Cell Signaling Technology

Chromatin Immunoprecipitation (ChIP) Kits and Antibodies

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. Cell Signaling Technology® (CST) now has extended its expertise in antibody validation and is providing you with antibodies, kits and reagents for chromatin immunoprecipitation (ChIP).

CST’s new ChIP recommended antibodies have been validated in house by our antibody development scientists by the same rigorous standards as all other recommended applications. Technical support will be provided by the same scientists that validated the antibody, which will guarantee a fast, thorough and accurate response.

ChIP helix

SimpleChIP® Product Line

ChIP Companion Products

ChIP Validated Antibodies and Primer Pairs

No. Antibody Reactivity Positive Control
Primers		 Negative Control
Primers
No. Antibody Reactivity Positive Control
Primers		 Negative Control
Primers
9814 Acetylated-Lysine (Ac-K2-100) Rabbit mAb all species expected 4471 H, 5516 H 4486 H, 4493 H
9441 Acetylated-Lysine Antibody all species expected 5516 H, 7014 H, 7015 M 4490 H, 4493 H
6964 Bmi1 (D20B7) XP® Rabbit mAb H, Mk 7707 H, 5517 H 4486 H
5856 Bmi1 (D42B3) Rabbit mAb H, M, R, Mk 7707 H, 5517 H 4486 H
9562 β-Catenin Antibody H, M, R, Mk, (Z) 5111 H 4486 H
9581 β-Catenin Antibody (Amino-terminal Antigen) H, M, R, Mk 5111 H 4486 H
9587 β-Catenin Antibody (Carboxy-terminal Antigen) H, M, R, Mk, (C, X, B, Dg, Pg, Hr) 5111 H 4486 H
4771 Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody H, M, R, Mk 4829 H 4486 H
7389 CBP (D6C5) Rabbit mAb H, M, R, Mk 4829 H 4486 H
7425 CBP (D9B6) Rabbit mAb H, M, R, Mk 4829 H 4486 H
9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb H, M, R 4829 H 4486 H
4276 Phospho-CREB (Ser133) (D1G6) Rabbit mAb H, M, R 4829 H 4486 H
9197 CREB (48H2) Rabbit mAb H, M, R, Mk, Dm 4829 H 4486 H
4820 CREB (D76D11) Rabbit mAb H, M, R, Hm, Mk, Dm 4829 H 4486 H
3417 CTCF (D1A7) XP® Rabbit mAb H, R, Mk, (B) 5172 H 4486 H
3418 CTCF (D31H2) XP® Rabbit mAb H, M, R, Mk, (B, Hr) 5172 H 4486 H
2899 CTCF Antibody H, M, R, Mk 5172 H 4486 H
3742 E2F-1 Antibody H, M, (R) 7001 H 4486 H
4153 EGR1 (15F7) Rabbit mAb H, M, R, (B) 5549 H 4486 H
4154 EGR1 (44D5) Rabbit mAb H, M, R, (B) 5549 H 4486 H
5246 Ezh2 (D2C9) XP® Rabbit mAb H, M, R, Mk 7707 H, 5517 H 4486 H
4905 Ezh2 Antibody H, M, R, Pg 7707 H, 5517 H 4486 H
8240 Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb H, M, R, Mk 7707 H, 5517 H 4486 H, 5516 H
5546 Ubiquityl-Histone H2B (Lys120) (D11) XP® Rabbit mAb H, M, R, Mk 5047 H, 4478 H 5037 H, 4471 H
5326 Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb H, M, R, Mk 5047 H 5037 H
9725 Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb H, M, R, Mk 7014 H, 7015 M 4486 H, 4490 H
9726 Di-Methyl-Histone H3 (Lys4) Antibody H, M, R, Mk, (X, Z) 7014 H, 7015 M 4486 H, 4490 H
4473 Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb H, M, R, Mk, (C, Dm, X, Z, B, Pg, Sc, Ce) 5098 H, 4486 H, 5077 H 4471 H, 7014 H
4069 Pan-Methyl-Histone H3 (Lys9) Antibody H, M, R, Mk, Z 5098 H, 4486 H, 5077 H 4471 H, 7014 H
8173 Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb H, M, R, Mk, (Hm, X, Z, GP, Hr) 5516 H, 7014 H, 7015 M 5098 H, 4486 H
