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A scientific resource for the IQ protein domain containing information on structure, function, and domain binding to calmodulin.
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
How does the choice of blocking protein—nonfat dry milk vs bovine serum albumen (BSA)—affect your western blot (WB) results? Watch the Tech Tips video.
The specificity of our 4E-BP1 (53H11) Rabbit mAb #9644 has been confirmed as follows:1) See “Western blot analysis of extracts from control HeLa cells (Lane 1) or HeLa cells with a targeted mutation in the gene encoding 4E-BP1 (Lane 2)” on the product webpage at https://www.cellsignal.com/products/primary-antibodies/4e-bp1-53h11-rabbit-mab/9644.2) 4E-BP1/2 DKO MEFs PMID: 28918902 (https://pubmed.ncbi.nlm.nih.gov/28918902/) Figure 6E
Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is meant for use, in tandem, with our pAG-MNase Enzyme #57813, which is a proprietary product.
We have performed retrieval comparisons with a number of antibodies and found, generally, that EDTA tends to result in stronger signals, both specific and potentially non-specific. If antibodies that are optimized with citrate retrieval are used with EDTA, further titration may be required in order to achieve an optimal signal. Conversely, use of citrate in some instances where EDTA is recommended could contribute to a weaker signal.
A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.
Rabbits only have one IgG isotype compared to four IgG isotypes in mice (IgG1, IgG2a, IgG2b and IgG3) and humans (IgG1, IgG2, IgG3 and IgG4). There are two types of rabbit light chains - κ and λ - with the κ light chain present as two different isotypes called K1 and K2. K1 is by far the most abundant isotype, representing between 70% and 90% of all light chains, whereas the remainder are usually a mixture of both K2 and λ-light chains. Therefore, most of our rabbit monoclonal antibodies have κ light chains.
Validated antibodies for high content screening from CST Cell Signaling Technology
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
A scientific resource for the F-Box protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.
SignalStar™ Multiplex IHC Kits & Reagents have not yet been validated for use in frozen tissues. We are currently in the process of validating our antibodies and protocols for use in fresh or frozen tissue.
Through in-house testing, we have found that the magnetic beads from our PTMScan HS kits aggregate and stick to the walls of some specific brands of tubes. Moreover, they cannot be washed off or spun off the sides of the tubes. This is an issue with specific brands of tubes themselves and not an inherent issue with the PTMScan bead product. Therefore, the following brands of polypropylene tubes are NOT recommended with magnetic bead PTMscan HS kits: 1. CellTreat #229441, polypropylene 1.5mL tube, non-sterile, non-pyrogenic2. Denville Scientific (Harvard Bioscience) #C3171, 1.5mL Posi-Lock tubes, polypropyleneThe following brand of microcentrifuge tubes is compatible with magnetic bead PTMscan HS kits:1. USA scientific tubes #1615-5500
Cell Lysis Buffer (10X) #9803 will interfere with the Bradford protein assay. This is because it contains detergent. To make the assay somewhat compatible, dilute your protein quantitation samples at least 10-fold with water or PBS to reduce the amount of detergent and consequentially lower the interference. This ten-fold dilution may still yield background absorbance. We recommend employing an alternative protein assay that is compatible with #9803, such as the BCA Protein Assay Kit #7780.
It remains difficult for scientists to predict the long-term health effects of COVID-19, particularly considering its diversity of effects during acute infection. The most severe impacts appear to manifest in tissues with high levels of vascularization (e.g., lungs, heart, kidney, liver), and it is evident that the virus elicits a significant inflammatory response in infected tissues
Yes, you may freeze your cells and pool them for a later CUT&RUN experiment. However, we would suggest a light fixation using 0.1% formaldehyde for 2 min before freezing the cells, in case the protein-DNA association inside the nuclei changes during the freeze-thaw of the cells.
A scientific resource for the CUE protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
We have several antibodies for detecting ribosomal protein S6 (rpS6) that are validated for IF-IC with mouse samples. These are S6 Ribosomal Protein (5G10) Rabbit mAb #2217 and S6 Ribosomal Protein (54D2) Mouse mAb #2317. Both of these antibodies are suitable for immunofluorescence in mouse cells.
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Molecular oxygen is an essential element for metazoan life. Among its many roles, it functions as the final electron acceptor (oxidizing agent) during oxidative phosphorylation, a metabolic chain-reaction that generates energy in the form of ATP. HIF-1 alpha
We recommend that you complete the BrdU cell proliferation assay as soon as you have the time. However, if you are unable to complete the experiment during the recommended time, you can complete the experiment through Step B: BrdU Incorporation and then pause. To do this, please follow the steps below:1. Add the fixing/denaturing solution and incubate for 30 minutes (Part C: BrdU Assay Step 1).2. Remove your solution and dry your plate at room temperature for 30 minutes. 3. Cover plates with parafilm and store at 4C.It is important to make sure your plate is completely dry before you cover with the parafilm and store it. Dry plates can be stored for up to 1 month at 4C, however, the signal of the assay may decrease slightly.
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
Identifying and quantifying myeloid subtypes is essential for understanding why different cell populations are activated in response to certain pathogens.
You may have seen a validation claim from your reagent supplier, but how can you be certain that your antibody is specific?
Our Ras (G12D Mutant Specific) (D8H7) Rabbit mAb #14429 and Ras (G12V Mutant Specific) (D2H12) Rabbit mAb #14412 will detect all three types of Ras (H-Ras, K-Ras, and N-Ras) if the respective mutations are present.
Therapeutic developments in treating Alzheimer’s, novel diagnostic tools, and new insights into neurodegeneration mechanisms were key topics at AD/PD 2023.
Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy and light chain.
Our GFP (D5.1) Rabbit mAb #2956 is expected to detect EGFP, eBFP2, and mCerulean3.
A scientific resource for the BEACH protein domain containing information on structure, function, and phospholipid binding.