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Information and case study data for the PTMScan Direct Apoptosis/Autophagy Service offered by CST.
Reliably identify senescence signaling in your samples using biomarkers with a 3-stage approach outlined in this video. No single assay is sufficient, but screening, co-staining, and verification with multiple biomarkers can improve your confidence.
For PTMScan experiments, the stage tip, or small-scale peptide desalting is a critical part of the experiment for a number of reasons. Detailed instructions for performing stage tip peptide desalting are included at the end of each PTMScan kit protocol.The stage tip step will remove salts and other contaminants prior to mass spectrometry that can distract the mass spectrometer from peptide identification. Importantly, this step will also serve to reduce the amount of antibody that may co-elute with the peptides and contaminate the analytical column or LC. This will improve the longevity of the analytical column and lead to more reproducible data.Stage tips can be made using an 18 gauge needle and C-18 disks, according to the method published by Rappsilber et al (Anal. Chem. 2003, 75, 3, 663–670, 2002). Alternatively, the tips can be purchased from a number of suppliers.The key to a successful desalting step is using a high-capacity stage tip, or one that is suitable for ~10ug of…
Drug discovery and development can incorporate proteomics studies which enable accelerated target identification and deeper insights of mechanism of action and off-target effects.
There are no tools or therapies to cure, prevent, or slow the progression of AD. Current therapeutic approaches include targeting amyloid plaques and neurofibrillary tangles.
Specific Aims set the stage for your grant proposal and are often considered the most important part, so make sure you're concisely conveying your goals.
B cells are lymphocytes that are an important part of the humoral immune response. The main role of B cells is to produce antibodies in response to antigens.
Researchers are still working hard to develop effective treatments for SARS-CoV-2 and COVID-19.
Products and Related Resources for Type I Interferon and Viral Restriction Factors SARS-CoV-2 Research
Partnerships & Licensing information
Discover the key RNA binding proteins (RBPs) in the lifecycle of messenger RNA (mRNA)
Overview of stem cell markers, lineage markers, development and differentiation signaling networks at Cell Signaling Technology.
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
Each summer, college and high school interns at CST get a chance to sample work life in a biotech environment. Students at this stage are typically considering potential career paths, so what better time to set up a Q&A panel? Five CST employees shared some of their career development experiences.
Unique solutions for drug discovery and development—custom antibody conjugations and formulations, bulk orders, ELISA kits, and proteomics services.
Products and Related Resources for Cytokines and Inflammation SARS-CoV-2 Research
PTMScan Discovery in Translational Research (A Case Study)
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
Roughly 1 in 8 men will be diagnosed with prostate cancer. So why do so many men lose their battle? Emerging therapeutic options may turn this around.
Feb 2008, CST continued collaboration with AstraZeneca to provide CST’s PhosphoScan® Platform for kinase inhibitor proteomic profiling.
We’ve put together a starter’s guide on the cellular mechanisms that drive neurodegeneration in diseases such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis, and Huntington’s disease.
The background or number of non-PTM peptides in each enrichment is primarily due to non-specific interactions with the beads the antibody is conjugated to. Therefore, this background is also non-depletable by methods such as pre-clearing.The level of background peptides can range considerably depending on the PTM and the biological source. PTMs that are typically lower in abundance (e.g., phosphotyrosine) can be expected to have a higher background compared to more abundant PTMs like the ubiquitin remnant (or K-e-GG) and acetyl lysine. Peptides derived from cells in culture typically produce more PTM peptides than an equivalent amount of protein from tissue.A major source of background peptides can be carry-over of antibody-beads to the stage tip or small-scale desalting at the end of the protocol. For optimal results, the eluted peptides should be clear of any antibody-beads before loading onto the stage tip. The pH of the peptides in the IAP enrichment step may also affect the specificity…
Therapeutic developments in treating Alzheimer’s, novel diagnostic tools, and new insights into neurodegeneration mechanisms were key topics at AD/PD 2023.
This guide includes some of the most common markers used to detect neuronal and glial cells, which include oligodendrocytes, astrocytes, and microglia.
We understand some customers choose to pursue TMT labeling for PTMScan proteomics analysis, but we do not have a defined protocol for this method. However, there are two questions we can address:
1) We know that labs have succeeded in performing this experiment by enriching for the PTM peptides first then labeling second. This is critical for UbiScan and ubiquitin remant in particular because the TMT label is now on the K-e-GG remnant and the antibody won't be able to bind if the labeling is performed first.
2) We have recently found that to recover the TMT peptides efficiently from the stage tip C-18 desalting step you need to use 50% ACN/ 0.15% TFA (an increase from 40%). The TMT label makes the peptides more hydrophobic and the increase in ACN helps elute them from the C-18.
A scientific resource for the GLUE protein domain containing information on structure, function, and binding to phospholipids in the regulation of vesicle trafficking.
Navigating peer review can be challenging for early-career researchers. Here are tips for how to choose peer reviewers for your suggested reviewer list.
How CST responds to customer feedback as it relates to development of the product portfolio.
Drs. Michael Keebler and Chaolin Zhang present work on protein-RNA dynamics in neuronal synapses and RNA splice regulation in neuronal development.
July 2013, CST awarded patents critical to lung cancer therapy. Secures dominant IP position for EML4-ALK in NSCLC that spans from research through diagnosis and therapy.
