PhosphoScan® Motif Antibodies
PhosphoScan® Profiling Services Provide:
- Broad proteomic analysis of many branches of the human kinome
- High affinity enrichment of a broad spectrum of phosphorylated sequences corresponding to substrates of diverse kinases
- Focused analysis of characterized kinases and biomarkers of kinase inhibitor activity
- Broad-spectrum analysis of uncharacterized kinases with the full panel of Cell Signaling Technology’s Phospho-Motif Antibodies
- Discovery and validation of novel phosphorylation sites, substrates and potential biomarkers of target kinases
Cellular Phosphorylation, Acetylation, and Ubiquitination Profiling Services
Cell Signaling Technology (CST) developed PhosphoScan as a novel, kinase-centric proteomic technology for identifying and quantifying sites of post-translational modification. PhosphoScan employs an immunoaffinity strategy to enrich post-translationally modified peptides from complex mixtures and has been used with great success to profile tyrosine phosphorylation. PhosphoScan is also uniquely capable of senine/threonin kinase class focused proteomics by using proprietary antibodies directed to kinase recognition motifs. Furthermore, CST’s proteomics technology is the only global methodology for interrogating other post-translational modifications, such as acetylation and ubiquitination.
CST provides PhosphoScan services employing any of the 19 motif antibodies listed below. Phospho-Motif antibodies are designed to identify and quantify substrates and biomarkers for kinases from many branches of the human kinome. In addition, CST can apply similar motif antibodies to profile broad changes in lysine acetylation and ubiquitination. With this collection of motif antibodies, PhosphoScan provides a powerful strategy for in-depth profiling and quantification of cellular substrates and pathways regulated by specific kinase families.
Phospho-Motif Antibodies for PhosphoScan® Profiling
| Target | Consenus Site | Example Study | Study Sample Type | Phosphopeptides Identified |
|---|---|---|---|---|
| Phospho-Akt Substrate | RXXS/T | #3230 | Mouse brain | 237 |
| Phospho-Akt Substrate | RXRXXS/T | #2964 | MKN-45 (gastric cancer) | 174 |
| Phospho-(Ser/Thr) ATM/ATR Substrate | S/TQ | #3235 | Jurkat (T cell acute lymphoblastic leukemia) | 342 |
| Phospho-(Ser/Thr) ATM/ATR Substrate | S/TQG | #2796 | Jurkat (T cell acute lymphoblastic leukemia) | 414 |
| Phospho-(Ser) CDKs Substrate | (K/R)SPX(K/R) | #2724 | K562 (chronic myelogenous leukemia) | 305 |
| Phospho-(Thr) CK Substrate | T(D/E)X(D/E) | #2921 | Mouse liver | 497 |
| Phospho-MAPK/CDK Substrates | PXSP | #1915 | Kyse140 (esophageal carcinoma) | 459 |
| Phospho-MAPK Substrates | PXTP | #1826 | SEM (B cell acute lymphoblastic leukemia) | 260 |
| Phospho-(Ser/Thr) PDK1 Docking Motif | (F/Y)S/T(F/Y) | #3121 | Mouse brain | 424 |
| Phospho-PKA Substrate | (K/R)(K/R)X(S/T) | #2929 | Jurkat (T cell acute lymphoblastic leukemia) | 205 |
| Phospho-(Ser) PKC Substrate | (K/R)XSX(K/R) | #2628 | K562 (chronic myelogenous leukemia) | 152 |
| Phospho-(Ser/Thr) PKD Substrate | LXRXXS/T | #2901 | Mouse brain | 276 |
| Phospho-(Thr) PLK Binding Motif A7907 | S/TP | #1665 | H1703 (non-small cell lung cancer) | 538 |
| Phospho-Thr-Pro Motif | TP, TPP | #3481 | Jurkat (T cell acute lymphoblastic leukemia) | 578 |
| Phospho-Thr-Pro-Glu Motif | TPE, TP | #2544 | Jurkat (T cell acute lymphoblastic leukemia) | 465 |
| Phospho-Thr-X-Arg Motif | TXR, TPR | #2655 | K562 (chronic myelogenous leukemia) | 82 |
| Phosphotyrosine (P-Tyr-100) | Tyrosine | #8809 | H3255 (non-small cell lung cancer) | 930 |
Acetyl- and Ubiquitin-Motif Antibodies for AcetylScan and UbiquiScan Profiling
| Target | Consenus Site | Example Study | Sample Type | Peptides Identified |
|---|---|---|---|---|
| Acetylated-Lysine | K-epsilon-Acetyl | #2292 | Jurkat (T cell acute lymphoblastic leukemia) | Acetylated peptides identified: 283 |
| Ubiquitin Branch | K-epsilon-GG | #3115 | Mouse liver | Ubiquitinated peptides identified: 798 |
