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12666
Endosomal Marker Antibody Sampler Kit
Primary Antibodies

Endosomal Marker Antibody Sampler Kit, UniProt ID P20339, Entrez ID 1213 #12666

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Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb.

Western blot analysis of extracts from various cell lines using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb.

Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Rab5 (E6N8S) Mouse mAb.

Western blot analysis of extracts from various cell lines using Rab7 (D95F2) XP® Rabbit mAb.

Western blot analysis of extracts from various cell lines using Rab11 (D4F5) XP® Rabbit mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Immunoprecipitation of caveolin-1 from HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input.

Confocal immunofluorescent analysis of SH-SY5Y cells using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Immunoprecipitation of Rab5 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, lane 3 is Rab5 (E6N8S) Mouse mAb without HeLa cell extracts, and lane 4 is Rab5 (E6N8S) Mouse mAb. Western blot analysis was performed using Rab5 (E6N8S) Mouse mAb.

Confocal immunofluorescent analysis of SK-MEL-28 cells using Rab7 (D95F2) XP® Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of A549 cells using Rab11 (D4F5) XP® Rabbit mAb (green). Actin filaments were labeled wtih DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Caveolin-1 (D46G3) XP® Rabbit mAb.

Immunoprecipitation of Rab5 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is Rab5 (E6N8S) Mouse mAb. Western blot analysis was performed using Rab5 (C8B1) Rabbit mAb #3547.

Immunohistochemical analysis of paraffin-embedded human lymphoma using Caveolin-1 (D46G3) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using Rab5 (E6N8S) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded mouse lung using Caveolin-1 (D46G3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using Rab5 (E6N8S) Mouse mAb.

Flow cytometric analysis of Hela cells using Caveolin-1 (D46G3) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Rab5 (E6N8S) Mouse mAb (left) compared to concentration matched Mouse (G3A1) IgG1 Isotype Control #5415 (right).

Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 (D46G3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Rab5 (E6N8S) Mouse mAb.

Confocal immunofluorescent analysis of HeLa cells using Rab5 (E6N8S) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

To Purchase # 12666T
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Caveolin-1 (D46G3) XP® Rabbit mAb 3267 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Hm Mk B Dg 21, 24 Rabbit IgG
Clathrin Heavy Chain (D3C6) XP® Rabbit mAb 4796 20 µl
  • WB
  • IP
  • IF
H M R Mk 190 Rabbit IgG
EEA1 (C45B10) Rabbit mAb 3288 20 µl
  • WB
  • IP
  • IF
H M R 170 Rabbit IgG
Rab5 (E6N8S) Mouse mAb 46449 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R Mk 25 Mouse IgG1
Rab7 (D95F2) XP® Rabbit mAb 9367 20 µl
  • WB
  • IP
  • IF
H M R Mk 23 Rabbit IgG
Rab11 (D4F5) XP® Rabbit mAb 5589 20 µl
  • WB
  • IP
  • IF
H M R Mk 25 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Endosomal Marker Antibody Sampler Kit provides an economical means of distinguishing endosomes in the early, late, and recycling phases. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody will detect endogenous total levels of their target protein. The antibodies do not cross-react with other isoforms, with the exception of the Rab11 (D4F5) XP® Rabbit mAb which will detect both Rab11a and Rab11b.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1, Pro1663 of human clathrin heavy chain protein, Ser70 of human EEA1 protein, Gly190 of human Rab5A protein, Glu188 of human Rab7 protein, and near the amino terminus of human Rab11 protein.

Background

Endosomes are formed by the invagination of the plasma membrane to form vesicles in an effort to recycle components of the cell (1). Endosomes can be coated in clathrin when vesicles form at clathrin-coated pits (2). Caveolins are 21-24 kDa integral proteins that interact with cholesterol and are the main structural components of the cholesterol/sphingolipid-enriched plasma membrane caveolae (3). Each stage of endosome maturation is marked by a unique set of proteins. EEA1 is an early endosome marker that is essential for membrane fusion and trafficking (4). Members of the ras superfamily of small Rab GTPases, specifically Rab5, Rab7, and Rab11 are markers of the early, late and recycling endosomes (5).

  1. Huotari, J. and Helenius, A. (2011) EMBO J 30, 3481-500.
  2. Rodriguez-Boulan, E. et al. (2005) Nat Rev Mol Cell Biol 6, 233-47.
  3. Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
  4. Christoforidis, S. et al. (1999) Nature 397, 621-5.
  5. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
For Research Use Only. Not For Use In Diagnostic Procedures.

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