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Monoclonal Antibody Protein Phosphatase Type 1 Complex

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Mouse, Rat

Application Methods: Immunofluorescence (Immunocytochemistry), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting

Background: Spinophilin is an 815 amino acid protein composed of a PDZ domain, 2 actin-binding domains, a receptor- and PP1-binding domain, three coiled-coiled domains, a potential leucine/isoleucine zipper motif, and three potential SH3 domains (1). Spinophilin interacts with a large number of proteins including ion channel components and G protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (2). Spinophilin has been shown to affect GPCR function through two different mechanisms: spinophilin acts as a functional inhibitor of α-2 adrenergic receptor-mediated arrestin signaling by competing with GRK2 binding to the adrenergic receptor (3) and spinophilin facilitates μ-opioid receptor desensitization by promoting receptor endocytosis (4).

$260
100 µl
APPLICATIONS
REACTIVITY
Hamster, Human, Monkey, Mouse, Rat

Application Methods: Western Blotting

Background: NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse, Rat

Application Methods: Western Blotting

Background: SHOC2 is a scaffolding protein that harbors multiple leucine-rich repeats in tandem and is an upstream positive regulator of growth factor-dependent MAPK/ERK signaling. Research studies have demonstrated that SHOC2 forms a complex with the catalytic subunit of the PP1 phosphatase and M-Ras, and this complex drives activation of Raf-ERK signaling in response to mitogenic growth factors (1). SHOC2 has also been shown to cross-talk with and activate the PI3K/Akt signaling axis through its interaction with the p110α catalytic subunit of PI3K (2). As a positive regulator of ERK and PI3K/Akt signaling cascades, SHOC2 has been implicated in the regulation of several oncogenic cellular processes such as cell motility, invasion, and metastasis (2). A mutation in SHOC2 that introduces an N-terminal myristoylation site, promotes aberrant membrane targeting of SHOC2, hyperactive MAPK/ERK signaling, and Noonan-like syndrome (3).

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse, Rat

Application Methods: Immunoprecipitation, Western Blotting

Background: NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).