9733 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb H, M, R, Mk, (X, Z) 4490 H, 4493 H 7014 H
4353 Acetyl-Histone H3 (Lys27) Antibody H, M, R, Mk, (Hm, C, Dm, X, Z, B) 5516 H, 7014 H, 7015 M 5098 H, 4486 H
9756 Tri-Methyl-Histone H3 (Lys27) Antibody H, M, R, Mk, (X) 4490 H, 4493 H 5516 H, 7014 H
9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb H, M, R, Mk, Z, (Sc) 5516 H, 7014 H, 7015 M 4486 H, 4490 H
9671 Acetyl-Histone H3 (Lys9) Antibody H, M, R, Mk, Dm, Sc 5516 H, 7014 H, 7015 M 5098 H, 4486 H
9677 Acetyl-Histone H3 (Lys9/Lys14) Antibody H, M, R, Mk, (Z) 7014 H, 7015 M 4486 H, 4490 H
7627 Acetyl-Histone H3 (Lys14) (D4B9) Rabbit mAb H, M, R, Mk, (Hm, Dm, X, Z, Pg, Sc, Hr) 7014 H, 7015 M 4486 H, 4490 H
5275 Acetyl-Histone H3 (Lys14) Antibody (ChIP Formulated) H, (M, R, Mk) 4478 H, 7014 H, 7015 M 4486 H, 4490 H
9675 Acetyl-Histone H3 (Lys18) Antibody H, M, R 5516 H, 7014 H, 7015 M 4486 H, 4490 H
4658 Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb H, M, R, Mk, (Dm, X, Z, B, Pg, Sc, Ce) 5098 H, 4486 H 4471 H, 7014 H
9753 Di-Methyl-Histone H3 (Lys9) Antibody H, M, R, Mk, Dm, Sc 5098 H, 4486 H 5516 H, 7014 H
9728 Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb H, M, R, Mk 5098 H, 4490 H 5516 H, 7014 H
5427 Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb H, M, R, Mk 5047 H 5037 H
9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb H, M, R, Mk, Dm, Sc, (X, Z) 5516 H, 7014 H, 7015 M 4486 H, 4490 H
9727 Tri-Methyl-Histone H3 (Lys4) Antibody H, M, R, Mk, (X, Z) 5516 H, 7014 H, 7015 M 4486 H, 4490 H
5327 Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb H, M, R, Mk 5098 H, 4486 H 5516 H
4909 Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb H, M, R, Mk, (Hm, C, Dm, X, Z, B) 5047 H, 4478 H 5037 H, 4471 H
4620 Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) H, M, (R, Hm, Mk, C, Dm, X, Z, B) 4486 H, 7014 H, 7015 M
2650 Histone H3 Antibody (ChIP Formulated) H, M, (R, Mk, C, Dm, X, Z, B) 4486 H, 7014 H, 7015 M
9672 Acetyl-Histone H4 (Lys5) Antibody H, M, R, Mk, (C, Dm, X, Z, B, Pg, Hr, Ce) 7014 H 4486 H
2594 Acetyl-Histone H4 (Lys8) Antibody H, M, R, Mk, (Ce) 7014 H, 7015 M 5098 H, 4486 H
5737 Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb H, M, R, Mk, (X, B, Pg) 5098 H, 4486 H 5516 H
2960 Histone H4 (L64C1) Mouse mAb (ChIP Formulated) H, (M, R, Mk, Dm, X, Z, B, Hr, Ce) 4486 H, 4490 H
4356 HSF1 Antibody H, M, R, Mk 5551 H 4486 H
4299 IRF-4 (D43H10) Rabbit mAb H, R 5156 H 4486 H
4964 IRF-4 Antibody H 5156 H 4486 H
5628 IRF-8 (D20D8) Rabbit mAb H, M, (R, Mk, X, B) 4486 H
3619 MCM2 (D7G11) XP® Rabbit mAb H, M, R, Mk 5148 H 4486 H
5415 Mouse (G3A1) mAb IgG1 Isotype Control
5605 c-Myc (D84C12) XP® Rabbit mAb H, M, R, (Mk, Dg, Pg) 4779 H 4486 H
9402 c-Myc Antibody H, M, R, Pg 4779 H 4486 H
5232 Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) H 4641 H, 4649 H 4486 H
3580 Nanog Antibody H 4641 H, 4649 H 4486 H
4373 NeuroD (D35G2) Rabbit mAb H, M, R 5549 H 4486 H
3035 NF-κB1 p105/p50 Antibody H, Mk 5552 H 4486 H
8242 NF-κB p65 (D14E12) XP® Rabbit mAb H, M, R, Hm, Mk, Dg 5552 H 4486 H
6956 NF-κB p65 (L8F6) Mouse mAb H, M, R, Hm, Mk, Mi, B, Dg, Pg 5552 H 4486 H
