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Study the intricate machinery of the apoptosis signaling pathway. Click here to learn more and gain insights into programmed cell death mechanisms.
Culturing cells in the lab? Following these tips will add to your success and help you avoid wasted time or the dreaded cell contamination!
We recommend using the lysis buffer provided with your ELISA kit. Our ELISA kits include one of two lysis buffers, either Cell Lysis Buffer 10X) (#9803 or PathScan Sandwich ELISA Lysis Buffer (1X) #7018. All of our ELISA kits are optimized for use with samples generated in the recommended lysis buffer.
Do polyclonal have an advantage over monoclonals for chromatin immunoprecipitation? We examine the assumption and implications for ChIP and ChIP-seq.
Yes, our DYKDDDDK Tag antibodies will detect 3X FLAG tag as long as the FLAG tag sequence is located on the N-terminal or C-terminal end.
Discover the PI3K Akt pathway and its crucial role in cell growth and survival. Click here to learn more & gain insights into this important signaling cascade
Cancer cells stimulate the growth of blood vessels to supply nutrients to tumors. Angiogenesis is the formation of new blood vessels from pre-existing blood vessels. This plays an important role in tumor growth.
Our products can be expected to perform at optimal levels up until the printed "Best" by date on the vial when stored under the recommended conditions. Many of our products will work well after the best by date has passed, however we cannot guarantee their optimal performance after this date. We routinely quality control test our antibodies. Our best by dates are based on the date of packaging and may be extended for the same lot, based on the product passing recent QC testing.
The composition of our DNA Elution Buffer #10009 is 10 mM Tris-Cl, pH 8.5.
Both enzymatic digestion and sonication work when performing ChIP for histones, transcription factors, and cofactors. However, we have found that enzymatic ChIP gives higher levels of enrichment than sonication ChIP for transcription factors and cofactors, especially those with low expression. We have also found that some transcription factors and cofactors only work in enzymatic ChIP and not sonication ChIP. That being said, sonication ChIP kit has its advantage when dealing with fibrous or fatty tissue types. Please refer to our online video (https://www.cellsignal.com/learn-and-support/videos-and-webinars/webinar-enzymatic-or-sonication-protocol) for which kit to choose for your ChIP experiment.
Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is meant for use, in tandem, with our pAG-MNase Enzyme #57813, which is a proprietary product.
Expert-reviewed interactive pathway providing a current overview of the molecular and cellular biology of Alzheimer’s Disease.
Explore the intricate Wnt Beta Catenin pathway & its role in cell development and disease. Learn more about the signaling cascade of this crucial pathway.
We're upping the ante on conservation. Cell Signaling Technology does Earth Month now. Because really, there's too much Earth for one week to hold.
Cancer cells invade local tissue and spread to distant sites via two distinct, but similar processes known as invasion and metastasis.
A scientific resource for the SWIRM protein domain containing information on structure, function, and binding to either DNA or acetylated lysine residues during chromatin remodeling.
A scientific resource for the START protein domain containing information on structure, function, and domain binding.
Validated antibodies for high content screening from CST Cell Signaling Technology
Pre-Clinical IHC Tools for Mouse Models
Although we are unable to guarantee that both our Protein A Agarose Beads #9863 and Protein G Agarose Beads #37478 will work after being stored at -20C, we have used beads that have been frozen and they have worked well. After thawing the beads, ensure they fully resuspended in the buffer with no aggregation and store the beads at 4C after use.
The extra band(s) around 24 kDa using our Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 are most likely mono-ubiquitylated H2AX. H2AX, like H2A, is mono-ubiquitylated.
A scientific resource for the TUDOR protein domain containing information on structure, function, and domain binding to methylated-lysine residues in chromatin remodeling.
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
No matter your research area, we’ve all needed to answer this deceptively easy question: how much of a particular protein is present in my samples?
Specific Aims set the stage for your grant proposal and are often considered the most important part, so make sure you're concisely conveying your goals.
Complementary strategies provide vital information regarding antibody specificity or functionality and can be tailored to the nature of the downstream assay.
The specificity of our Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 has been confirmed as follows:1) see the western image on the product webpage at https://www.cellsignal.com/products/primary-antibodies/phospho-ampka-thr172-40h9-rabbit-mab/2535. The specificity of #2535 has been confirmed on a binary model (i.e., on extracts from C2C12 cells, untreated or oligomycin-treated (0.5 µM). 2) shRNAPMID: 28553939 (https://pubmed.ncbi.nlm.nih.gov/28553939/) Figure 1b3) CRISPR/Cas9 PMID: 30415923 (https://pubmed.ncbi.nlm.nih.gov/30415923/) Figure 3A
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
We recommend dilution of the primary antibody into PBS containing 1% BSA and 0.3% Triton X-100. BSA acts as a carrier protein and improves antibody stability in dilute environments while Triton X-100 aids in ensuring maximum cell coverage. We recommend overnight incubation to allow maximum time for antibody binding. If the incubation time needs to be shortened, we generally recommend at least 2 hours at 37C but performance cannot be guaranteed.
For PTMScan® enrichments, we recommend a liquid chromatography (LC) gradient length of 90 minutes and replicate injections for each enrichment. Therefore, four PTMScan enrichments will have a total of eight liquid chromatography-mass spectrometry (LC–MS)/MS runs. For the immobilized metal affinity chromatography (IMAC) based phosphopeptide enrichments, the sample complexity requires a longer gradient length of 120 minutes, also performed with replicate injections for each sample.