SimpleChIP^® Control PCR Primers

SimpleChIP^® Control PCR Primers are a mix of two control primers which can be used to amplify DNA that has been isolated using ChIP. These primers will amplify positive control DNA sequences that contain known binding sites of the target protein detected by the antibody employed in the ChIP assay, and can also be used as negative control to demonstrate antibody sensitivity.

Primers are designed, tested, and optimized in-house in conjunction with our ChIP validated antibodies and SimpleChIP^® Kits, saving time and reagents.
Primers are optimized for use in real-time PCR with SYBR^® Green dye, which simplifies quantification of DNA enrichment.
Technical Support is provided by the scientists who designed and use these products, and know them best.

SimpleChIP^® Human GAPDH Promoter Primers #4471

DNA isolated from cross-linked cells using the SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) #9003.

Figure 1. Amplification - SimpleChIP^® Human GAPDH Promoter Primers #4471 were tested on DNA isolated from cross-linked cells using the SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR^® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R²) were calculated based on the corresponding threshold cycle (C_T) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

PCR product melting curves using SimpleChIP Human GAPDH Promoter Primers #4471.

Figure 2. Specificity - PCR product melting curves were obtained on real-time PCR reactions performed using SimpleChIP^® Human GAPDH Promoter Primers #4471. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.

SimpleChIP^® Control PCR Primers

No.	Name	Applications	Reactivity
No.	Name	Applications	Reactivity
5037	SimpleChIP^® Human γ-Actin Promoter Primers	ChIP	H
5047	SimpleChIP^® Human γ-Actin Intron 3 Primers	ChIP	H
5098	SimpleChIP^® Human AFM Intron 1 Primers	ChIP	H
7269	SimpleChIP^® Mouse AFM Intron 2 Primers	ChIP	M
4486	SimpleChIP^® Human α Satellite Repeat Primers	ChIP	H
12376	SimpleChIP^® Human RUNX2 Promoter Primers	ChIP	H
8973	SimpleChIP^® Human Axin2 Intron 1 Primers	ChIP	H
5111	SimpleChIP^® Human CaMK2D Intron 3 Primers	ChIP	H
7983	SimpleChIP^® Rat CCRN4L Promoter Primers	ChIP	R
5131	SimpleChIP^® Mouse CIS Intron 1 Primers	ChIP	M
7531	SimpleChIP^® Human DHFR Intron 1 Primers	ChIP	H
7710	SimpleChIP^® Human DMD Intron 2 Primers	ChIP	H
5549	SimpleChIP^® Human EGR1 Promoter Primers	ChIP	H
11953	SimpleChIP^® Human EGR1 Intron 3 Primers	ChIP	H
9673	SimpleChIP^® Human ESR1 Promoter Primers	ChIP	H
7859	SimpleChIP^® Human FKBP51 Intron 5 Primers	ChIP	H
4663	SimpleChIP^® Human c-Fos Promoter Primers	ChIP	H
12010	SimpleChIP^® Human c-Fos Exon 3 Primers	ChIP	H
4471	SimpleChIP^® Human GAPDH Promoter Primers	ChIP	H
4478	SimpleChIP^® Human GAPDH Intron 2 Primers	ChIP	H
5516	SimpleChIP^® Human GAPDH Exon 1 Primers	ChIP	H
8986	SimpleChIP^® Mouse GAPDH Intron 2 Primers	ChIP	M
7964	SimpleChIP^® Rat GAPDH Promoter Primers	ChIP	R
5550	SimpleChIP^® Human GATA-6 Promoter Primers	ChIP	H
7273	SimpleChIP^® Human HES4 Promoter Primers	ChIP	H
7707	SimpleChIP^® Human HoxA1 Intron 1 Primers	ChIP	H
7341	SimpleChIP^® Mouse HoxA1 Promoter Primers	ChIP	M
5517	SimpleChIP^® Human HoxA2 Promoter Primers	ChIP	H
7429	SimpleChIP^® Mouse HoxD10 Exon 1 Primers	ChIP	M
5551	SimpleChIP^® Human HSPA6 Promoter Primers	ChIP	H
5139	SimpleChIP^® Human ID1 Promoter Primers	ChIP	H
5172	SimpleChIP^® Human H19/Igf2 Imprinting Control Region Primers	ChIP	H
5552	SimpleChIP^® Human IκBα Promoter Primers	ChIP	H
12377	SimpleChIP^® Human MITF Promoter Primers	ChIP	H
9013	SimpleChIP^® Human MS4A7 Promoter Primers	ChIP	H
4490	SimpleChIP^® Human MyoD1 Exon 1 Primers	ChIP	H
4493	SimpleChIP^® Human MYT-1 Exon 1 Primers	ChIP	H
8985	SimpleChIP^® Mouse MYT-1 Promoter Primers	ChIP	M
4779	SimpleChIP^® Human NPM1 Intron 1 Primers	ChIP	H
12635	SimpleChIP^® Mouse NQO1 Promoter Primers	ChIP	M
4829	SimpleChIP^® Human NR4A3 Promoter Primers	ChIP	H
4641	SimpleChIP^® Human Oct-4 Promoter Primers	ChIP	H
4653	SimpleChIP^® Mouse Oct-4 Promoter Primers	ChIP	M
4669	SimpleChIP^® Human CDKN1A Intron 1 Primers	ChIP	H
6449	SimpleChIP^® Human CDKN1A Promoter Primers	ChIP	H
11951	SimpleChIP^® Human CDKN1B Promoter Primers	ChIP	H
12281	SimpleChIP^® Human EP300 Promoter Primers	ChIP	H
9014	SimpleChIP^® Human PRF1 Promoter Primers	ChIP	H
8984	SimpleChIP^® Mouse PITX3 Intron 1 Primers	ChIP	M
7014	SimpleChIP^® Human RPL30 Exon 3 Primers	ChIP	H
7015	SimpleChIP^® Mouse RPL30 Intron 2 Primers	ChIP	M
5077	SimpleChIP^® Human Sat2 Repeat Element Primers	ChIP	H
7681	SimpleChIP^® Human SLC19A2 Promoter Primers	ChIP	H
4649	SimpleChIP^® Human Sox2 Promoter Primers	ChIP	H
5156	SimpleChIP^® Human SUB1 Promoter Primers	ChIP	H
5148	SimpleChIP^® Human TAP1 Promoter Primers	ChIP	H
9702	SimpleChIP^® Human pS2 Promoter Primers	ChIP	H
7001	SimpleChIP^® Human Timeless Intron 1 Primers	ChIP	H
4659	SimpleChIP^® Mouse XIST Intron 1 Primers	ChIP	M
12558	SimpleChIP^® Human PMAIP1 Intron 1 Primers	ChIP	H

Applications Key: ChIP=Chromatin Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat