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Products and Related Resources for Type I Interferon and Viral Restriction Factors SARS-CoV-2 Research
Products and Related Resources for Virus Detection and Innate Immune Activation SARS-CoV-2 Research
How does the choice of blocking protein—nonfat dry milk vs bovine serum albumen (BSA)—affect your western blot (WB) results? Watch the Tech Tips video.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
A scientific resource for the BRCT protein domain containing information on structure, function, and binding to phospho-serine motifs.
Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.
A scientific resource for the MH2 protein domain containing information on structure, function, and domain binding to phospho-serine motifs during TGF-beta signaling.
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
The accuracy of western blot results relies heavily on the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.
Catalog numbers in tabular format for control cell extracts and proteins with links to product pages.
Products and Related Resources for Viral Entry, Replication, and Transcription SARS-CoV-2 Research
Expert scientist-authored white paper focused on phosphorylation-dependent protein interaction domains in signal transduction.
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
Breakthroughs in therapeutic targeting of YAP/TAZ-TEAD signaling using small molecules were presented at AACR 2023. Read a recap of the presentations.
Reggie Prioli, Associate Director of Conjugation at CST®, has been conjugating antibodies at CST since 2001. Let’s find out why!
Quantitative analysis of the SMAD signaling pathway using ELISA, high content imaging, and high-resolution confocal approaches were used to study the regulation of angiogenesis and cell proliferation.
It is possible to see a doublet with our Phospho-c-Jun (Ser73) (D47G9) XP Rabbit mAb #3270 in some extracts. This is because #3230 can recognize JunD phosphorylated at Ser100. Phospho-c-Jun (Ser73) is the higher band at approximately 48 kDa, whereas phospho-JunD (Ser100) is the lower band at approximately 42 kDa.
CST Technical Support Article
Zika virus turns off Akt signaling to hijack autophagy in developing neural tissue
CUT&RUN Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
New antibodies for Alzheimer's research were presented at SfN 2022, including tools to study neuroinflammation in glial cells like microglia and astrocytes
A scientific resource for the WD40 protein domain containing information on structure, function, and binding to both phospho-Ser/Thr and methylated lysine residues.
If our recommended buffers are being used for western blotting and high background or additional bands persist when probing for phospho-AMPK (Thr172), the issue could be related to the lysate sample. In general, tissue samples can be prone to high background by western blotting when compared to cultured cell lines, and liver tissue samples (a high expressing model for AMPK) in particular tend to show high background in the form of multiple bands when probing for phospho-AMPK. Sonication and the use of proper buffers for western blotting can help to alleviate this issue in some instances.
"PI3K/Akt signaling is close to my heart because certain diseases that have cropped up in my family are linked to specific proteins in this pathway."
Alphabetical listing of protein, pathway, and antibody acronyms, curated by Cell Signaling Technology.
It is critical that our Phospho-p53 (Ser15) Antibody #9284 be diluted into 5% w/v non-fat dry milk, 1X TBS, 0.1% Tween® 20 and incubated at 4°C with gentle shaking, overnight. Diluting the antibody in BSA will result in high background on the blot.
List of publications based on primary research done by the Cancer Research Group at CST (2002-2013).
CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
Activated by pro-inflammatory signaling, ischemic injury or viral infection, necroptosis is a programmed regulated form of necrosis requiring RIP3 and MLKL
Cellular homeostasis is regulated by several key metabolic pathways that maintain energetic status and provide the building blocks for proper function.