SignalStain® Boost IHC Detection Reagents
SignalStain® Boost IHC Detection Reagents
Cell Signaling Technology® is proud to introduce SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 and (HRP, Mouse) #8125. These polymer-based detection reagents were developed to enhance sensitivity and eliminate complications arising from false positive staining due to endogenous biotin. Both products offer a one-step, highly sensitive alternative to traditional IHC detection methods. SignalStain® Boost IHC Detection Reagents are specific to either rabbit or mouse IgG, can be used to visualize targets in both paraffin-embedded and frozen tissue, and are compatible with all peroxidase-based substrates.
Cell Signaling Technology’s new polymer-based SignalStain® Boost IHC Detection Reagent offers multiple advantages over conventional ABC detection methods:
- Superior sensitivity, resulting in a stronger signal.
- A reduced number of steps in the detection procedure, saving valuable time.
- Less false positives resulting from endogenous biotin staining, providing a lower overall background.
Nanog (1E6C4) Mouse mAb #4893: IHC analysis of paraffin-embedded human seminoma using #4893 with biotin based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125 (right).
Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077: IHC analysis of paraffin-embedded human papillary renal cell carcinoma using #3077 with biotin based detection (left) or SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 (right).
SignalStain® Boost IHC Detection Reagents
| No. | Name |
|---|---|
| 8125 | SignalStain® Boost IHC Detection Reagent (HRP, Mouse) |
| 8114 | SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) |
**Some antibodies may require titration when used with this highly sensitive detection reagent. Please refer to the individual product datasheet to determine whether your antibody of interest has been optimized with SignalStain® Boost IHC Detection Reagent.
