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In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.
In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.
We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
ELISA Protocol for PathScan Stress and Apoptosis Signaling Antibody Array Kit: easy to follow directions describing the step by step experimental procedure.
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spectra of DRAQ5 and DyLight 680 overlap, and the signal produced by each dye cannot be distinguished.
KinomeView Protocol: easy to follow directions describing the step by step experimental procedure.
PEG (polyethylene glycol) has been added to our Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5151 and Anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate) #5257 to increase their solubility and stability for various applications, including cellular and in vivo imaging. Internal Fluorescent Western and In-cell Western testing shows that the addition of PEG to these secondaries not only significantly increases signal but also decreases background fluorescence.
Neuroinflammation is the activation of an immune response in the CNS by the microglia and astrocytes. While not linked mechanistically to neurodegenerative diseases, neuroinflammation is associated with the progression of Alzheimer’s disease, Parkinson’s
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
Learn how RNA-binding proteins influence RNA regulation and epitranscriptomics and contribute to the progression of a variety of diseases.
Knockout cell lines and modulating the target's phosphorylation state are experimental controls that can be used to confirm specificity of your cell type.
Chronic neuroinflammation is associated with neurodegenerative diseases like Alzheimer’s, Parkinson’s, amyotrophic lateral sclerosis, and others.
Mutations in cell death pathways, such as apoptosis, mitophagy, necroptosis, and autophagy, contribute to neuronal cell death and the progression of neurodegenerative diseases.
Epigenetic regulation, including aberrant DNA methylation and histone modifications, have been linked to Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, multiple sclerosis, and amyotrophic lateral sclerosis.
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
Neuroinflammation is a condition observed in the central nervous system (CNS) in response to infection, toxic metabolites, traumatic injury, or autoimmunity.
Validation for IF antibodies at CST Cell Signaling Technology
The immune system is composed of tissues, cells, and molecules whose primary function is to detect, respond to, and eliminate pathogens and transformed cells.
A binary approach is one of the best ways to evaluate antibody specificity.
Stress granules are a cellular defense mechanism against stress but disruption in disassembly can lead to toxic proteins and neurodegenerative diseases.
Modulation of CNS function occurs through synaptic neurotransmission, which is the signaling from the axon terminal of one neuron to the dendrites of another via molecules called neurotransmitters.
The key to identifying neurons, microglia, oligodendrocytes, and astrocytes lies in using antibodies that target protein biomarkers specifically expressed and localized within these cells.
Trademark information appearing on Cell Signaling Technology website.
Beyond our recommended immunofluorescence protocols, here are additional considerations when planning your IF imaging experiment.
Streamline your neurodegeneration therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Authors of an article in Science Signaling investigated the effects of a Myc tag insertion location on antibody function. Their efforts focused on the most cited antibody for Myc, monoclonal antibody 9E10.
Hallmarks of Antibody Validation: Ranged Strategy. Binary models are not always readily available and can be time-consuming to produce for validating an antibody.
Cellular senescence is stable cell cycle arrest linked to aging and cancer and other disease states, including those associated with inflammation. It is induced by DNA damage, oncogenic signaling, and telomere shortening.
A binary approach is one of the best ways to evaluate antibody specificity. By testing an antibody in biologically relevant positive and negative expression systems.
