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A scientific resource for the VHL protein domain containing information on structure, function, and domain binding to hydroxylated proline residues.
At CST, we recommend fluorochrome-conjugated F(ab')2 fragment secondaries as they reduce the non-specific background that is usually associated with their whole molecule counterparts. Whole molecule secondaries have the highest capacity for dye conjugation and, thus, are likely to yield the brightest signal. Their major downside is background, as many cell types express Fc receptors that these are likely to stick to without sufficient blocking.F(ab')2 have had their Fc regions removed through pepsin digestion, leaving two antigen binding domains.F(ab')2 antibodies have reduced capacity for dye conjugation which may result in a slightly weaker signal when compared to their whole molecule counterpart. Despite this, we find this format to provide an optimal balance between signal and noise.Monovalent F(ab) fragments are highly penetrant due to their small size, but also yield extremely low intensity. In our experience, these are best utilized as blocking reagents in their unconjugated …
A Hallmark of Cancer, sustaining proliferative signaling is used by cancer cells to stimulate growth and relies on Akt, MAPK/Erk, and MTOR pathways.
Any form of cell culture contamination can ruin your day and destroy your hard work, but mycoplasma contamination is particularly devastating.
CST Technical Support Article
We have tested both the S6 Ribosomal Protein (54D2) Mouse mAb #2317 and the S6 Ribosomal Protein (5G10) Rabbit mAb #2217 for IP; however, both failed to meet our standards for IP recommendation. While each antibody did appear to pull down at 1:50, the signal was equivalent in the IgG control lane therefore we do not recommend using either product for IP.
A scientific resource for the RGS protein domain containing information on structure, function, and domain binding during G protein-mediated signaling.
A scientific resource for the UIM protein domain containing information on structure, function, and domain binding to ubiquitin.
The innate immune response provides a quick response to pathogens by many mechanisms, including cytokine production and complement activation.
A scientific resource for the MIU protein domain containing information on structure, function, and domain binding to ubiquitin during vesicle trafficking.
Antibody 5G10, which targets S6 ribosomal proteins, can be used as a control for formaldehyde fixation conditions and sample quality in IF experiments.
Senescent cells increase in number during aging and have been implicated in the decline of organismal function over time, as well as in the progression of age-related diseases.
A scientific resource for the ENTH protein domain containing information on structure, function, and domain binding to phospholipids during endocytosis.
A scientific resource for the PB1 protein domain containing information on structure, function, and domain binding in cytoskeletal signaling proteins.
A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.
A scientific resource for the DH protein domain containing information on structure, function, and domain binding during Rho GTPase activation.
The antibody in our Active Rho Detection Kit #8820 detects RhoA, RhoB, and RhoC. Regrettably, there is no way to distinguish between the three proteins.
A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.
What is glycolysis? Glycolysis and oxidative respiration are the two metabolic pathways used to convert biochemical energy into ATP to fuel cell function.
A scientific resource for the EH protein domain containing information on structure, function, and domain binding during endocytosis and vesicle trafficking.
We understand some customers choose to pursue TMT labeling for PTMScan proteomics analysis, but we do not have a defined protocol for this method. However, there are two questions we can address:
Tumor cells adopt novel mechanisms to acquire nutrients and reprogram metabolic pathways to meet their increased bioenergetics demands. CST provides tools to detect and measure key metabolic changes.
As a company focused on cancer research, we planned a volunteering event with the American Cancer Society Hope Lodge.