Immunohistochemical analysis of paraffin-embedded mouse colon using Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated).Learn more about how we get our images.
Immunohistochemical analysis of paraffin-embedded mouse heart using Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated). Heart is negative, as expected.Learn more about how we get our images.
Immunohistochemical analysis of paraffin-embedded mouse spleen using Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
posted February 2010
revised March 2016
Protocol Id: 283
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Ki-67 (D3B5) Rabbit mAb (Mouse Preferred; IHC Formulated) recognizes endogenous levels of murine Ki-67 protein. It will also detect endogenous levels of human Ki-67 protein; however, Ki-67 (D2H10) Rabbit mAb (IHC Specific) #9027 is recommended for the detection of human Ki-67 protein in paraffin-embedded tissues.
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of Ki-67 protein.
Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).
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