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73977
CD38 (HIT2) Mouse mAb (APC Conjugate)
Antibody Conjugates

Monoclonal Antibody - CD38 (HIT2) Mouse mAb (APC Conjugate) - Flow Cytometry, UniProt ID P28907, Entrez ID 952 #73977

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Flow cytometric analysis of live human peripheral blood mononuclear cells using CD38 (HIT2) Mouse mAb (APC Conjugate) (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (APC Conjugate) #49083 (dashed line).

To Purchase # 73977S

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Isotype Mouse IgG1, kappa

Product Description

This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in human cells.

Product Usage Information

Application Dilutions
Flow Cytometry 1:20

Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

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Flow Cytometry

A. Solutions and Reagents

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
  2. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

B. Immunostaining

  1. Count cells using a hemacytometer or alternative method.
  2. Aliquot 0.5-1x106 cells into each assay tube (by volume).
  3. Add 2-3 ml Incubation Buffer to each tube and rinse by centrifugation. Repeat.
  4. Resuspend cells in 100 µl of diluted primary antibody (prepared in incubation buffer at the recommended dilution).
  5. Incubate for 1 hr at room temperature.
  6. Wash by centrifugation in 2-3 ml incubation buffer.
  7. Resuspend cells in the appropriate volume of PBS and analyze on flow cytometer.

posted June 2017

Protocol Id: 1504

Specificity / Sensitivity

CD38 (HIT2) Mouse mAb (APC Conjugate) recognizes endogenous levels of total CD38 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Human

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

Cyclic ADP-ribose hydrolase 1 (CD38) is a transmembrane protein involved in several important biological processes, including immune response, insulin secretion, and social behavior. Originally described as a glycosylated immune cell surface marker, additional research determined that CD38 is a multifunctional enzyme that catalyzes the synthesis and hydrolysis of cyclic ADP ribose (cADPR) from NAD (1,2). Under acidic conditions, CD38 also catalyzes the synthesis of nicotinic acid adenine dinucleotide phosphate (NAADP) from NADP+. Both cADPR and NAADP act as calcium ion mobilizing messengers that target different intracellular Ca2+ stores (3-6). Since CD38 is the primary mammalian NAD+ glycohydrolase responsible for NAD+ metabolism, CD38 may be a valuable therapeutic target for treatment of metabolic diseases regulated by NAD+-dependent pathways (7,8). CD38 has also been considered a possible therapeutic target for antibody-mediated therapy for myeloma and chronic lymphocytic leukemia (9-11).

  1. Malavasi, F. et al. (2008) Physiol Rev 88, 841-86.
  2. Jin, D. et al. (2007) Nature 446, 41-5.
  3. Lee, H.C. et al. (1999) Mol Cell Biochem 193, 89-98.
  4. Calcraft, P.J. et al. (2009) Nature 459, 596-600.
  5. Ogunbayo, O.A. et al. (2011) J Biol Chem 286, 9136-40.
  6. Lee, H.C. (2012) J Biol Chem 287, 31633-40.
  7. Cantó, C. et al. (2012) Cell Metab 15, 838-47.
  8. Escande, C. et al. (2013) Diabetes 62, 1084-93.
  9. Malavasi, F. et al. (2011) Blood 118, 3470-8.
  10. Deaglio, S. et al. (2010) Semin Cancer Biol 20, 416-23.
  11. Chillemi, A. et al. (2013) Mol Med 19, 99-108.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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