|M||Endogenous||Rat IgG2b, kappa|
Flow cytometric analysis of live mouse splenocytes using CD3 (17A2) Rat mAb (FITC Conjugate) (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (FITC Conjugate) #56722 (dashed line).Learn more about how we get our images.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
posted June 2017
Protocol Id: 1504
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test. A slight precipitate may be present, but will not interfere with the antibody performance. If precipitates are present, centrifuge the tube at 6,000xg for 10-30 sec. Draw off the supernatant and place into a light protective vial.
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
CD3 (17A2) Rat mAb (FITC Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. This antibody detects epitopes within the extracellular domains.
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.
When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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