk, B, (Pg) 9669
2677 β-Catenin (L54E2) Mouse mAb (IF Preferred) H, (M, R, Mk, Pg) 2849 4627
3570 CD44 (156-3C11) Mouse mAb H 3516
4850 COX IV (3E11) Rabbit mAb H, R, Mk, Pg, B, Z 4853
9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb H, M, R 9187
4135 Cyclin B1 (V152) Mouse mAb H, M, (Hm) 4112 4118
2368 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) All 5407 3768 3916
2251 FosB (5G4) Rabbit mAb H, M, R 2023
2118 GAPDH (14C10) Rabbit mAb H, M, R, Mk 3906 3964 3907
3670 GFAP (GA5) Mouse mAb H, M, R 3655 3656 3657
2367 HA-Tag (6E2) Mouse mAb All 2350 3444
2024 Hexokinase I (C35C4) Rabbit mAb H, M 3689 3540
9718 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb H, M, R, Mk 9719 9720
3377 Phospho-Histone H3 (Ser10) (D2C8) XP™ Rabbit mAb H, M, R, Mk, Z 3465 3475 3458
9701 Phospho-Histone H3 (Ser10) Antibody H, M, R, Mk, Sc, C, Dm, Z, (X) 9708 9716
9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb H, M, Z, (R, Mk) 9683
4876 HSP70 (D69) Antibody H, M, R, Mk 4837 4008
2624 GST (26H1) Mouse mAb All 3368 3720 3445
4545 Pan-Keratin (C11) Mouse mAb H, R, Mk 4523 4528
3308 c-Kit (Ab81) Mouse mAb H 3310
4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP™ Rabbit mAb H, M, R, Mk, Mi, Pg, Sc, Hm, B, Dm, Z, Dg, (Ce) 4344 4284
9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb H, M, R, Mk, Mi, Pg, Hm, B, Dm, Z 4374 4375
4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb H, M, R, Mk, Mi, Pg, Sc, Hm, B, Dm, Z, Ce, (C, Dg) 4780 5376
3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb H, M, R, Mk 4338 4320
9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb H, M, R, Mk, Sc, Dm, (Z) 4551 4552
9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb H, M, R, Mk, Dm, (C) 4660 5068
2276 Myc-Tag (9B11) Mouse mAb All 2279 3756 2233
3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb H, M, R, Mk, Pg, Hm, (Dg) 4886 4887
2840 Oct-4A (C30A3) Rabbit mAb H, M 5177 5263
9286 Phospho-p53 (Ser15) (16G8) Mouse mAb H 9235
2524 p53 (1C12) Mouse mAb H, M, R, Mk 2015 2533
9559 PTEN (138G6) Rabbit mAb H, M, R, Mk 9560 9573
2258 PU.1 (9G7) Rabbit mAb H, M, (Mk, Pg) 2216 2240
4856 Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb H, M, R 4854
4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb H, M, R, Mk, Sc 4803 3985 4851
9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb H, M, R, Sc, Hm 9257
9167 Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb H, M 9174
9145 Phospho-Stat3 (Tyr705) (D3A7) XP™ Rabbit mAb H, M, R, Mk 4323 4324
9314 Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb H, M, (R, Mk, B) 3939 9365
2808 Survivin (71G4B7) Rabbit mAb H, M, R 2810 2866
2128 β-Tubulin (9F3) Rabbit mAb H, M, R, Mk, B, Dm, Z, (C) 3623 2116 3624
9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100) All 9414 9415
2479 VEGF Receptor 2 (55B11) Rabbit mAb H, M 3627 3628
Zap-70 2707

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

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