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Resources results for "n6-methyladenosine %28m6a%29"

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Hallmarks of Antibody Validation: Complementary Strategies

Hallmarks of Antibody Validation-Complementary Strategies that Employ Western Blot, Immunohistochemistry (IHC), Immunofluorescence (IF/ICC), Flow Cytometry, Histone Antibody, ChIP-qPCR, ChIP-seq.

Hallmarks of Validation - Complementary Strategies

Complementary strategies provide vital information regarding antibody specificity or functionality and can be tailored to the nature of the downstream assay.

Understanding the Role of RNA Regulation and Epitranscriptomics in Disease

Learn how RNA-binding proteins influence RNA regulation and epitranscriptomics and contribute to the progression of a variety of diseases.

m6A RNA Regulatory Diagram

Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development

M6A: A Cell’s Hidden Signal of Gene Regulation

There is still much to learn about the hidden layer of gene regulation—the chemical modification of RNA. What is the effect of m6A, the most abundant mRNA modification observed in eukaryotes?

tRNA Modification by m7G MTase: Understanding its Role in Cancer and Developmental Disorders

Guest blogger Dr Gregory discusses research to define the epitranscriptome, including modifications that increase tRNA stability and prevent degradation.

Conferences & Events

Cell Signaling Technology events including conference, vendor show and event listings.

Neurodegeneration Drug Development

Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.

Cell Death and Disease

Hallmarks of Antibody Validation: Ranged Strategy

Hallmarks of Antibody Validation: Ranged Strategy. Binary models are not always readily available and can be time-consuming to produce for validating an antibody.

Hallmarks of Validation - Ranged Strategy

A ranged validation strategy includes both endogenous and heterologous models that express high, moderate, and low levels of the target of interest.

10 Must-have Markers for Senescence Research

Interested in studying senescence? Understanding cell cycle arrest is critical to many fields of research, including development, aging, and cancer.

Successful Immunofluorescence: Antibody Dilution & Incubation Conditions

Changing the primary antibody dilution and incubation conditions affects fluorescence intensity and signal-to-noise ratio in IF experiments.

Acquiring Cell Death

Mutations in cell death pathways, such as apoptosis, mitophagy, necroptosis, and autophagy, contribute to neuronal cell death and the progression of neurodegenerative diseases.

Overview of Cell Death

Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.

Solutions for Oncology Therapeutics Discovery

Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.

G-Protein-Coupled Receptors Signaling to MAPK/Erk

Explore the G protein coupled receptor signaling pathway and its diverse roles in cellular communication. Read more here.

Resources

The GFP Antibody #2555, GFP (4B10) Mouse mAb #2955, GFP (5G4) Mouse mAb #55494, and GFP (D5.1) XP® Rabbit mAb #2956 are not expected to detect Turbo GFP due to low sequence homology.

Cell Signaling Technology, Inc. Announces License in Personalized Cancer Diagnostics

Cell Signaling Technology, Inc. Announces License in Personalized Cancer Diagnostics.

Jak/Stat Utilization Table

The Jak/Stat Utilization Table displays the combinatorial use of tyrosine kinases and Stat proteins in cytokine/growth factor signaling.

Resources

We have performed CUT&Tag on dozens of cultured adherent and suspension cell lines, both human and mouse. So far, we have not noticed any significant difference in performance between these cell lines.

Resources

We always recommend starting with CST CUT&Tag-validated antibodies because CST scientists perform intensive in-house tests to exclude any antibody that tends to show non-specific tagmentation at open chromatin regions (a unique problem for the CUT&Tag assay). If a CUT&Tag-validated antibody is not available, we recommend starting with a CUT&RUN-validated antibody. Alternatively, you may want to try an antibody validated for ChIP, ChIP-seq, or an immunofluorescence assay. The antibody binding in CUT&Tag takes place in an intact nuclear environment, resembling conditions for antibody binding in immunofluorescence assays. If a non-CUT&Tag-validated antibody is used, it is imperative to confirm that there is no non-specific tagmentation, otherwise your results might not be reliable.

Overview of the Immune System

The immune system is composed of tissues, cells, and molecules whose primary function is to detect, respond to, and eliminate pathogens and transformed cells.

Resources

Our CUT&Tag kit is optimized for use with whole cells and works with a large number of cell lines, both adherent and suspension. While our kit works with pre-isolated nuclei because the Concanavalin A beads bind to glycoproteins on the surface of both cells and nuclei, we haven't found that pre-isolation of cell nuclei helps to increase the CUT&Tag signal. Pre-isolation of cell nuclei should only be necessary for cells that are not efficiently permeabilized by digitonin, such as yeast or plant cells, which both contain cell walls.

Resources

Yes. Cell fixation is recommended when the cells are very fragile (easily lysed during experiments), when the cellular signaling pathway is sensitive to the Concanavalin A beads used for cell binding, or when you hope to freeze the cell pellets for future use. If fixation is necessary, refer to the CUT&Tag Assay Kit Protocol, Appendix A to lightly fix cells prior to the CUT&Tag experiment. We find that live cells produce better results than fixed cells in the CUT&Tag assay. Therefore, we recommend using live cells, if possible, unless fixation is necessary for the scenarios mentioned above.

Resources

We recommend using our CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415 to make DNA libraries for CUT&Tag. Our DNA Library Prep Kit for Illumina (ChIP-seq, CUT&Tag) #56795, and its paired Multiplex Oligos for Illumina (Dual Index Primers) (ChIP-seq, CUT&Tag) #47538 or Multiplex Oligos for Illumina (Single Index Primers) (ChIP-seq, CUT&Tag) #29580 are not compatible with CUT&Tag DNA samples.

Cell Signaling Technology, Inc. wins Life Science Industry Awards in "Best Performing Antibodies" and "Best Breakthrough Products Cancer Research" Categories

Dec 2011, CST wins "Best Performing Antibodies" and "Best Breakthrough Products Cancer Research" Categories in Life Science Industry Awards.

Insulin Receptor Signaling

Discover the insulin signaling pathway and its role in glucose metabolism & diabetes. Learn more about insulin action here.

T Cell Receptor Signaling

Expert-reviewed interactive pathway providing a current overview of T Cell Receptor (TCR) Signaling.

CST Media Room

CST Media Room