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While we do not specifically test our antibodies using co-IP, we expect IP-approved antibodies to work well for co-IP experiments. As each protein-protein interaction is unique, optimization of lysis conditions and the inclusion of proper controls are necessary for a successful experiment. More information on experimental design for IP and co-IP experiments can be found here.
For co-IPs, we suggest blotting for the target protein first to confirm pull-down. Once confirmed, blotting for additional proteins in the complex can be performed. If your protein of interest runs at a size similar to the heavy or light chain of IgG, we recommend the use of a conformation-specific or
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Due to the expected and typically low yield of CUT&Tag DNA, we recommend using all 30 µL of the CUT&Tag DNA sample (from Section VI of the CUT&Tag protocol #77552) for library amplification. While CUT&Tag DNA libraries generated for histone modifications typically show some signal on the Agilent Bioanalyzer or TapeStation systems, libraries generated for non-histone proteins such as transcription factors and cofactors often have very weak or even no visible signal on Bioanalyzer or TapeStation systems, but they still generate NGS results with high-mapping rates, high numbers of identified binding peaks, and decent signal-to-noise ratios across the whole genome. For CUT&Tag libraries where the Bioanalyzer or TapeStation system is unable to identify the average size of the library, we suggest using a size o…