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Polyclonal Antibody Bicarbonate Transport

Also showing Polyclonal Antibody Immunoprecipitation Bicarbonate Transport, Polyclonal Antibody Western Blotting Bicarbonate Transport

$260
100 µl
APPLICATIONS
REACTIVITY
Human, Monkey, Mouse

Application Methods: Immunoprecipitation, Western Blotting

Background: NBC1/SLC4A4 is an eletrogenic sodium bicarbonate cotransporter. It mediates the coupled movement of Na+ and HCO3- ions across the basolateral membrane of cells. NBC1 regulates secretion and absorption of bicarbonate and modulates intracellular pH (1-3). There are five isoforms of NBC1 produced by alternative splicing including the most studied isoforms, 1 and 2, which have different N termini. The N terminus of isoform 1 contains multiple consensus phosphorylation sites for various kinases, such as PKA, PKC, and CK II, and may play a regulatory role in the biological function of NBC1 (4). Interaction with carbonic anhydrase II, IV, and IX regulates the transporter acitivity of NBC1 (5-7). NBC1 is expressed in various tissues with the exception of isoform 2, which is mainly expressed in kidney proximal tubules (1). Isoform 1 is expressed in the pancreas and corneal endothelium, and at low levels in other tissues including heart, skeletal muscle, liver, and prostate (4). Research studies have shown that mutations in the NBC1 gene are linked to proximal renal tubular acidosis with ocular abnormalities (also known as renal tubular acidosis II).

$260
100 µl
APPLICATIONS
REACTIVITY
Human

Application Methods: Immunoprecipitation, Western Blotting

Background: Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).