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Antibody 5G10, which targets S6 ribosomal proteins, can be used as a control for formaldehyde fixation conditions and sample quality in IF experiments.
Our BSA #9998 is tested for protease activity and each lot can contain up to 0.005 units/mg. However it is not tested for globulins, DNase, RNase or endonuclease activity. Therefore, we are unable to say whether or not the product actually contains them or at what percentages.
New antibodies for Alzheimer's research were presented at SfN 2022, including tools to study neuroinflammation in glial cells like microglia and astrocytes
Depending on the target, the FastScan™ ELISA Cell Extraction Enhancer Solution #25243 usually increases the sensitivity/signal and performance of the kit. Therefore, if it is omitted, your signal may be lower, however the extent of this effect will vary from kit to kit. We recommend using the enhancer to avoid this possibility. If you forgot to add the enhancer to the FastScan™ ELISA Cell Extraction Buffer #69905 that was used to make the sample lysate, you can spike in the 50x Enhancer solution afterwards (and always add the enhancer to the extraction buffer that is used to dilute the samples). We believe the enhancer increases the signal by helping increase the recognition and binding of the target protein by the antibodies in the kit.
Antibodies, and proteins in general, are less susceptible to degradation when stored at higher concentrations (i.e., greater than or equal to 1.0 mg/ml). Therefore, because our off-the-shelf antibodies are offered over a wide range of concentrations, BSA is included in the storage buffer as a stabilizer.
A scientific resource for the HECT protein domain containing information on structure, function, and domain binding in ubiquitin-mediated protein degradation.
A scientific resource for the GAT protein domain containing information on structure, function, and domain binding to ubiquitin and GTP-bound ARF.
Much has been written about the hallmarks of cancer, but we expand on the story with The Guide to the Hallmarks of Cancer Research Targets.
Our Cell Fractionation Kit #9038 provides a fast and efficient way of separating cultured cells into three distinct fractions for analysis by SDS-PAGE and western blotting. We have not tested fractionated samples using mass spectrometry in-house and it is difficult to speculate how well they would work. The buffer contains SDS so you may need to use a commercially available SDS purification kit if you would like to prepare the fractionated samples for mass spectrometry by in-solution digestion; in-gel digestion would account for SDS removal in the extraction already. We cannot guarantee activity by mass spectrometry as it is not the primary intended use of this kit.
A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which leads to the release of bound PTM peptides, denatures the antibodies. This denaturing step alters the conformation of the antibodies. Therefore, employing these antibodies for subsequent enrichments may produce inconsistent results in PTM peptide enrichment.
Due to the fact that the total α-Adducin (D7T7R) Rabbit mAb #70174 does not recognize this band in our in-house testing, the 150kDa background band seen with our Phospho-α-Adducin (Ser12) (E5X8Y) Rabbit mAb #84214 is likely an unrelated protein induced by nocodazole.
A scientific resource for the DH protein domain containing information on structure, function, and domain binding during Rho GTPase activation.
This webinar will highlight how antibody specificity affects reproducibility in biomedical research and will introduce viewers to the key requirements for proper antibody validation.
Sometimes understanding complex signaling is overwhelming. Try thinking about it as the wiring on a construction site. Maybe that will help!
A scientific resource for the EVH1 protein domain containing information on structure, function, and binding to a proline-rich motif to regulate actin dynamics.
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
A multiple antibody strategy is a powerful approach to antibody validation. This provides confidence that antibodies are binding the correct biomolecule.
When performing In-Cell Western assays with DyLight 680-conjugated secondary antibodies, we recommend using either Hoechst 33342 or DAPI as a nuclear dye. DRAQ5 cannot be used because the emission spectra of DRAQ5 and DyLight 680 overlap, and the signal produced by each dye cannot be distinguished.
Did you know that an estimated 18-36% of lab cell lines are believed to be misidentified or cross-contaminated with another cell line?
Since the buffers in the Cell Fractionation Kit #9038 contain SDS, you should use a commercially available ready-to-use, detergent- and reducing agent-compatible assay to quickly measure total protein concentration (A660nm) compared to a protein standard.
In-house, we tested AMPK Control Cell Extracts #9158 and mouse liver extract, blotting with AMPKα2 Antibody #2757 (approved for human and monkey) and a competitor AMPKα2 antibody (approved for mouse reactivity). We observed no signal with AMPKα2 Antibody #2757 in the mouse extracts, confirming AMPKα2 Antibody #2757 is not approved for mouse reactivity. Blotting with the competitor antibody we observed very weak signal in AMPK Control Cell Extracts #9158 and strong signal in mouse liver extract; therefore, suggesting AMPK Control Cell Extracts #9158 may not be an appropriate control for measuring AMPKα2 levels.
There are pros and cons to using monoclonal antibodies instead of polyclonals, and the same is true for using antibodies from rabbit hosts over mice hosts.
Unfortunately, separation of nuclear and cytoskeletal proteins is not possible using our Cell Fractionation Kit #9038. Because cytoskeletal proteins are involved in the stabilization and localization of the nucleus, the resulting nuclear fraction will also include these proteins.
CST Science Scholarships consists $10k scholarships, awarded to high school juniors, from Massachusetts, to pursue a degree in a scientific discipline.
As a company focused on cancer research, we planned a volunteering event with the American Cancer Society Hope Lodge.
Apoptosis is a regulated form of programmed cell death (PCD) that occurs without external influence.
Interactive 3D molecular model depicting key adhesion signaling pathway nodes within their cellular landscape and integrated with PhosphoSitePlus protein pages.
CST PTMScan tutorial video explains how to use antibodies to quickly and efficiently enrich PTM peptides for mass spectrometry-based proteomics