New Site Discovery Initiative at Cell Signaling Technology

Cell Signaling Technology (CST) now offers a growing number of antibodies directed against newly identified protein modification sites (phosphorylation, acetylation, etc.). These sites were first identified by CST scientists using PTMScan^®, CST’s proprietary mass spectrometry platform for post-translational modification site discovery. PTMScan^® employs motif antibodies for peptide immunoaffinity purification from cell extracts combined with LC tandem mass spectrometry to identify and quantify changes in modification-state levels. Motif antibodies employed are specific to phospho- (Tyr, Ser, Thr), acetyl-peptides or ubiquitin-tagged peptides, allowing the analysis of phosphorylation, acetylation or ubiquitinylation status of the cell.

More information on these and other novel modification sites discovered at CST, including many additional publication references, can be found in PhosphoSitePlus^®, CST’s expert-curated modification site knowledgebase (www.phosphosite.org) by clicking on the PhosphoSitePlus ID link.

Applications Key: W=Western blot  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow cytometry 
Reactivity Key: H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Hr=Horse 
Species enclosed in parentheses are predicted to react based on 100% sequence homology.