There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
Metabolism defines all of the chemical changes or processes within cells, tissues, and organisms that sustain cellular homeostasis.
Modulation of CNS function occurs through synaptic neurotransmission, which is the signaling from the axon terminal of one neuron to the dendrites of another via molecules called neurotransmitters.
Neuroinflammation is the activation of an immune response in the CNS by the microglia and astrocytes. While not linked mechanistically to neurodegenerative diseases, neuroinflammation is associated with the progression of Alzheimer's disease, Parkinson's
Neurodegeneration refers to disorders of the CNS that lead to loss of neurons or glia, and is associated with age-related diseases, autoimmune attacks on the nervous system, or genetic mutations that affect the heath of CNS cells.
Cell viability is a measure of the proportion of live, healthy cells within a population. Assays to assess cell viability measure metabolic activity, ATP content, cell proliferation, or membrane integrity.
Tau is a microtubule-associated protein found in neurons, and its function is to stabilize axonal microtubules. Hyperphosphorylated tau is a hallmark of several neurodegenerative diseases including Alzheimer’s disease.
Troubleshooting tips for successfully performing Intracellular Flow Cytometry using CST recommended protocols and antibodies validated for Flow.
RNA Regulation, Epitranscriptomics, and Disease | Cell Signaling Technology
Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.
A broad range of techniques are used to study neurodegenerative diseases including basic cellular and molecular protocols, ELISA, electrophysiological assessments, and imaging-based methods that utilize antibody-based technology.
Case Study 4: Exceptional Performance of XP Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Discover how to successfully stain tissue with antibodies for immunohistochemistry (IHC) experiments. See examples of successfully stained IHC assays.
Learn how to visualize protein expression in tissue with immunohistochemistry (IHC). See examples of successfully optimized IHC assays.
In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.