SignalStain® Antibody Diluent is for use in immunohistochemical assays. It yields superior staining with a number of antibodies. This diluent can improve IHC signal and background; however, it is not compatible with all of Cell Signaling Technology's products that are recommended for use in immunohistochemical assays. Please consult the antibody datasheet to determine if SignalStain® Antibody Diluent is recommended for your product of interest.
SignalStain® Citrate Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if citrate is recommended for your specific product.
SignalStain® EDTA Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if this solution is recommended for your specific product.
SignalStain® Mounting Medium is for use in immunohistochemical assays. This product permanently preserves tissue or cell preparations, and produces clear mounted sections, free from crystallization, with the ideal refractive index for high-resolution oil immersion microscopy. SignalStain® Mounting Medium retains the staining intensity of slides prepared with SignalStain® DAB Substrate Kit #8059.
The SimpleChIP® Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection.This product is provided in 1 ml volumes sufficient for preparation of 100 qPCR reactions, and is compatible with both enzymatic and sonication-fragmented DNA samples from SimpleChIP® enzymatic and sonication ChIP kits. This master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA, except primers and a DNA template.
All Species Expected
Background: Dye-based quantitative PCR (qPCR) uses real-time fluorescence of a double-stranded DNA (dsDNA) binding dye, most commonly SYBR® Green I, to measure DNA amplification as it occurs during each cycle of PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle, or CT value, can be determined. CT values can be used to evaluate relative target abundance between two or more samples, or to calculate absolute target quantities in reference to an appropriate standard curve, derived from a series of known dilutions. qPCR is commonly used to detect and quantify target genes in genomic DNA that is enriched by chromatin immunoprecipitation (ChIP).
Urea is an organic compound that is highly soluble in water with strong protein denaturing properties. These properties make it ideal for use in preparation of Urea Lysis buffer for preparing cell and tissue extracts prior to PTMScan®-based applications. Urea, Ultrapure, PTMScan® Qualified from Cell Signaling Technology is offered in a convenient 54.05g size that allows for the preparation of 100ml of Urea Lysis Buffer with a final formulation of 20mM HEPES, 9M urea, and 1X Phosphatase Inhibitor Cocktail when following the Directions for Use.
FastScan™ ELISA Cell Extraction Buffer (5X) is used with FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #25243 (not supplied) to prepare and dilute cell extracts for use in FastScan™ ELISA Kits.
This product is a ready-to-use buffer, optimized for the dilution and incubation of both conjugated and unconjugated antibodies in flow cytometry assays (F). The buffer is formulated with 1X phosphate-buffered saline (PBS) compatible for use with live or fixed cells, and contains bovine serum albumin (BSA) to prevent antibody aggregation. Cell Signaling Technology recommends using this buffer according to our protocols for flow cytometry-approved antibodies to ensure accurate and reproducible results.
This product is supplied as a 1X working solution for antibody dilution in immunofluorescence assays with cell cultures (IF-IC) or frozen tissue samples (IF-F). Cell Signaling Technology recommends using this buffer according to our protocols for IF-approved primary antibodies to ensure accurate and reproducible results. This product contains enough material for 500 assays based on a 100 μl assay volume.
This blocking reagent is designed to reduce noise originating from nonspecific protein-protein interactions in immunofluorescence assays. The core formulation includes goat serum as a protein blocker mixed with a mild detergent to facilitate permeabilization of cellular membranes.Cell Signaling Technology recommends using the buffer in accordance with our protocols for cultured cells (IF-IC) and frozen tissue sections (IF-F) to ensure accurate and reproducible results.The product is supplied as a 1X working solution and contains enough material for 500 assays based on a 100 μl assay volume.
Phosphate Buffered Saline (PBS-1X) pH 7.2 (Sterile) is specifically formulated to be used as a buffer for reconstituting sterile cytokines or antibodies. 1X PBS contains 10 mM Na2HPO4, 10 mM NaH2PO4, 150 mM NaCl.
Phosphate Buffered Saline (PBS) solution with the detergent Tween® 20 for use as a wash buffer and diluent for ELISA. 1 X PBST contains 3.2 mM Na2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, 0.05% Tween® 20, pH 7.4.
Tris buffered saline (TBS) solution for use as the blocking buffer diluent during fluorescent western blotting. Tween 20® detergent cannot be present in the blocking buffer because it can auto-fluoresce and increase non-specific background. After the blocking step, Tween 20® can be re-introduced to subsequent diluent buffers. Chemliluminescent western blotting does not require Tween 20® omission during blocking. Product is shipped and stored at room temperature.1X Formulation: 137 mM Sodium Chloride, 20 mM Tris. Supplied at pH 7.6.NOTE: This product does not contain Tween 20® detergent.
Tris buffered saline (TBS) solution with the detergent Tween® 20 for use as a wash buffer during western blotting. Product is shipped and stored at room temperature. 1X Formulation: 137 mM Sodium Chloride, 20 mM Tris, 0.1% Tween-20. Supplied at pH 7.6.
Tris-Glycine SDS Running Buffer (10X) is used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). Product is shipped and stored at room temperature. 1X formulation: 25 mM Tris, 192 mM Glycine, 0.1% SDS, pH 8.3.
Tris-Glycine Transfer Buffer (10X) is used as a transfer buffer during western blotting. Product is shipped and stored at room temperature.1X Formulation: 25 mM Tris, 192 mM Glycine, 20% (v/v) methanol, pH ~8.3.Note: Methanol is not supplied but is required.
10X Wash Buffer, Phosphate Buffered Saline (PBS) is for use in immunfluorescence and flow cytometry assays. Cell Signaling Technology recommends using this wash buffer according to our protocols for IF and FLOW approved antibodies to ensure accurate and reproducible results.1X Wash Buffer contains 137 mM NaCl, 2.6 mM KCl, 10 mM Na2HPO4, 1.76 mM KH2PO4, 0.005% sodium azide, pH 8.0. The product is free of calcium and magnesium salts.
The Cell Fractionation Kit is designed to provide a fast and efficient way of separating cultured cells into three distinct fractions: cytoplasmic, membrane/organelle, and nuclear/cytoskeletal. These fractions can then be analyzed by SDS-PAGE and western blotting. The kit includes enough buffer for 20 assays.
Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies (see Companion Products).
This kit provides the reagents needed to support antibody-based detection of FoxP3 and other transcription factors by flow cytometry. Optimally formulated fixation and permeabilization buffers halt biological activity and enable antibody access to intracellular targets. Antibody dilution, incubation, and wash steps are performed with the included FoxP3/Transcription Factor Permeabilization Buffer, eliminating the need for separate reagents.Note: Precipitation may occur in some kit components. The presence of precipitate does not affect the performance of the reagent.
The Immunofluorescence Application Solutions Kit is designed to conveniently provide the major supporting reagents needed for immunofluorescence in cell cultures (IF-IC) or frozen samples (IF-F). The reagents in this kit are thoroughly validated using our IF-approved antibodies and will perform optimally with the kit’s recommended protocol, ensuring accurate and reproducible results. This kit includes sufficient reagents for 100 assays based on a 100 µl assay volume.IMPORTANT: Please refer to the antibody data sheet to determine if it is validated and approved for use on cultured cell lines (IF-IC) or frozen tissues sections (IF-F) and for information regarding appropriate antibody dilution.Some primary antibodies may require methanol fixation or permeabilzation, which will be noted on the data sheet. Methanol is not included in this kit.