Case Study 3: eXceptional Reproducibility of XP® Monoclonal Antibodies
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 and Jak2 (D2E12) XP® Rabbit mAb #3230
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 and Jak2 (D2E12) XP® Rabbit mAb #3230 exemplify the lot-to-lot consistency achieved using XMT® technology coupled with the stringent quality control standards applied to all antibodies from Cell Signaling Technology.
Figure 2. Western blot analysis of K562 cells comparing lot 1, 3 and 5 of Jak2 (D2E12) XP® Rabbit mAb #3230, showing that signal remains consistent from lot to lot.
Figure 1. Western blot analysis of HeLa cells, untreated or treated with IFN-α comparing lot 1, 2, 3 and 8 of Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145, showing that signal remains consistent from lot to lot. Note: Recommended dilution for western blot was changed to 1:2000 with release of lot 3.
