20% off purchase of 3 or more products* | Learn More >>

Flow Cytometry Buffer

$59
125 ml
This product is a ready-to-use buffer, optimized for the dilution and incubation of both conjugated and unconjugated antibodies in flow cytometry assays (F). The buffer is formulated with 1X phosphate-buffered saline (PBS) compatible for use with live or fixed cells, and contains bovine serum albumin (BSA) to prevent antibody aggregation. Cell Signaling Technology recommends using this buffer according to our protocols for flow cytometry-approved antibodies to ensure accurate and reproducible results.
APPLICATIONS

Application Methods: Flow Cytometry

$179
100 tests
1 Kit
The Intracellular Flow Cytometry Kit provides the supporting reagents needed to preserve protein states and enable antibodies to bind intracellular targets, for flow cytometric analysis of cells in suspension. This kit contains sufficient reagents for 100 individual samples when following the included protocol.IMPORTANT: Please refer to the antibody product page to determine if it is validated for use in Flow Cytometry (F) and for information regarding appropriate antibody dilution. Some primary antibodies may require detergent permeabilization, which will be noted on the datasheet. Detergent is not included in this kit.
APPLICATIONS

Application Methods: Flow Cytometry

$71
150 ml
This product is a concentrated buffer suitable for antibody incubation and wash steps, and is designed for use with components of the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. This reagent enables antibody access to intracellular targets and is compatible with fluorescent detection by flow cytometry.Note: Precipitation may occur. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

$140
1 Kit
This kit provides the reagents needed to support antibody-based detection of FoxP3 and other transcription factors by flow cytometry. Optimally formulated fixation and permeabilization buffers halt biological activity and enable antibody access to intracellular targets. Antibody dilution, incubation, and wash steps are performed with the included FoxP3/Transcription Factor Permeabilization Buffer, eliminating the need for separate reagents.Note: Precipitation may occur in some kit components. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

$34
160 ml
This product is a diluent designed for use with components of the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. Proper dilution of the FoxP3/Transcription Factor Fixation/Permeabilization Concentrate (4X) #44931 results in the reagent immobilizing cellular proteins and enabling antibody binding of transcription factors localized within the nucleus. This product is compatible with fluorescent detection by flow cytometry.Note: Precipitation may occur. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

$73
50 ml
This product is a concentrated fixation and permeabilization reagent designed for use with components of the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. This reagent immobilizes cellular proteins and enables antibody binding of transcription factors localized within the nucleus, and is compatible with fluorescent detection by flow cytometry.Note: Precipitation may occur. The presence of precipitate does not affect the performance of the reagent.
APPLICATIONS

Application Methods: Flow Cytometry

$73
5 x 10 ml
50 ml
Premium 16% (w/v) Formaldehyde from Cell Signaling Technology is used as a fixative agent for fluorescent immunocytochemical and flow cytometry assays. It is methanol-free, prepared from high quality paraformaldehyde, and packaged under an inert atmosphere of nitrogen.Each 10 ml solution is supplied in an amber glass vial with two access points, offering distinct advantages over pre-scored ampules. The screw cap allows for easy access to large volumes if necessary. To extend the product's shelf life, small volumes should be extracted by piercing the silicone top with a needle and syringe.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)

$109
5 x 20ml
100 ml
4% (w/v) Formaldehyde from Cell Signaling Technology is a ready-to-use fixative agent for fluorescent immunocytochemical and flow cytometry assays. It is formulated in 1X PBS, methanol-free, prepared from high-quality paraformaldehyde, and packaged under an inert atmosphere of nitrogen.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)