Flow Cytometry is readily amenable to intracellular target detection and can be successfully applied to the study of complex signaling pathways
The TGF-β Antibody #3711 detects recombinant TGF-β1, TGF-β2, and TGF-β3. The antibody also detects endogenous levels of the TGF-β1 precursor proteins.
Both our AIF Antibody #4642 and our AIF (D39D2) XP Rabbit mAb #5318 are predicted to detect full-length AIF [amino acids 1-613 (predicted to be 67 kDa)], mitochondrial AIF [minus amino acids 1-54 (predicted to be 62kDa) and anchored to the inner mitochondrial membrane], and soluble/mature AIF [minus amino acids 1-101 (predicted to be 57kDa) and released to the cytoplasm to be translocated to the nucleus]. Although the apparent molecular weight is reported as 67kDa, the dominant band shown in our western blot images most likely represents mitochondrial AIF (predicted to be 62 kDa).
An antibody shouldn’t be one of the variables in your experiment. Find out why customers rank CST highest for antibody specificity and sensitivity.
Multiplex IHC - Being able to visualize multiple targets simultaneously is important in research fields like immunology & oncology. Learn more here.
For antigen retrieval in a pressure cooker, bring slides submerged in buffer up to 125C, maintain for 30 seconds, then cool to 90C and maintain that for 10 sec. Remove the container from the device and cool it on the bench top for 10 minutes prior to washing.
We have several monoclonal antibodies for detecting total Akt protein that are validated for IHC-P with mouse samples. These are as follows: Akt (pan) (C67E7) Rabbit mAb #4691Akt (pan) (11E7) Rabbit mAb #4685Akt (pan) (40D4) Mouse mAb #2920 All of these work well and there is no preference for this application.
Necrosis has been classically defined as an unprogrammed form of cell death that occurs in response to overwhelming chemical or physical insult.
A Hallmark of Cancer, sustaining proliferative signaling is used by cancer cells to stimulate growth and relies on Akt, MAPK/Erk, and MTOR pathways.
Lymphoid lineage cells can be characterized using antibodies for cell type-specific markers, allowing cells to be visualized using flow cytometry or IHC.
A scientific resource for the 14-3-3 protein domain containing information on structure, function, and binding to phospho-serine and phospho-threonine motifs.
MEDx and CST announce expansion of their ongoing partnership for companion diagnostic (CDx) development using CST® antibodies to meet growing demand in China.
U.S. Patent No. 6,441,140, directed to the production of a novel class of motif-specific, context-independent antibodies, highly useful for the identification of subclasses of protein kinase substrates.
Feb 2008, CST continued collaboration with AstraZeneca to provide CST’s PhosphoScan® Platform for kinase inhibitor proteomic profiling.
Cell Signaling Technology, a leading provider of antibodies, kits and services, announced today that it has won two Life Science Industry Awards® for "Best Antibodies" and "Most Useful Website."
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
CST and The Michael J. Fox Foundation (MJFF) announce a partnership to develop high-quality monoclonal antibodies against promising Parkinson’s disease (PD) targets.
What is glycolysis? Glycolysis and oxidative respiration are the two metabolic pathways used to convert biochemical energy into ATP to fuel cell function.
Announce partnership with this local 1% for the Planet startup to help share and promote best practices for sustainability. Also, to encourage other companies to do the same by promoting recent sponsorship of NSTC as part of our ESR activities.
Here is a list of the most commonly asked questions with detailed answers to support researchers using InTraSeq™ Single Cell Analysis.
