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Product listing: Iodoacetamide, PTMScan® Qualified #88931 to FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #25243

Iodoacetamide is recommended for use in our PTMScan® protocols when making tissue or cell lysates just prior to the digestion steps.
Molecular Weight: 747.0 g/molPurity: >98%Molecular Formula: C40H67O11 • NaCAS: 28643-80-3 Solubility: Soluble in DMSO at 50 mg/ml and ethanol at 20 mg/ml.
$79
100 µg
This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.
APPLICATIONS
REACTIVITY
Mouse

Application Methods: Flow Cytometry

Background: Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

$79
100 µg
This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometry analysis in mouse cells.
APPLICATIONS
REACTIVITY
Human, Mouse

Application Methods: Flow Cytometry

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).

$79
100 µg
This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometry analysis in mouse cells.
APPLICATIONS
REACTIVITY
Mouse

Application Methods: Flow Cytometry

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).

$79
100 µg
This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.
APPLICATIONS
REACTIVITY
Mouse

Application Methods: Flow Cytometry

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).

Molecular Weight: 309.3 g/molPurity: >98%Molecular Formula: C15H15N7OCAS: 1224844-38-5 Solubility: Soluble in DMSO at 25 mg/ml.
LC-MS grade water (Burdick and Jackson™) is highly purified and recommended for use in HPLC, UPLC, and mass spectrophotometry applications (LC-MS) for low background contaminants.
Ammonium bicarbonate buffer is supplied as a one molar (1M) concentrated stock in HPLC grade water. This buffer is recommended for use in PTMScan protocols for LC-MS applications during the digestion procedures.
$63
125 ml
ELISA Wash Buffer (20X) is specifically formulated for use with both FastScan™ and PathScan® ELISA Kits. It is the recommended buffer to be used for all wash steps within the protocols for both kits.
$73
50 ml
This product is a concentrated fixation and permeabilization reagent designed for use with components of the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. This reagent immobilizes cellular proteins and enables antibody binding of transcription factors localized within the nucleus, and is compatible with fluorescent detection by flow cytometry.Note: Precipitation may occur. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

$71
150 ml
This product is a concentrated buffer suitable for antibody incubation and wash steps, and is designed for use with components of the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. This reagent enables antibody access to intracellular targets and is compatible with fluorescent detection by flow cytometry.Note: Precipitation may occur. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

$69
100 µg
This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.
APPLICATIONS
REACTIVITY
Human, Mouse

Application Methods: Flow Cytometry

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).

$69
100 µg
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in mouse cells.
APPLICATIONS
REACTIVITY
Mouse

Application Methods: Flow Cytometry

Background: Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

Molecular Weight: 277.7 g/molPurity: >98%Molecular Formula: C13H12CIN3O2CAS: 852391-15-2 Solubility: Soluble in DMSO at 25 mg/ml.
$59
100 µg
This Cell Signaling Technology antibody is conjugated to FITC and tested in-house for direct flow cytometric analysis in mouse cells.
APPLICATIONS
REACTIVITY
Mouse

Application Methods: Flow Cytometry

Background: Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

$59
125 ml
This product is a ready-to-use buffer, optimized for the dilution and incubation of both conjugated and unconjugated antibodies in flow cytometry assays (F). The buffer is formulated with 1X phosphate-buffered saline (PBS) compatible for use with live or fixed cells, and contains bovine serum albumin (BSA) to prevent antibody aggregation. Cell Signaling Technology recommends using this buffer according to our protocols for flow cytometry-approved antibodies to ensure accurate and reproducible results.
APPLICATIONS

Application Methods: Flow Cytometry

Urea is an organic compound that is highly soluble in water with strong protein denaturing properties. These properties make it ideal for use in preparation of Urea Lysis buffer for preparing cell and tissue extracts prior to PTMScan®-based applications. Urea, Ultrapure, PTMScan® Qualified from Cell Signaling Technology is offered in a convenient 54.05g size that allows for the preparation of 100ml of Urea Lysis Buffer with a final formulation of 20mM HEPES, 9M urea, and 1X Phosphatase Inhibitor Cocktail when following the Directions for Use.
$45
96 tests
The FastScan™ ELISA Microwell Strip Plate, 96 Well is a clear polystyrene plate that is coated with a proprietary antibody for use with colorimetric FastScan™ ELISA kits.Each 96-well plate is composed of 12 x 8-well strips/modules. Either the entire plate can be used at one time for an experiment, or it can be subdivided to use only the necessary number of 8-well strips.
$42
50 ml
FastScan™ ELISA Cell Extraction Buffer (5X) is used with FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #25243 (not supplied) to prepare and dilute cell extracts for use in FastScan™ ELISA Kits.
Molecular Weight: 447.5 g/molPurity: >98%Molecular Formula: C24H29N7O2CAS: 571190-30-2Solubility: Soluble in DMSO at 2 mg/ml.
$34
160 ml
This product is a diluent designed for use with components of the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. Proper dilution of the FoxP3/Transcription Factor Fixation/Permeabilization Concentrate (4X) #44931 results in the reagent immobilizing cellular proteins and enabling antibody binding of transcription factors localized within the nucleus. This product is compatible with fluorescent detection by flow cytometry.Note: Precipitation may occur. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

Trypsin is a serine endopeptidase derived from its inactive pancreatic zymogen, trypsinogen, when the N-terminal 6 amino acid leader sequence is enzymatically removed. Activated trypsin cleaves amide and ester bonds of lysine and arginine and this mass spectrophotometry grade Trypsin is intended for use in protein sequencing, and proteomics applications.
$21
5 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) is used with FastScan™ ELISA Cell Extraction Buffer (5X) #69905 (not supplied) to prepare and dilute cell extracts for use in FastScan™ ELISA Kits.