3D video animation of histone modifications and their effects on chromatin activation
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
An interactive 3D molecular model depicting the cellular landscape of a nucleus and it's various protein subsets including DNA Damage, Replication, Transcription, etc.
A demonstration of the perils of deviating from Cell Signaling Technology's recommended protocols.
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
Comprehensive SimpleChIP® kits and ChIP validated antibodies from CST and validated ChIP reagents (primer pairs, Protein G agarose and magnetic beads
Detailed technical support information to support ChIP experimental success. LInk to CST's comprehensive ChIP protocol.
Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.
Chromatin IP (ChIP) Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
A scientific resource for the FF protein domain containing information on structure, function, and binding to phospho-serine motifs.
Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Summary of human oncogenes and tumor suppressor genes sorted by signaling pathway.
Immunohistochemistry Protocol (Frozen): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.
Pre-Clinical IHC Tools for Mouse Models
A kinome diagram showing the CK1 family of protein kinases.
A kinome diagram showing the human tyrosine kinase group. This group of protein kinases comprises receptor and non-receptor tyrosine kinases.
Searchable Human Kinome showing the AGC, Tyrosine Kinases, TKL, CAMK, STE, CMGC, CK1 and atypical kinase groups.
Application protocols: easy to follow directions describing the step by step experimental procedure.
A scientific resource for the PWWP protein domain containing information on structure, function, and domain binding.
Assay Kits from CST contain ChIP validated antibodies and control PCR primers to assay for pluripotency markers or epigenetic mark status in human or mouse samples.
CST provides multiple options for avoiding the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions that can damage both antibody epitopes and the integrity of the chromatin.