Motif Antibodies
Introduction
Motif specific antibodies are proprietary to CST (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents) and are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific kinase substrate, binding motif, or other phosphorylation motifs. These phospho-motif antibodies are useful to identify and quantify substrates and biomarkers for kinases from many branches of the human kinome.
Motif antibodies can be employed in a variety of platforms to detect specific phosphorylated protein sequences and can be used in combination with separation analysis such as 2D gel SDS PAGE to characterize or identify specific phosphoproteins.
These antibodies can also be applied in conjunction with PhosphoScan®, CST’s proprietary masspectrometry technology for post-translational modification site discovery and quantification.
Motif antibodies can also be used for drug discovery as detection antibodies for in vitro enzymatic assays including kinase and phosphatase assays.
Product Categories
- Phospho-Serine
- Phospho-Threonine
- Phospho-Serine/Threonine and Related Antibodies
- Phospho-Tyrosine
- Other Motifs
Phospho-Serine
| Product | Antibody Name | Applications | Motif Recognized |
|---|---|---|---|
| 9615 | Phospho-(Ser) Kinase Substrate Antibody Sampler Kit‡ | ||
| 9601 | Phospho-(Ser) 14-3-3 Binding Motif Antibody‡ | W, IP, IHC-P, E-P | (R/K)XX(S*)XP |
| 9606 | Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb‡ | W, IP, E-P | (R/K)XX(S*)XP |
| 2324 | Phospho-(Ser) CDKs Substrate Antibody‡ | W, IP, IHC-P, E-P | (K/R)(S*)PX(K/R) |
| 2325 | Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb‡ | W, IP, IHC-P, E-P | PXsPXR/K-S-P |
| 2261 | Phospho-(Ser) PKC Substrate Antibody‡ | W, IP, E-P | (R/K)X(S*)(Hyd)(R/K) |
| 2981 | Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody‡ | W, IP, E-P | RX(Y/F)X(S*) |
Phospho-Threonine
| Product | Antibody Name | Applications | Motif Recognized |
|---|---|---|---|
| 2321 | Phospho-(Thr) MAPK/CDK Substrate Mouse mAb‡ | W, IHC-P, E-P | (T*)P |
| 9391 | Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)‡ | W, IHC-P, E-P | (T*)P |
| 4391 | Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb‡ | W, IP, IHC-P, F, E-P | PXS(T)PXR-P |
| 9381 | Phospho-Threonine Antibody (P-Thr-Polyclonal)‡ | W, IP, IHC-P, E-P | (T*) |
| 2351 | Phospho-Threonine-X-Arginine Antibody‡ | W, IHC-P, E-P | (T*)X(R/K) |
| 9386 | Phospho-Threonine (42H4) Mouse mAb‡ | W, IP, E-P | (T*) |
Phospho-Serine/Threonine and Related Antibodies
| Product | Antibody Name | Applications | Motif Recognized |
|---|---|---|---|
| 9920 | Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit‡ | ||
| 9619 | Immobilized Phospho-(Ser/Thr) Akt Substrate Antibody‡ | IP | (R/K)X(R/K)XX(T*/S*) |
| 9611 | Phospho-(Ser/Thr) Akt Substrate Antibody‡ | W, IP, IHC-P, E-P | (R/K)X(R/K)XX(T*/S*) |
| 9614 | Phospho-Akt Substrate (RXRXXS/T) (110B7E) Rabbit mAb‡ | W, IP, IHC-P, E-P | (R/K)X(R/K)XX(T*/S*) |
| 2909 | Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb‡ | W | L(S*)Q |
| 2851 | Phospho-(Ser/Thr) ATM/ATR Substrate Antibody‡ | W, IP, IHC-P, E-P | L(S*)Q |
| 2321 | Phospho-(Thr) MAPK/CDK Substrate Mouse mAb‡ | W, IHC-P, E-P | (T*)P |
| 9391 | Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)‡ | W, IHC-P, E-P | (T*)P |
| 4391 | Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb‡ | W, IP, IHC-P, F, E-P | PXS(T)PXR-P |
| 9634 | Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb‡ | W, IP, E-P | (F/K)XX(F/Y)(S*/T*)(F/Y) |
| 9631 | Phospho-(Ser/Thr) Phe Antibody‡ | W, IP, E-P | (F/Y/W)(S*/T*)(F) |
| 9621 | Phospho-(Ser/Thr) PKA Substrate Antibody‡ | W, IP, IHC-P, E-P | RRXS*/T* |
| 9624 | Phospho-PKA Substrate (RRXS/T) (100G7E) Rabbit mAb‡ | W, IP, E-P | RRXS*/T* |
| 2351 | Phospho-Threonine-X-Arginine Antibody‡ | W, IHC-P, E-P | (T*)X(R/K) |
Phospho-Tyrosine
| Product | Antibody Name | Applications | Motif Recognized |
|---|---|---|---|
| 3821 | Phospho-(Tyr) p85 PI3K Binding Motif Antibody‡ | W, IP, IHC-P, E-P | (Y*)XXM |
| 9419 | Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100)‡ | IP | Y* |
| 9414 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)‡ | F | Y* |
| 9415 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)‡ | IF-IC, F | Y* |
| 9417 | Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)‡ | W, IP, IHC-P | Y* |
| 9411 | Phospho-Tyrosine Mouse mAb (P-Tyr-100)‡ | W, IP, IHC-P, IF-F, IF-IC, IF-P, F, E-P | Y* |
| 9418 | Phospho-Tyrosine Mouse mAb (P-Tyr-102) (Fluorescein Conjugate)‡ | IF-IC, F | Y* |
| 9416 | Phospho-Tyrosine Mouse mAb (P-Tyr-102)‡ | W, IP, IHC-P, F, E-P | Y* |
Other Motifs
| Product | Antibody Name | Applications | Motif Recognized |
|---|---|---|---|
| 9441 | Acetylated-Lysine Antibody‡ | W, IP, IHC-P, IF-IC, ChIP, E-P | Ac-K |
| 9681 | Acetylated-Lysine Mouse mAb (Ac-K-103)‡ | W, E-P | Ac-K |
| 9691 | Nitro-Tyrosine Antibody‡ | W | CXXXXXX(Yno2)XXXXXXX |
‡Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Business Development at cbunker@cellsignal.com.
