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Human Thioredoxin-Disulfide Reductase Activity

Also showing Monkey Thioredoxin-Disulfide Reductase Activity

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse, Rat

Application Methods: Western Blotting

Background: TRXR1 (thioredoxin reductase 1) is a selenocysteine-containing protein that is involved in redox homeostasis (1-6). Its canonical target is thioredoxin, another redox protein (1). Together, they are involved in many functions such as antioxidant regulation (3-6), cell proliferation (2,3,5), DNA replication (2,3), and transcription (3,5). TRXR1 is also capable of reducing a wide array of cellular proteins (1,3). Selenium deficiency, either by diet modification (2,6) or introduction of methylmercury (4), hinders proper expression and function of TRXR1. It is possible that this effect, which results in a higher oxidative state, is a result of the selenocysteine codon (UGA) being read as a STOP codon in the absence of adequate selenium (4). The functions of TRXR1 in cell proliferation and antioxidant defense make it a potential therapeutic target.

$260
100 µl
APPLICATIONS
REACTIVITY
Hamster, Human, Monkey, Mouse, Rat

Application Methods: Immunohistochemistry (Paraffin), Western Blotting

Background: TRXR1 (thioredoxin reductase 1) is a selenocysteine-containing protein that is involved in redox homeostasis (1-6). Its canonical target is thioredoxin, another redox protein (1). Together, they are involved in many functions such as antioxidant regulation (3-6), cell proliferation (2,3,5), DNA replication (2,3), and transcription (3,5). TRXR1 is also capable of reducing a wide array of cellular proteins (1,3). Selenium deficiency, either by diet modification (2,6) or introduction of methylmercury (4), hinders proper expression and function of TRXR1. It is possible that this effect, which results in a higher oxidative state, is a result of the selenocysteine codon (UGA) being read as a STOP codon in the absence of adequate selenium (4). The functions of TRXR1 in cell proliferation and antioxidant defense make it a potential therapeutic target.

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey

Application Methods: Western Blotting

Background: Thioredoxin reductases are selenoproteins that are critical for maintaining cellular redox balance and eliminating reactive oxygen species (ROS) (1-4). There are three known mammalian thioredoxin reductases: TRXR1/TXNRD1 (cytosolic), TRXR2/TXNRD2 (mitochondrial), and TRXR3/TXNRD3 (testis-specific) (1). TRXR2 may function to protect against tumor necrosis factor-α (TNF-α)-mediated ROS generation (5). TRXR2 is critical for normal heart development and function (1,4). TRXR2 KO mice experience embryonic lethality, while mitochondrial dysfunction seen in heart-specific TRXR2 KO mice results in congestive heart failure (4). In humans, certain rare mutations in TRXR2 have been linked to dilated cardiomyopathy (1). A recent study in the EPIC-Heidelberg cohort shows that serum selenium levels, in concert with certain SNPs in TRXR2 and other selenoproteins, can influence prostate cancer risk (6).