5677 Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) H, M 4641 H, 4653 M, 4649 H, 4659 M 4486 H, 7015 M
2890 Oct-4A (C52G3) Rabbit mAb H 4641 H, 4649 H 4486 H
2750 Oct-4 Antibody H, (Mk) 4641 H, 4649 H 4486 H
9284 Phospho-p53 (Ser15) Antibody H, M, R, Mk 6449 H 4486 H
2527 p53 (7F5) Rabbit mAb H, Mk 6449 H 4486 H
2524 p53 (1C12) Mouse mAb H, M, R, Mk 6449 H 4486 H
9282 p53 Antibody H, R, Mk 6449 H 4486 H
3378 PCAF (C14G9) Rabbit mAb H, M, R, Mk, (B, Hr) 4829 H 4486 H
2629 Rpb1 CTD (4H8) Mouse mAb H, M, R, Mk, (Hm, Dm, Sc) 4471 H, 5516 H 4490 H, 4493 H
2258 PU.1 (9G7) Rabbit mAb H, M, (Mk, Pg) 4486 H
2266 PU.1 Antibody H, M, (Mk, Pg) 4486 H
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control
2729 Normal Rabbit IgG
9313 Rb (D20) Rabbit mAb H, M, Mk 7001 H 4486 H
9309 Rb (4H1) Mouse mAb H, Mk, B, Pg 7001 H 4486 H
5694 RING1B (D22F2) XP® Rabbit mAb H, M, R, Mk 7707 H, 5517 H 4486 H
6944 Smad1 (D59D7) XP® Rabbit mAb H, M, (Mk, X, B) 5139 H 4486 H
9743 Smad1 Antibody H, M, Mk 5139 H 4486 H
9511 Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody H, M, R, Mi, X 5139 H 4486 H
5339 Smad2 (D43B4) XP® Rabbit mAb H, M, R, Mk 5139 H, 4669 H 4486 H
8685 Smad2/3 (D7G7) XP® Rabbit mAb H, M, R, Mk 5139 H, 4669 H 4486 H
5678 Smad2/3 Antibody H, M, R, Mk, (X) 5139 H, 4669 H 4486 H
9520 Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb H, M, R, (Mk, X, Z, B) 5139 H, 4669 H 4486 H
9523 Smad3 (C67H9) Rabbit mAb H, M, R, Mk, (X, Z, B) 5139 H, 4669 H 4486 H
9515 Smad4 Antibody H, M, R, Mk 5139 H, 4669 H 4486 H
5024 Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated) H, (Mk, B, Dg, Hr) 4641 H, 4649 H 4486 H
2748 Sox2 Antibody H, M, (R, Mk, B, Dg, Hr) 4641 H, 4653 M, 4649 H, 4659 M 4486 H, 7015 M
5147 SRF (D71A9) XP® Rabbit mAb H, M, R, Mk, Pg 4663 H 4486 H
9167 Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb H, M 5148 H 4486 H
7649 Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb H, M, R, (Mk) 4486 H, 5148 H
9171 Phospho-Stat1 (Tyr701) Antibody H, M, R, (B, Dg) 5148 H 4486 H
9177 Phospho-Stat1 (Ser727) Antibody H, M, R, (B) 5148 H 4486 H
9172 Stat1 Antibody H, M, R, Mk, (B, Dg) 5148 H 4486 H
9145 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb H, M, R, Mk, (Hm, B, Pg, Hr) 4663 H 4486 H
9131 Phospho-Stat3 (Tyr705) Antibody H, M, R, Mk, (C, B, Dg) 4663 H 4486 H
9134 Phospho-Stat3 (Ser727) Antibody H, M, R, (B) 4663 H 4486 H
4904 Stat3 (79D7) Rabbit mAb H, M, R, Mk 4663 H 4486 H
9139 Stat3 (124H6) Mouse mAb H, M, R, Mk 4663 H 4486 H
9132 Stat3 Antibody H, M, R, Mk, (B) 4663 H 4486 H
5267 Phospho-Stat4 (Tyr693) Antibody H, (M, R) 4486 H
2653 Stat4 (C46B10) Rabbit mAb H, M, R 4486 H
9358 Stat5 (3H7) Rabbit mAb H, M, R 5131 M 7015 M
9351 Phospho-Stat5 (Tyr694) Antibody H, M, (R, B) 5131 M 7015 M
9363 Stat5 Antibody H, M, R, Mk 5131 M 7015 M
3737 SUZ12 (D39F6) XP® Rabbit mAb H, M, R, Mk 7707 H, 5517 H 4486 H
2569 TCF4 (C48H11) Rabbit mAb H, (M, C) 5111 H 4486 H
2565 TCF4 (C9B9) Rabbit mAb H, (M, R) 5111 H 4486 H

Reactivity Key: H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Mi=Mink  Dm=D.melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Sc=S.cerevisiae  Hr=Horse  Ce=C.elegans  All=All Species Expected 
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications