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The antigens for our COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 are 100% conserved with COX IV isoform 1. There is less than 20% homology of these antigens with COX IV isoform 2, therefore we do not expect these antibodies to detect isoform 2.The antigen for our COX IV (4D11-B3-E8) Mouse mAb #11967 is also 100% conserved with COX IV isoform 1. However, the antigen used to produce #11967 is 79% homologous with COX IV isoform 2. Therefore there may be cross-reactivity of this antibody with isoform 2, but we have not tested this in-house and cannot guarantee it.
When it comes to mouse tissue samples, not all loading control proteins are expressed equally. Learn which control is right for your research.
Our COX IV (3E11) Rabbit mAb #4850 will specifically label human cells and not mouse, and our COX IV (D6I4K) Rabbit mAb (Rodent Specific) #38563 will specifically label mouse cells and not human.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
Loading controls are essential to ensure data reliability in your western blot. Learn about the three main considerations for loading control selection.
Ensure equal sample loading with loading control antibodies from CST.
These antibodies to CNS markers can help study how LRRK2 mutations impact the autophagic-lysosomal pathway (ALP) and contribute to Parkinson's disease.
In response to a variety of environmental factors, cells may permanently cease proliferation and enter a state known as cellular senescence.
There are many reasons to include a subcellular marker in your IF experiment: studying organelle-specific functions, assessing drug effects, and more.
Metabolism defines all of the chemical changes or processes within cells, tissues, and organisms that sustain cellular homeostasis.
Unravel the HER2 signaling pathway and its role in cell growth and cancer. Explore the molecular mechanisms and signaling events. Click here.
In the CUT&RUN kit protocol, the addition of digitonin to the buffers facilitates the permeabilization of cell membranes & the entry of the primary antibody & pAG-MNase enzyme into the cells & nuclei.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
A scientific resource for the BRCT protein domain containing information on structure, function, and binding to phospho-serine motifs.
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
Learn DNA library preparation for ChIP-seq and CUT&RUN using the Illumina® protocol, from end repair & adaptor ligation to PCR enrichment & cleanup.
Listing of IHC-related scientific posters and papers involving scientists from Cell Signaling Technology.
Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.
The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).
Fostering mentorship, application-based learning, and a love of science are all part of the mission of the CST internship program. Students interning with us are partnered with CST Mentors.
Multiplex Oligos for Illumina Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.
Multiplex Oligos for Illumina Protocol (Single Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.a
A troubleshooting guide for CUT&Tag–a ChIP-seq alternative with lower sample requirements, lower sequencing cost, and improved signal-to-noise for profiling chromatin and protein-DNA interactions.
Expert-reviewed interactive diagram of Rheumatoid Arthritis Pathogenesis and relevant antibodies for studying targets associated with the autoimmune disease.
Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.
Step-by-step ChIP assay protocol (agarose beads). A powerful technique used to study protein-DNA interactions, identify regulatory elements. Learn more.
SimpleChIP Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.