ChIP Kits and Antibodies
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. Cell Signaling Technology® (CST) now has extended its expertise in antibody validation and is providing you with antibodies, kits and reagents for chromatin immunoprecipitation (ChIP).
CST’s new ChIP recommended antibodies have been validated in house by our antibody development scientists by the same rigorous standards as all other recommended applications. Technical support will be provided by the same scientists that validated the antibody, which will guarantee a fast, thorough and accurate response.
CST’s new SimpleChIP™ Enzymatic Chromatin IP Kits are co-developed by CST and NEB scientists and therefore contain the highest quality research reagents. These kits are available with either Protein G agarose or Protein G magnetic beads and contain all buffers and reagents needed to perform up to 30 ChIP assays. The same reagents in these kits have been used for in-house ChIP validation at CST, which will simplify your optimization. The kits can be utilized with any ChIP-validated antibody to detect endogenous levels of protein-DNA interactions and histone modifications in mammalian cells. Protein G agarose and Protein G magnetic beads are also available as separate products.
ChIP Tech Tip
To ensure for the most efficient chromatin immunoprecipitation, it is critical to use both high quality chromatin and ChIP-validated antibodies. It is important to have the correct fragment size and concentration of chromatin. We recommend adding 10–20 μg of chromatin, ranging from 150–700 bp in length, to each immunoprecipitation. We recommend using a ChIP-validated antibody, typically using 1–5 μg of antibody per immunoprecipitation.
ChIP Kits
- SimpleChIP™ Enzymatic Chromatin IP Kit (Agarose Beads) #9002
- SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003
ChIP Companion Products
ChIP Antibodies
| No. | Name | Applications | Reactivity |
|---|---|---|---|
| No. | Name | Applications | Reactivity |
| 9814 | Acetylated-Lysine (Ac-K2-100) Rabbit mAb | W, IP, ChIP, E-P | All |
| 9562 | β-Catenin Antibody | W, IP, IHC-P, ChIP | H, M, R, Mk, Z |
| 9581 | β-Catenin Antibody (Amino-terminal Antigen) | W, IP, IF-F, F, ChIP | H, M, R, Mk, Hm, B |
| 9587 | β-Catenin Antibody (Carboxy-terminal Antigen) | W, IP, IHC-P, IHC-F, IF-F, F, ChIP | H, M, Mk, Mi, Hm, B, (Pg, C, X, Dg) |
| 4771 | Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody | W, IP, ChIP | H, M, R, Mk |
| 9198 | Phospho-CREB (Ser133) (87G3) Rabbit mAb | W, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP | H, M, R |
| 4276 | Phospho-CREB (Ser133) (D1G6) XP™ Rabbit mAb | W, IP, ChIP | H, M, R |
| 9197 | CREB (48H2) Rabbit mAb | W, IP, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP | H, M, R, Mk, Dm |
| 4820 | CREB (D76D11) Rabbit mAb | W, IP, IF-IC, F, ChIP | H, M, R, Mk, Hm, Dm |
| 3417 | CTCF (D1A7) XP™ Rabbit mAb | W, IP, IF-IC, ChIP | H, R, Mk, (B) |
| 3418 | CTCF (D31H2) XP™ Rabbit mAb | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, (B, Hr) |
| 2899 | CTCF Antibody | W, IP, IF-IC, ChIP | H, M, R, Mk |
| 9725 | Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk |
| 9726 | Di-Methyl-Histone H3 (Lys4) Antibody | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, (X, Z) |
| 9751 | Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, Sc, Dm, (X, Z) |
| 4353 | Acetyl-Histone H3 (Lys27) Antibody | W, IP, ChIP | H, M, R, Mk, (C, Hm, B, Dm, X, Z) |
| 9733 | Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, (X, Z) |
| 9756 | Tri-Methyl-Histone H3 (Lys27) Antibody | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, (X) |
| 9649 | Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb | W, IP, IHC-P, IF-IC, F, ChIP | H, M, Z, (R, Mk) |
| 9671 | Acetyl-Histone H3 (Lys9) Antibody | W, IP, IHC-P, ChIP | H, M, R, Mk, Sc, Dm |
| 9677 | Acetyl-Histone H3 (Lys9/Lys14) Antibody | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, Z |
