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Resources results for "phospho-ikk%25CE%25B1"

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How Do I Measure Metabolism?

Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.

Protein and Pathway Abbreviations

Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.

PTB Protein Domain

A scientific resource for the PTB protein domain containing information on structure, function, and domain binding to phospho-tyrosine motifs.

Virus Detection and Innate Immune Activation

Products and Related Resources for Virus Detection and Innate Immune Activation SARS-CoV-2 Research

NF-κB Signaling

Explore the NFkB pathway and its impact on inflammation and immune responses. Click here to learn more about this signaling pathway.

Death Receptor Signaling

Expert-reviewed interactive pathway providing a current overview of Death Receptor Signaling.

Toll-like Receptor Signaling

Expert-reviewed interactive pathway providing a current overview of TLR signaling.

Immunology: What are toll-like receptors and how do they invoke tumor tolerance?

Understanding how TLR signaling is activated by molecular patterns is relevant not only to their role in combating infection but also in cancer.

Cytokines and Inflammation

Products and Related Resources for Cytokines and Inflammation SARS-CoV-2 Research

Solutions for Oncology Therapeutics Discovery

Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.

Matched Antibody Pairs

Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.

Altered Metabolism

The three main purposes of metabolism are the conversion of food to energy; the conversion of nutrients to proteins, carbohydrates, lipids, and nucleic acids; and the elimination of nitrogenous wastes. There is a strong correlation between metabolic chan

Type I Interferon and Viral Restriction Factors

Products and Related Resources for Type I Interferon and Viral Restriction Factors SARS-CoV-2 Research

Motif Antibodies for PTMScan® Discovery Services

PTMScan Motif Antibodies and Kits, for customers who purchase a license and would like to perform PTMScan Proteomics in-house.

PTMScan® Motif Antibody Kits

PTMScan Motif Antibody Kits from Cell Signaling Technology

Adaptive Immune Response

Products and Related Resources for Adaptive Immune Response SARS-CoV-2 Research

Altered Cell Signaling

Abnormal cell-cell communication, for example disrupted presynaptic input, as well as disrupted intracellular signaling contribute to the pathogenesis of neurodegenerative disease.

Protein Folding and Aggregation

We’ve put together a starter’s guide on the cellular mechanisms that drive neurodegeneration in diseases such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis, and Huntington’s disease.

Resources

Phospho-GSK-3β antibodies that detect phosphorylation at Ser9 upon activation in properly treated samples are also capable of detecting the GSK-3β-Tag. Although these antibodies are specific to the Ser9 site of GSK-3β, the GSK-3β-Tag is quite similar to their antigen sequences.  

CST offers the following phospho-GSK-3β (Ser9) antibodies which also detect the GSK-3β-Tag: 
Phospho-GSK-3β (Ser9)(D85E12) XP® Rabbit mAb #5558
Phospho-GSK-3β (Ser9)(5B3) Rabbit mAb #9323
Phospho-GSK-3β (Ser9)(D2Y9Y) Mouse mAb #14630
Phospho-GSK-3β (Ser9) Antibody) #9336

Akt Antibody Comparison Tables

Compare Cell Signaling Technology's Akt antibodies against one another to determine optimal applications

Resources

Our Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 detects over-expressed levels of PKCalpha and beta. It also detects endogenous levels of PKC in Jurkat + CalA extract and possibly weakly in other endogenous models tested. Our Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106 and Phospho-Akt (Thr308) Antibody #9275 detect over-expressed levels of PKCalpha and beta, but do not detect endogenous PKC. Our Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 detects over-expressed levels of PKCalpha and beta and may also weakly detect endogenous PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Thr308) (D25E6) XP Rabbit mAb #13038 does not detect over-expressed PKC and is the best choice of the Phospho-Akt (Thr308) antibodies if this is a concern.

Resources

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338, and Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 are performance tested on extracts prepared from serum-starved cells that have been treated with 10ng/ml TGF-beta3 for 30 minutes.  It is possible that longer treatments (e.g., 24 hours) can lead to diminished signals for phospho-Smad2 and phospho-Smad3 because of Smad7 induction. 

​​Failure to include the appropriate serine/threonine phosphatase inhibitors can result in a diminished signal and/or inconsistent results between experiments. We always include sodium pyrophosphate (2.5mM final) and beta-glycerophosphate (1.0mM final) as serine/threonine phosphatase inhibitors in the lysis buffer for detection of the phospho-Smads. Our Phosphatase Inhibit…

Fibrosis

Products and Related Resources for Fibrosis SARS-CoV-2 Research

Cell Death and Viability

Products and Related Resources for Cell Death and Viability SARS-CoV-2 Research

Stability Test Results

As a new antibody is developed, Cell Signaling Technology scientists test the antibody’s stability and activity

FHA Protein Domain

A scientific resource for the FHA protein domain containing information on structure, function, and binding to phospho-serine and phospho-threonine motifs.

Resources

Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 and Phospho-Akt (Ser473) (D9W9U) Mouse mAb #12694 detect over-expressed PKC alpha and beta. Our Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 also detects endogenous levels of PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 detects over-expressed PKC alpha and beta but does not detect endogenous PKC in the models tested. Our Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 does not detect over-expressed or endogenous PKC alpha and beta.

Resources

Phosphorylation of serines 465 and 467 indicates Smad2 activation and nuclear localization; whereas, phosphorylation of serines 245, 250, and 255 is inhibitory and leads to retention of Smad2 in the cytoplasm.

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108, Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb #8828, and Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb #18338 recognize phosphosites at the carboxy-terminus of Smad2 that are phosphorylated in response to TGF-beta treatment. Phospho-Smad2 (Ser245/250/255) Antibody #3104 instead recognizes a cluster of phosphosites in the Smad2 linker region that are phosphorylated by MAP kinase.  
 

Erk Antibody Comparison Table

Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications

Mastering Phospho-Flow: Immune Cell Activation Webinar

Mastering Phospho-Flow: Immune Cell Activation Webinar