New SimpleChIP® Assay Kits from Cell Signaling Technology combine several ChIP validated antibodies with control PCR primer mixes that serve as markers for pluripotency or epigenetic status. Our rigorous quality control and in-house testing ensure the antibodies included in the kits meet the highest standards for quality, validation, and lot-to-lot consistency. The kits provide all reagents necessary to perform 10 ChIP assays and subsequent real-time PCR reactions. Pre-selected positive and negative primer sets are included in each kit, providing proven and appropriate controls for customer experiments.
SimpleChIP® Stem Cell Master Regulator Assay Kit #8980: NTERA-2 cells were either untreated (A) or treated for 15 days with retinoic acid (RA) (B) to induce differentiation along the neuronal lineage (bottom panel). Chromatin IPs were then performed with cross-linked chromatin from 4 x 106 cells and 10 μl of Nanog, Oct-4, and Sox2 antibodies, or 2 μl of Normal Rabbit IgG, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Note the loss of Nanog, Oct-4, and Sox2 binding to target genes as NTERA-2 cells are induced to differentiate.
|8982||SimpleChIP® Human Bivalent Promoter Assay Kit||ChIP||H|
|8981||SimpleChIP® Mouse Bivalent Promoter Assay Kit||ChIP||M|
|8980||SimpleChIP® Stem Cell Master Regulator Assay Kit||ChIP||H|
Applications Key: ChIP=Chromatin Immunoprecipitation
Reactivity Key: H=Human M=Mouse