Cell Signaling Technology

Alexa Fluor® Conjugates

These antibodies are conjugated to Alexa Fluor® 488, 555, 594 and 647, and are useful in in flow cytometry and immunofluorescence (IF-IC, IF-F) applications. The combination of Alexa Fluor® dyes with superior brightness and photostability with the highest quality antibodies from Cell Signaling Technology results in the brightest signal with the lowest background. All Alexa Fluor® Conjugates are validated in-house for the specified applications.

Cellular Dyes

No. Unconjugated Antibody Alexa 488 Alexa 555 Alexa 594 Alexa 647
Phalloidin 8878 8953 8940

Companion Products

No. Unconjugated Antibody Alexa 488 Alexa 555 Alexa 594 Alexa 647
Mouse IgG1 Isotype Control (MOPC-21) 4878 4843
5415 Mouse (G3A1) mAb IgG1 Isotype Control 9641 8527
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control 2975 3969 8760 2985
Rabbit IgG (Polyclonal) Isotype Control 4340 3452

Epitope Tag Antibodies

No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 594 Alexa 647
2368 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) All 5407 3768 9696 3916
2367 HA-Tag (6E2) Mouse mAb All 2350 3444
2624 GST (26H1) Mouse mAb All 3368 3720 9440 3445
2276 Myc-Tag (9B11) Mouse mAb All 2279 3756 9483 2233

