Motif Antibodies

Introduction

Motif specific antibodies are proprietary to CST (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents) and are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific kinase substrate, binding motif, or other phosphorylation motifs. These phospho-motif antibodies are useful to identify and quantify substrates and biomarkers for kinases from many branches of the human kinome.

Motif antibodies can be employed in a variety of platforms to detect specific phosphorylated protein sequences and can be used in combination with separation analysis such as 2D gel SDS PAGE to characterize or identify specific phosphoproteins.

These antibodies can also be applied in conjunction with PhosphoScan^®, CST’s proprietary masspectrometry technology for post-translational modification site discovery and quantification.

Motif antibodies can also be used for drug discovery as detection antibodies for in vitro enzymatic assays including kinase and phosphatase assays.

Product Categories

• Phospho-Serine
• Phospho-Threonine
• Phospho-Serine / Threonine
• Phospho-Tyrosine
• Nitro-Tyrosine
• Acetylated-Lysine
• Mono-Methyl-Arginine

PhosphoScan Motif Antibodies

View the applications key.

Phospho-Serine

No.	Antibody Name	Applications	Motif Recognized
9615	Phospho-(Ser) Kinase Substrate Antibody Sampler Kit‡
9606	Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb‡	W, IP, E-P	(K/R)XXS*P
9601	Phospho-(Ser) 14-3-3 Binding Motif Antibody‡	W, IP, IHC-P, E-P	(K/R)XXS*P
9607	Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb‡	W, IP	S*Q
6967	Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb‡	W, IP, E-P	(K/R)XS*X(K/R)
2325	Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb‡	W, IP, IHC-P, E-P	PXS*P, S*PX(K/R)
5501	Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate)‡	IP	PXS*P, S*PX(R/K)
9477	Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb‡	W, IP, E-P	(K/H)S*P
2261	Phospho-(Ser) PKC Substrate Antibody‡	W, IP, E-P	(K/R)XS*(Hyd)(K/R)
2981	Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody‡	W, IP, E-P	RX(F/Y)XS*

Phospho-Threonine

No.	Antibody Name	Applications	Motif Recognized
2321	Phospho-(Thr) MAPK/CDK Substrate Mouse mAb‡	W, IHC-P, E-P	T*P
9391	Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)‡	W, IHC-P, E-P	T*P
5243	Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb‡	W, IP, E-P	ST*P
2351	Phospho-Threonine-X-Arginine Antibody‡	W, IHC-P, E-P	T*X(K/R)
3004	Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb‡	W, IP, E-P	T*PE, T*P
9386	Phospho-Threonine (42H4) Mouse mAb‡	W, IP, E-P	T*
9381	Phospho-Threonine Antibody (P-Thr-Polyclonal)‡	W, IP, E-P	T*
8781	Phospho-Threonine Antibody (P-Thr-Polyclonal) (Sepharose Bead Conjugate)‡	IP	T*
6949	Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate)‡	W, E-P	T*

Phospho-Serine / Threonine

No.	Antibody Name	Applications	Motif Recognized
9920	Phospho-(Ser/Thr) Kinase Substrate Antibody Sampler Kit‡
9614	Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb‡	W, IP, IHC-P, E-P	RXX(S*/T*)
6950	Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate)‡	W	RXX(S*/T*)
8050	Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Magnetic Bead Conjugate)‡	IP	RXX(S*/T*)
9646	Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate)‡	IP	RXX(S*/T*)
9611	Phospho-(Ser/Thr) Akt Substrate Antibody‡	W, IP, IHC-P, E-P	(K/R)XX(S*/T*)
10001	Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb‡	W, E-P	RXRXX(S*/T*)
5759	Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb‡	W, IP, E-P	(L/M)XRXX(S*/T*), RXX(S*/T*)
2909	Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb‡	W	L(S*/T*)Q
2851	Phospho-(Ser/Thr) ATM/ATR Substrate Antibody‡	W, IP, IHC-P, E-P	L(S*/T*)Q
6966	Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb‡	W, IP	(S*/T*)QG, (S*/T*)Q
8738	Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb‡	W	(S*/T*)DXE
9634	Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb‡	W, IP, E-P	(F/K)XX(F/Y)(S*/T*)(F/Y)
9631	Phospho-(Ser/Thr) Phe Antibody‡	W, IP, E-P	(F/Y/W)(S*/T*)(F)
9624	Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb‡	W, IP, E-P	(K/R)(K/R)X(S*/T*)
9621	Phospho-(Ser/Thr) PKA Substrate Antibody‡	W, IP, IHC-P, E-P	(K/R)(K/R)X(S*/T*)
4381	Phospho-(Ser/Thr) PKD Substrate Antibody‡	W, E-P	LXRXX(S*/T*)

Phospho-Tyrosine

No.	Antibody Name	Applications	Motif Recognized
8954	Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb‡	W, IP, IF-IC, F	Y*
9411	Phospho-Tyrosine Mouse mAb (P-Tyr-100)‡	W, IP, IHC-P, IF-P, IF-F, IF-IC, F, E-P	Y*
9414	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 488 Conjugate)‡	F	Y*
9415	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor® 647 Conjugate)‡	IF-IC, F	Y*
9417	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)‡	W, IP, IHC-P	Y*
5465	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate)‡	W, E-P	Y*
8095	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Magnetic Bead Conjugate)‡	IP	Y*
9419	Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)‡	IP	Y*
9416	Phospho-Tyrosine Mouse mAb (P-Tyr-102)‡	W, IP, IHC-P, F, E-P	Y*

Nitro-Tyrosine

No.	Antibody Name	Applications	Motif Recognized
9691	Nitro-Tyrosine Antibody‡	W	Y-NO2

Acetylated-Lysine

No.	Antibody Name	Applications	Motif Recognized
9814	Acetylated-Lysine (Ac-K2-100) Rabbit mAb‡	W, IP, ChIP, E-P	Ac-K
6952	Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate)‡	W	Ac-K
9681	Acetylated-Lysine Mouse mAb (Ac-K-103)‡	W, E-P	Ac-K
9441	Acetylated-Lysine Antibody‡	W, IP, IHC-P, IF-IC, ChIP, E-P	Ac-K

Mono-Methyl-Arginine

No.	Antibody Name	Applications	Motif Recognized
8015	Mono-Methyl Arginine (Me-R4-100) Rabbit mAb‡	W, IP, E-P	Me-R
8711	Mono-Methyl Arginine (R*GG) (D5A12) Rabbit mAb‡	W, IP, E-P	Me-RGG