Cell Signaling Technology

ChIP

ChIP helix

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. Cell Signaling Technology® (CST) now has extended its expertise in antibody validation and is providing you with antibodies, kits and reagents for chromatin immunoprecipitation (ChIP).

CST’s new ChIP recommended antibodies have been validated in house by our antibody development scientists by the same rigorous standards as all other recommended applications. Technical support will be provided by the same scientists that validated the antibody, which will guarantee a fast, thorough and accurate response.

ChIP Magnetic Rack

CST’s new SimpleChIP™ Enzymatic Chromatin IP Kits are co-developed by CST and NEB scientists and therefore contain the highest quality research reagents. These kits are available with either Protein G agarose or Protein G magnetic beads and contain all buffers and reagents needed to perform up to 30 ChIP assays. The same reagents in these kits have been used for in-house ChIP validation at CST, which will simplify your optimization. The kits can be utilized with any ChIP-validated antibody to detect endogenous levels of protein-DNA interactions and histone modifications in mammalian cells. Protein G agarose and Protein G magnetic beads are also available as separate products.

ChIP Tech Tip

To ensure for the most efficient chromatin immunoprecipitation, it is critical to use both high quality chromatin and ChIP-validated antibodies. It is important to have the correct fragment size and concentration of chromatin. We recommend adding 10–20 μg of chromatin, ranging from 150–700 bp in length, to each immunoprecipitation. We recommend using a ChIP-validated antibody, typically using 1–5 μg of antibody per immunoprecipitation.

ChIP Kits

Companion Products

ChIP Product Image

ChIP Validated Antibodies

No. Name Applications Reactivity
No. Name Applications Reactivity
9562 β-Catenin Antibody W, IP, IHC-P, ChIP H, M, R, Mk, Z
9581 β-Catenin Antibody (Amino-terminal Antigen) W, IP, IF-F, F, ChIP H, M, R, Mk, Hm, B
9587 β-Catenin Antibody (Carboxy-terminal Antigen) W, IP, IHC-P, IHC-F, IF-F, F, ChIP H, M, Mk, Mi, Hm, B, (Pg, C, X, Dg)
4771 Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody W, IP, ChIP H, M, R, Mk
9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb W, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP H, M, R
9197 CREB (48H2) Rabbit mAb W, IP, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP H, M, R, Mk, Dm
3417 CTCF (D1A7) Rabbit mAb W, IP, IF-IC, ChIP H, R, Mk, (B)
3418 CTCF (D31H2) Rabbit mAb W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, (B)
2899 CTCF Antibody W, IP, IF-IC, ChIP H, M, R, Mk
9725 Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk
9726 Di-Methyl-Histone H3 (Lys4) Antibody W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, (X, Z)
9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, Sc, Dm, (X, Z)
9733 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, (X, Z)
9756 Tri-Methyl-Histone H3 (Lys27) Antibody W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, (X)
9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb W, IP, IHC-P, IF-IC, F, ChIP H, M, Z, (R, Mk)
9671 Acetyl-Histone H3 (Lys9) Antibody W, IP, IHC-P, ChIP H, M, R, Mk, Sc, Dm
9677 Acetyl-Histone H3 (Lys9/Lys14) Antibody W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, Z
9675 Acetyl-Histone H3 (Lys18) Antibody W, IHC-P, ChIP H, M, R
9753 Di-Methyl-Histone H3 (Lys9) Antibody W, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, Sc, Dm
4069 Pan-Methyl-Histone H3 (Lys9) Antibody W, IP, IF-IC, ChIP H, M, R, Mk, Z
9728 Di-Methyl-Histone H3 (Lys27) (D18C8) Rabbit mAb W, IF-IC, ChIP H, M, R, Mk
9754 Tri-Methyl-Histone H3 (Lys9) Antibody W, IF-IC, ChIP H, M, R, Mk, (Pg, Dm)
2650 Histone H3 Antibody (ChIP Formulated) ChIP H, M, (R, Mk, C, B, Dm, X, Z)
2594 Acetyl-Histone H4 (Lys8) Antibody W, IHC-P, IF-IC, F, ChIP H, M, R, Mk
2960 Histone H4 (L64C1) Mouse mAb (ChIP Formulated) ChIP H, (M, R, Mk, B, Dm, X, Z)
4964 IRF-4 Antibody W, IP, IF-IC, F, ChIP H
9441 Acetylated-Lysine Antibody W, IP, IHC-P, IF-IC, ChIP, E-P All
3378 PCAF (C14G9) Rabbit mAb W, IP, ChIP H, M, R, Mk, (B)
2258 PU.1 (9G7) Rabbit mAb W, IP, IHC-P, IF-IC, F, ChIP H, M, (Mk, Pg)
2266 PU.1 Antibody W, IP, IHC-P, IF-IC, F, ChIP H, M, (Mk, Pg)
2729 Normal Rabbit IgG ChIP
2629 Rpb1 CTD (4H8) Mouse mAb W, IP, ChIP H, M, R, Mk, (Sc, Hm, Dm)
9520 Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb W, IP, ChIP H, (M, R, Mk, B, X, Z)
9523 Smad3 (C67H9) Rabbit mAb W, IP, IF-IC, F, ChIP H, M, R, (Mk, B, X, Z)
9515 Smad4 Antibody W, ChIP H, M, R, Mk
9167 Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F, ChIP H, M
9171 Phospho-Stat1 (Tyr701) Antibody W, IP, IF-IC, F, ChIP H, M, R, (B)
9177 Phospho-Stat1 (Ser727) Antibody W, IF-IC, F, ChIP H, M, R
9172 Stat1 Antibody W, IP, ChIP H, M, R, Mk, (B)
9145 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F, ChIP H, M, R, Mk
9131 Phospho-Stat3 (Tyr705) Antibody W, IP, IF-IC, ChIP H, M, R, C, (B)
9134 Phospho-Stat3 (Ser727) Antibody W, IP, IF-IC, ChIP H, M, R, (B)
4904 Stat3 (79D7) Rabbit mAb W, IP, IHC-P, ChIP H, M, R, Mk
9132 Stat3 Antibody W, IP, IHC-P, ChIP H, M, R, (B)
9139 Stat3 (124H6) Mouse mAb W, IP, IHC-P, IF-IC, F, ChIP H, M, R, Mk
9351 Phospho-Stat5 (Tyr694) Antibody W, F, ChIP H, M, (R, B)
9358 Stat5 (3H7) Rabbit mAb W, IP, ChIP H, M, R
9363 Stat5 Antibody W, IP, ChIP H, M, R
3737 SUZ12 (D39F6) Rabbit mAb W, IP, IF-IC, ChIP H, M, R, Mk
2569 TCF4 (C48H11) Rabbit mAb W, IP, ChIP H, (M, R, C)
2565 TCF4 (C9B9) Rabbit mAb W, IP, ChIP H, (M, R)

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