Target-specific Antibodies

No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 594 Alexa 647
No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 594 Alexa 647
2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb H, M, R, Mk, Dm 2846 5123
4970 β-Actin (13E5) Rabbit mAb H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr) 8844 8046 9470 8584
3903 AFP (3H8) Mouse mAb H, M 7741 7800 7877 7765
2965 Phospho-Akt (Thr308) (C31E5E) Rabbit mAb H, M, R, Hm, Mk 2918 3375
4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg) 4071 4075
4058 Phospho-Akt (Ser473) (193H12) Rabbit mAb H, M, R 2336 2337
4691 Akt (pan) (C67E7) Rabbit mAb H, M, R, Mk, Dm, (Pg) 5084 5186
2966 Akt (5G3) Mouse mAb H, M, R, Hm 2917 2944
5153 Androgen Receptor (D6F11) XP® Rabbit mAb H 7395 8956 8938 7397
2914 Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb H, M, R 8525
2827 Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb H 2834
2764 Bcl-xL (54H6) Rabbit mAb H, M, R, Mk 2767
3195 E-Cadherin (24E10) Rabbit mAb H, M, (B, Dg, Pg) 3199 4295 7687 9835
9661 Cleaved Caspase-3 (Asp175) Antibody H, M, R, Mk, (B, Dg, Pg) 9669
9579 Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb H, (M, R, Mk, B, Pg) 9603 8172 9602
2677 β-Catenin (L54E2) Mouse mAb (IF Preferred) H, (M, R, Mk, Pg) 2849 5612 4627
3570 CD44 (156-3C11) Mouse mAb H 3516
5664 CNPase (D83E10) XP® Rabbit mAb H, M, R 5714 5715 5716
4850 COX IV (3E11) Rabbit mAb H, R, Mk, Z, B, Pg 4853 8693 8692 7561
9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb H, M, R, (Z) 9187
4135 Cyclin B1 (V152) Mouse mAb H, M, (Hm) 4112 4118
2306 DARPP-32 (19A3) Rabbit mAb M, R, (H) 11893
4267 EGF Receptor (D38B1) XP® Rabbit mAb H, M, Mk 5616 5108 8742 5588
2929 EpCAM (VU1D9) Mouse mAb H 5198 5488 7319 5447
5348 Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb H, M, R, (Hm, Mk, B, Pg, Hr) 8709 8677
2251 FosB (5G4) Rabbit mAb H, M, R 2023
2118 GAPDH (14C10) Rabbit mAb H, M, R, Mk, (Pg) 3906 3964 3907
3670 GFAP (GA5) Mouse mAb H, M, R 3655 3656 8152 3657
3660 Glucocorticoid Receptor (D8H2) XP® Rabbit mAb H, M, R, Mk 12007
2024 Hexokinase I (C35C4) Rabbit mAb H, M 3689
9718 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb H, M, R, Mk 9719 8228 9720
3377 Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb H, M, R, Mk, Z 3465 3475 8481 3458
9701 Phospho-Histone H3 (Ser10) Antibody H, M, R, Mk, C, Dm, Z, Sc, (X) 9708 9716
9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb H, M, R, Mk, Z, Ce, (Sc) 9683 5489 8823 4484
4473 Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb H, M, R, Mk, (C, Dm, X, Z, B, Pg, Sc, Ce) 12086
9733 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb H, M, R, Mk, (X, Z) 5499 12158
9728 Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb H, M, R, Mk 12244
9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb H, M, R, Mk, Dm, Sc, (X, Z) 12064
3890 HS1 (D83A8) XP® Rabbit mAb (Human Specific) H 12162
4876 HSP70 (D69) Antibody H, M, R, Mk 4837 4008
4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) H, M, R, Mk, B, Pg, GP 5743 8993
3014 Insulin (C27C9) Rabbit mAb H, M, R 9016 9008
4543 Keratin 17 (D73C7) XP® Rabbit mAb H, M, R, (Mk, Dg) 8856
4545 Pan-Keratin (C11) Mouse mAb H, R, Mk 4523 3478 4528
9129 Ki-67 (D3B5) Rabbit mAb H, M, R 11882
3308 c-Kit (Ab81) Mouse mAb H 3310
4777 Lamin A/C (4C11) Mouse mAb H, M, R, Mk 8617
2230 LEF1 (C12A5) Rabbit mAb H, M, R 8490
8641 LIN28A (D1A1A) XP® Rabbit mAb H, M, (R, Mk) 11845
4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Sc, (C, Ce) 4344 4284
9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb H, M, R, Hm, Mk, Mi, Dm, Z, B, Pg 4374 4375
4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Ce, (C) 4780 5376
3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb H, M, R, Mk 4338 4320
4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb H, M, R, Mk, Mi, Pg, Sc, (Hm, C, Z, B) 9836 8632
9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb H, M, R, Mk, Sc, (Z) 4551 4552
3077 Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb H, M, R 8564
8198 Met (D1C2) XP® Rabbit mAb H 8494
3329 MNDA (3C1) Rat mAb H 9283
2983 mTOR (7C10) Rabbit mAb H, M, R, Mk, (Hr) 5043
4903 Nanog (D73G4) XP® Rabbit mAb H, (Mk) 8750 5448
5482 Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb H, M, R, Mk 6992 9851 8453 7497
4760 Nestin (Rat-401) Mouse mAb R 6994 8181 8484
3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb H, M, R, Hm, Mk, Pg, (Dg) 4886 4887
2837 Neurofilament-L (C28E10) Rabbit mAb H, M, R 8024 8039 8743 8590
2840 Oct-4A (C30A3) Rabbit mAb H, M 5177 4439 5263
2947 p21 Waf1/Cip1 (12D1) Rabbit mAb H, Mk 5487 8493 11850 8587
9286 Phospho-p53 (Ser15) (16G8) Mouse mAb H 9235 9481
2527 p53 (7F5) Rabbit mAb H, Mk 5429 5395
2524 p53 (1C12) Mouse mAb H, M, R, Mk 2015 2533
5625 Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb H, Mk 9148 6894 6987
8970 PAX5 (D19F8) XP® Rabbit mAb H, M, (X) 11849 11852
2586 PCNA (PC10) Mouse mAb H, M, R, Mk, B, Pg 8580
5241 PDGF Receptor α (D13C6) XP® Rabbit mAb H 8871 8893 9015 5876
3501 PDI (C81H6) Rabbit mAb H, M, R, Mk 5051 8448 8615 8463
2258 PU.1 (9G7) Rabbit mAb H, M, (Mk, Pg) 2216 2240
4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb H, M, R, Mk, Mi, Sc, (C, Pg) 4803 3985 9865 4851
8516 Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb H, M, R, Mk 4277 8957 8880 8974
4856 Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb H, M, R, Mk 4854
5364 Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb H, M, R, Mk, (C, Pg) 5018 9468 5044
2317 S6 Ribosomal Protein (54D2) Mouse mAb H, M, R, Mk, Dm 5317 6989 5548
9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb H, M, R, Hm, Sc 9257
3579 Sox2 (D6D9) XP® Rabbit mAb H, (Mk, B, Dg, Hr) 5049 5179 7685 5067
SQSTM1/p62 (D1D9E3) 8833
4755 SSEA4 (MC813) Mouse mAb H 5835 5836
9167 Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb H, M 9174 8183 8009
9145 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb H, M, R, Mk, (Hm, B, Pg, Hr) 4323 4324
9314 Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb H, M, (R, Mk, B) 3939 9365
2808 Survivin (71G4B7) Rabbit mAb H, M, R 2810 4580 2866
2710 Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb H, (M, R) 12081
4179 α-Synuclein (D37A6) XP® Rabbit mAb M, R 5496 4639
2125 α-Tubulin (11H10) Rabbit mAb H, M, R, Mk, Dm, B, (Dg, Pg) 5063 5059 5046
2128 β-Tubulin (9F3) Rabbit mAb H, M, R, Mk, Z, B, (C) 3623 2116 7634 3624
3873 α-Tubulin (DM1A) Mouse mAb H, M, R, Mk 8058
9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100) All 9414 9415
5741 Vimentin (D21H3) XP® Rabbit mAb H, M, R, Mk 9854 9855 7675 9856
3165 Zap-70 (D1C10E) XP® Rabbit mAb H, M 9473
Zap-70 2707

Reactivity Key: H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Mi=Mink  Dm=D.melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Sc=S.cerevisiae  GP=Guinea Pig  Hr=Horse  Ce=C.elegans  All=All Species Expected 
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

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