| 9675 | Acetyl-Histone H3 (Lys18) Antibody | W, IHC-P, ChIP | H, M, R |
| 4658 | Di-Methyl-Histone H3 (Lys9) (D85B4) XP™ Rabbit mAb | W, IP, IF-IC, ChIP | H, M, R, Mk, (Pg, Sc, B, Dm, X, Z, Ce) |
| 9753 | Di-Methyl-Histone H3 (Lys9) Antibody | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk, Sc, Dm |
| 4473 | Pan-Methyl-Histone H3 (Lys9) (D54) XP™ Rabbit mAb | W, IP, IF-IC, ChIP | H, M, R, Mk, (Pg, Sc, C, B, Dm, X, Z, Ce) |
| 4069 | Pan-Methyl-Histone H3 (Lys9) Antibody | W, IP, IF-IC, ChIP | H, M, R, Mk, Z |
| 9728 | Di-Methyl-Histone H3 (Lys27) (D18C8) XP™ Rabbit mAb | W, IF-IC, ChIP | H, M, R, Mk |
| 9754 | Tri-Methyl-Histone H3 (Lys9) Antibody | W, IF-IC, ChIP | H, M, R, Mk, (Pg, Dm, Hr) |
| 4909 | Tri-Methyl-Histone H3 (Lys36) (D5A7) XP™ Rabbit mAb | W, ChIP | H, M, R, Mk, (C, Hm, B, Dm, X, Z) |
| 4620 | Histone H3 (D2B12) XP™ Rabbit mAb (ChIP Formulated) | ChIP | H, M, (R, Mk, C, B, Dm, X, Z) |
| 2650 | Histone H3 Antibody (ChIP Formulated) | ChIP | H, M, (R, Mk, C, B, Dm, X, Z) |
| 2594 | Acetyl-Histone H4 (Lys8) Antibody | W, IHC-P, IF-IC, F, ChIP | H, M, R, Mk |
| 2960 | Histone H4 (L64C1) Mouse mAb (ChIP Formulated) | ChIP | H, (M, R, Mk, B, Dm, X, Z, Hr) |
| 4299 | IRF-4 (D43H10) XP™ Rabbit mAb | W, IP, ChIP | H, R |
| 4964 | IRF-4 Antibody | W, IP, IF-IC, F, ChIP | H |
| 9441 | Acetylated-Lysine Antibody | W, IP, IHC-P, IF-IC, ChIP, E-P | All |
| 3619 | MCM2 (D7G11) XP™ Rabbit mAb | W, IP, IHC-P, IF-IC, ChIP | H, M, R, Mk |
| 5605 | c-Myc (D84C12) XP™ Rabbit mAb | W, IP, IF-IC, F, ChIP | H, M, R, (Mk, Pg, Dg) |
| 9402 | c-Myc Antibody | W, IP, ChIP | H, M, R, Pg |
| 2750 | Oct-4 Antibody | W, IHC-P, IF-IC, F, ChIP | H |
| 5677 | Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) | ChIP | H, M |
| 2890 | Oct-4A (C52G3) Rabbit mAb | W, IHC-P, IF-IC, F, ChIP | H |
| 3378 | PCAF (C14G9) Rabbit mAb | W, IP, ChIP | H, M, R, Mk, (B, Hr) |
| 2258 | PU.1 (9G7) Rabbit mAb | W, IP, IHC-P, IF-IC, F, ChIP | H, M, (Mk, Pg) |
| 2266 | PU.1 Antibody | W, IP, IHC-P, IF-IC, F, ChIP | H, M, (Mk, Pg) |
| 2729 | Normal Rabbit IgG | ChIP | |
| 2629 | Rpb1 CTD (4H8) Mouse mAb | W, IP, ChIP | H, M, R, Mk, (Sc, Hm, Dm) |
| 9511 | Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody | W, F, ChIP | H, M, R, Mi, X |
| 9743 | Smad1 Antibody | W, IP, ChIP | H, M, R, Mk |
| 9520 | Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb | W, IP, ChIP | H, (M, R, Mk, B, X, Z) |
| 9523 | Smad3 (C67H9) Rabbit mAb | W, IP, IF-IC, F, ChIP | H, M, R, (Mk, B, X, Z) |
| 9515 | Smad4 Antibody | W, ChIP | H, M, R, Mk |
| 5024 | Sox2 (D6D9) XP™ Rabbit mAb (ChIP Formulated) | ChIP | H, (Mk, B, Dg, Hr) |
| 2748 | Sox2 Antibody | W, IP, ChIP | H, M, (R, Mk, B, Dg, Hr) |
| 9167 | Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb | W, IP, IHC-P, IHC-F, IF-IC, F, ChIP | H, M |
| 9171 | Phospho-Stat1 (Tyr701) Antibody | W, IP, IF-IC, F, ChIP | H, M, R, (B) |
| 9177 | Phospho-Stat1 (Ser727) Antibody | W, IF-IC, F, ChIP | H, M, R |
| 9172 | Stat1 Antibody | W, IP, ChIP | H, M, R, Mk, (B) |
| 9145 | Phospho-Stat3 (Tyr705) (D3A7) XP™ Rabbit mAb | W, IP, IHC-P, IHC-F, IF-IC, F, ChIP | H, M, R, Mk |
| 9131 | Phospho-Stat3 (Tyr705) Antibody | W, IP, IF-IC, ChIP | H, M, R, C, (B) |
| 9134 | Phospho-Stat3 (Ser727) Antibody | W, IP, IF-IC, ChIP | H, M, R, (B) |
| 4904 | Stat3 (79D7) Rabbit mAb | W, IP, IHC-P, ChIP | H, M, R, Mk |
| 9132 | Stat3 Antibody | W, IP, IHC-P, ChIP | H, M, R, (B) |
| 9139 | Stat3 (124H6) Mouse mAb | W, IP, IHC-P, IF-IC, F, ChIP | H, M, R, Mk |
| 9351 | Phospho-Stat5 (Tyr694) Antibody | W, F, ChIP | H, M, (R, B) |
| 9358 | Stat5 (3H7) Rabbit mAb | W, IP, ChIP | H, M, R |
| 9363 | Stat5 Antibody | W, IP, ChIP | H, M, R |
| 3737 | SUZ12 (D39F6) XP™ Rabbit mAb | W, IP, IF-IC, ChIP | H, M, R, Mk |
| 2569 | TCF4 (C48H11) Rabbit mAb | W, IP, ChIP | H, (M, R, C) |
| 2565 | TCF4 (C9B9) Rabbit mAb | W, IP, ChIP | H, (M, R) |
