Basket Updated
0
Items added
Epigenetic regulation, including aberrant DNA methylation and histone modifications, have been linked to Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, multiple sclerosis, and amyotrophic lateral sclerosis.
Discover the epigenetic drivers that help drive MDS / AML cancers
Theoretically, our 5-Methylcytosine (5-mC) (D3S2Z) Rabbit mAb #28692 should detect the RNA m5C modification but we have not specifically tested this in-house.
Click here to discover the intricacies of methylation pathways and their role in gene regulation and epigenetic modifications.
If a dot blotting apparatus is not available for use, we recommend denaturing your DNA in a lower volume/higher concentration (see modified steps below):Section B. Dot Blot Modified Steps:1. Dilute fragmented genomic DNA to 400 ng/ul in 100 ul of nuclease-free water. Then denature DNA by adding 100 ul of 2X DNA Denaturing Buffer and incubating at 95C for 10 min.2. Add 200 ul of 20X SSC buffer and immediately chill on ice for 5 min. DNA concentration of the solution is 100 ng/ul.3. Set up a series of six 2-fold dilutions by adding 200 ul of the DNA solution, starting with the DNA solution in Step 2, to 200 ul of nuclease-free water. This will generate seven DNA samples containing 200 ul DNA at concentrations of 100 ng/ul, 50 ng/ul, 25 ng/ul, 12.5 ng/ul, 6.25 ng/ul, 3.125 ng/ul and 1.563 ng/ul.4. Spot 10 ul of each of the seven dilution samples onto the nylon membrane, leaving the last well for nuclease-free water only. The amount of DNA added to each well should then be 1000 ng, 500 ng, …
Cell Signaling Technology events including conference, vendor show and event listings.
List of publications based on primary research done by the Cancer Research Group at CST (2002-2013).
Hallmarks of Antibody Validation: Ranged Strategy. Binary models are not always readily available and can be time-consuming to produce for validating an antibody.
A ranged validation strategy includes both endogenous and heterologous models that express high, moderate, and low levels of the target of interest.
These antibodies target a post-translational modification (PTM) on DNA rather than protein. In order to adequately expose the epitope for binding, the DNA needs to be partially denatured. To accomplish this, we use ethanol and HCl. Traditional formaldehyde fixation followed by detergent-based permeabilization methods will not work for these PTMs.
Interested in studying senescence? Understanding cell cycle arrest is critical to many fields of research, including development, aging, and cancer.
Explore the ERK pathway and its role in cell proliferation and survival. Click here to read more about this critical signaling pathway.
The Jak/Stat Utilization Table displays the combinatorial use of tyrosine kinases and Stat proteins in cytokine/growth factor signaling.
A comprehensive list of peer-reviewed publications from Cell Signaling Technology.
The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).
The immune system is composed of tissues, cells, and molecules whose primary function is to detect, respond to, and eliminate pathogens and transformed cells.
Changing the primary antibody dilution and incubation conditions affects fluorescence intensity and signal-to-noise ratio in IF experiments.
Cell Signaling Technology, Inc. Announces License in Personalized Cancer Diagnostics.
CST Media Room
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Autophagy helps normal cells maintain homeostasis and acts as a survival mechanism in response to stress, where ULK1 plays an important role in signaling.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Dec 2011, CST wins "Best Performing Antibodies" and "Best Breakthrough Products Cancer Research" Categories in Life Science Industry Awards.
Usually, the CUT&Tag DNA libraries from histone targets have a higher concentration than those from non-histone targets. We use the following formula to convert a library concentration from ng/µL to nM before diluting each library sample to the same concentration (nM) for pooling purposes: Concentration (nM) = 1,000,000 X Concentration (ng/µL) / library average size (bp) / 660. In addition to obtaining a greater library size for undetectable library samples from the Bioanalyzer or TapeStation system (as described in the above question), we would also suggest pooling the libraries that have a flat signal of 5-10 fold more than the libraries that show normal sized peaks on the Bioanalyzer or TapeStation systems. This ensures an even distribution of the number of reads among all samples. Usually, a library pool concentration of 2 nM DNA is enough for NGS purposes, although a higher concentration is always welcome.
Listing of IHC-related scientific posters and papers involving scientists from Cell Signaling Technology.
Interact with this CAR-T signaling pathway to learn how the combination of extracellular and intracellular domains, including CD8, CD28, 4-1BB, OX40, or CD27, exert antitumor effects.
Expert-reviewed interactive pathway providing a current overview of Hedgehog Signaling.
