Basket Updated
0
Items added
Highly specific and sensitive rabbit mAbs against B7 family targets, B7-H3 & B7-H4, are proven to outperform existing clones by WB.
CST offers a growing portfolio of products for the study of immune checkpoint targets in cancer, including several receptor-ligand pairs.
Why not take advantage of our own immune system and the best of its weaponry, the T-cells, to fight cancer? This is the basis of immunotherapy.
Cell Signaling Technology Announces the Release of a First to Market IHC-Approved VISTA Antibody
Monoclonal antibodies developed and validated by CST can enable co-detection and spatial characterization of important immune checkpoint control proteins using mIHC
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
PD-L1/PD1 immune checkpoint helps tumor cells evade detection by the immune system. Highly specific and sensitive PD-L1 antibody now available from CST.
The predicted molecular weight of B7-H4 is 28.8 kDa, but by western blot it is usually observed at 40-80 kDa. This is due to glycosylation of the protein. It is common for a target to shift to higher molecular weights with post-translational modification (e.g., glycosylation). Please see Salceda, S. (2005) et al. Exp Cell Res. 306, 128-41 (PMID 15878339; http://www.ncbi.nlm.nih.gov/pubmed/15878339).
A scientific resource for the Chromo protein domain containing information on structure, function, and binding to methylated lysine residues during chromatin remodeling.
Histone modification antibodies are validated using a peptide array assay at CST
A scientific resource for the TUDOR protein domain containing information on structure, function, and domain binding to methylated-lysine residues in chromatin remodeling.
Interact with this diagram showing the epigenetic regulators of Histone H3 including those responsible for monomethylation, asymmetric and symmetric dimethylation, and trimethylation.
Interact with this signaling pathway showing immune checkpoint regulation in the tumor microenvironment, including targets such as PD-L1, GITR, TIM-3, CTLA-4, OX40, and more.
Cell viability assays measure the population of live, viable cells within a sample. Typically, viability assays measure markers of cell health, including cellular metabolism, ATP levels, and cell proliferation.
Resources include signaling pathways, antibodies and companion products, research overviews, technical resources, and recent review articles.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Discover the most important epigenetic markers that contribute to the progression of diffuse intrinsic pontine glioma (DIPG).
The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.
Learn how to visualize protein expression in tissue with immunohistochemistry (IHC). See examples of successfully optimized IHC assays.
Find Pathways and Diagrams By Disease Area
We see this doublet phenomenon often in a number of different cell lines that we test with our Histone H3 antibodies. It does appear to be sample dependent. The doublet may be due to a proteolytic fragment of Histone H3, either a cleavage product of histone H3 at the N-terminus, or a protein degradation fragment.
A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.
Hallmarks of Antibody Validation-Complementary Strategies that Employ Western Blot, Immunohistochemistry (IHC), Immunofluorescence (IF/ICC), Flow Cytometry, Histone Antibody, ChIP-qPCR, ChIP-seq.
CUT&Tag Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
When it comes to mouse tissue samples, not all loading control proteins are expressed equally. Learn which control is right for your research.
This is a four-part series of tips to improve your ChIP protocol.
ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment.
Find out which epigenetic markers are important drivers in mixd lineage leukemia, or MLL.
