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Protocol for PathScan Chemiluminescent Sandwich ELISA (for kits with Lyophilized Antibodies)
PathScan ELISA kits detect endogenous levels of key signaling proteins using a traditional sandwich-based assay format in phospho-specific or total protein options.
Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat
Application protocols: easy to follow directions describing the step by step experimental procedure.
PathScan Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.
Choosing the best ELISA kit for your research is important. Decide if the FastScan, PathScan, or PathScan RP ELISA kit is right for you.
ELISA Protocol for PathScan Stress and Apoptosis Signaling Antibody Array Kit: easy to follow directions describing the step by step experimental procedure.
In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.
The ELISA method has many variations, all of which rely on complex of an antigen and an antibody/enzyme conjugate. Signal is generated by turnover of substrate.
Testing ELISA kit lots for percent coefficient of variation (CV), signal/blank ratios, and optical density helps ensure scientific reproducibility.
PathScan Sandwich ELISA Protocol (for kits with Lyophilized Antibodies) from Cell Signaling Technology with recommended companion products.
PathScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
We typically recommend the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 that comes with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581 as it has been validated for use with the kit. The PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 was made specifically for this assay kit and for ELISA formats. We have not tested any other lysis buffers with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581, so we cannot guarantee the performance. The ingredients of the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 are as follows: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 ug/ml leupeptin.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Many types of ELISA have been developed to address different research needs from direct, indirect, to sandwich, competitive and multiplex. Learn here
The FastScan kits differ from the PathScan kits in that (1) they come with a positive control and (2) the assays can be performed much faster. With FastScan, the samples are incubated with a capture antibody conjugated with a proprietary tag and a detection antibody linked to HRP with no need for wash steps. The FastScan kits require the use of a specific lysis buffer, included in the kits, and are not compatible with other buffers. In terms of sensitivity, FastScan and PathScan are fairly equivalent.
Our PathScan® Total Androgen Receptor Sandwich ELISA Kit #12850 recognizes full-length AR protein and is predicted to also recognize the AR-V7 isoform.
Catalog numbers in tabular format for control cell extracts and proteins with links to product pages.
A table that lists shelf-life expectations for CST products (when stored under the recommended conditions).
PathScan Sandwich ELISA Antibody Pair Protocol: easy to follow directions describing the step by step experimental procedure.
Exactly what is ELISA? Learn about the enzyme-linked immunosorbent assay, the specific interaction between antibody & antigen. Click here.
FastScan ELISA kits detect key signaling proteins using a solution-based assay format and are validated to ensure reproducible results across lots and users.
Discover our ELISA guides and resources at CST, offering comprehensive protocols and guides for ELISA experimental design & optimization. Click here.
Discover the top epigenetic markers that you should look out for in prostate cancer research.
How does ELISA work? Understand ELISA principles for accurate molecule detection and quantification through antibody-based assays. Click here
Discover the key epigenetic drivers of breast cancer and the best products to target them.
Optimization is critical to obtaining reliable ELISA results. The design and development of an ELISA assay depends on the various components, including capture antibody, blocking buffer, diluent, sample concentration, and detection antibody
KinomeView Protocol: easy to follow directions describing the step by step experimental procedure.
