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Goat Secondary Antibody

$135
1 ml
Affinity purified goat anti-biotin antibody is conjugated to calf intestinal alkaline phosphatase. This product has been optimized for the detection of biotinylated protein molecular weight standards in western blotting applications.
APPLICATIONS

Application Methods: Western Blotting

Background: The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.

$135
1 ml
Affinity purified goat anti-biotin antibody is conjugated to horseradish peroxidase. This product has been optimized to detect biotinylated protein markers.
APPLICATIONS
REACTIVITY
All Species Expected

Application Methods: Western Blotting

Background: Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

$80
100 µl
$162
500 µl
Anti-rabbit IgG (H+L) was conjugated to DyLight™ 800 4X PEG fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 777 nm and peak fluorescence emission is 794 nm.

Background: Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

$80
100 µl
$162
500 µl
Anti-mouse IgG (H+L) was conjugated to DyLight™ 800 4X PEG fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 777 nm and peak fluorescence emission is 794 nm.

Background: Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

$203
250 µl
Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry

$203
250 µl
Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry

$142
250 µl
Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)

$142
250 µl
Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Immunofluorescence (Immunocytochemistry)

$142
250 µl
Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)

$142
250 µl
Anti-rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)

$121
250 µl
Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/mL.
APPLICATIONS

Application Methods: Immunofluorescence (Immunocytochemistry)

$142
1 ml
Affinity purified goat anti-rat IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.  This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.
APPLICATIONS
REACTIVITY
All Species Expected

Application Methods: Western Blotting

Background: Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

$142
1 ml
Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to biotin. This product has been optimized for use as a secondary antibody in western blotting applications.
APPLICATIONS

Application Methods: Western Blotting

$80
100 µl
$162
500 µl
Anti-rabbit IgG (H+L) was conjugated to DyLight™ 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm.

Background: Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

$80
100 µl
$162
500 µl
Anti-mouse IgG (H+L) was conjugated to DyLight™ 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm.

Background: Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

$39
1 ml
$367
15 ml
SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a ready-to-use solution intended for use in immunohistochemical assays to detect rabbit primary antibodies. SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all peroxidase-based substrates.
APPLICATIONS

Application Methods: Immunohistochemistry (Paraffin)

$142
250 µl
Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)

$199
250 µl
Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry

$199
250 µl
Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry

$142
250 µl
Anti-Mouse IgG (H+L) F(ab')2 Fragment antibody was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
APPLICATIONS

Application Methods: Flow Cytometry, Immunofluorescence (Immunocytochemistry)