eXceptional Reproducibility of XP® Monoclonal Antibodies
eXceptional Reproducibility of XP® Monoclonal Antibodies
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370, and Jak2 (D2E12) XP® Rabbit mAb #3230 exemplify consistent performance of XP® monoclonal antibodies from lot to lot. Each new lot is always compared to the previous lot in all recommended applications before it becomes available to our customers to ensure the greatest possible consistency in research results.
- Reproducibility 1: Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145
- Reproducibility 2: Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370
- Reproducibility 3: Jak2 (D2E12) XP® Rabbit mAb #3230
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145
Figure 1. Western blot analysis of HeLa cells, untreated or treated with IFN-α comparing lots 1, 2, 3, and 8 of #9145, showing that signal remains consistent from lot to lot. Note: Recommended dilution for western blot was changed to 1:2000 with release of lot 3.
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370
Figure 2. IHC analysis of adjacent sections of paraffin-embedded human colon carcinoma using #4370 lot 7 at a 1:400 dilution (upper left), #4370 lot 9 at a 1:200 dilution (upper right), #4370 lot 9 at a 1:400 dilution (lower left), and #4370 lot 9 at a 1:800 dilution (lower right). The recommended dilution for lot 9 remained 1:400.
Jak2 (D2E12) XP® Rabbit mAb #3230
Figure 3. Western blot analysis of K562 cells comparing lots 1, 3, and 5 of #3230, showing that signal remains consistent from lot to lot.
