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It is possible to see a doublet at ~100 kDa for Grp94 due to post-translational modifications. Here is a link to our PhosphoSitePlus database detailing the possible post-translational modifications for Grp94: https://www.phosphosite.org/proteinAction.action?id=6928&showAllSites=true.
CST now offers cost-effective, efficient, and convenient reagent kits to help facilitate western blot, immunofluorescent, and immunohistochemistry experiments.
The California Supply Chains Act requires CST to disclose its current practices to combat slavery and human trafficking in five specific areas.
From our limited in-house testing, we would suggest leaving glycerol on the plates for 3-5 days at most. We have not tested longer time periods than this.
This interactive guide shows the key cell and functional state markers for tumor-infiltrating immune cells in humans.Web Location: Immunology and Inflammation
The calculated molar extinction coefficient of rabbit IgG at 280 nm is approximately 210,000 M-1cm-1.
It is critical that our Phospho-p53 (Ser15) Antibody #9284 be diluted into 5% w/v non-fat dry milk, 1X TBS, 0.1% Tween® 20 and incubated at 4°C with gentle shaking, overnight. Diluting the antibody in BSA will result in high background on the blot.
The D8L9T CD36 clone from CST (# 14347) exhibits superior sensitivity and specificity by IHC and Western blot when compared to a CD36 clone from an alternative vendor.
Protein A predominantly binds intact IgG and does not bind denatured IgG well. It can be used in IP experiments to help avoid the potential masking of signal by denatured IgG heavy and light chain.
How can intracellular and extracellular protein readouts be combined in flow cytometry? This video offers protocol advice from our flow expert.
The concentration of the pAG-MNase Enzyme #57813 in our CUT&RUN kits is 25ug/ml.
Unfortunately, our PD-1 or PD-L1 antibodies have not been tested in neutralization assays. Therefore, we cannot guarantee their performance.
The Hematoxylin #14166 formula is based on Gill's formulation however, this formulation does not contain alcohol or mercury.
Our COX IV (3E11) Rabbit mAb #4850 will specifically label human cells and not mouse, and our COX IV (D6I4K) Rabbit mAb (Rodent Specific) #38563 will specifically label mouse cells and not human.
Both enzymatic digestion and sonication work when performing ChIP for histones, transcription factors, and cofactors. However, we have found that enzymatic ChIP gives higher levels of enrichment than sonication ChIP for transcription factors and cofactors, especially those with low expression. We have also found that some transcription factors and cofactors only work in enzymatic ChIP and not sonication ChIP. That being said, sonication ChIP kit has its advantage when dealing with fibrous or fatty tissue types. Please refer to our online video (https://www.cellsignal.com/learn-and-support/videos-and-webinars/webinar-enzymatic-or-sonication-protocol) for which kit to choose for your ChIP experiment.
Landing page provides links to CST promos and CST events.
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3.
Content Overview for SARS-COVID Research
We currently have four different secondary antibodies approved for use in fluorescent western blotting, depending on species and wavelength of detection:
Learn more about commonly used chemical modulators to study cellular physiology and biology.
Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubulin isoforms A, B, and C. The antigen sequences for these antibodies have 96% homology with beta 2C, beta 4B, beta 5B, and beta 4A isoforms 1, 2, and 3, and 95% homology with beta 2C isoforms A and B. Therefore they are likely to detect these subtypes.
Technical Support Confirmation Request - Cell Signaling Technology
Yes, you can use BSA #9998 as a carrier for cytokines. It should be dissolved into 1X PBS and sterile filtered.
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Why are antigen retrieval and antibody diluent important for the immunohistochemistry protocol? Our IHC expert offers protocol advice.
We have only tested our Senescence β-Galactosidase Staining Kit #9860 with adherent cells. However, we commonly recommend immobilizing suspension cells to a glass slide by using a cytospin. Once the cells are mounted to the slide, you can follow our recommended protocol. Our customers have had success using this method.
该视频重点介绍肿瘤免疫微环境相关的主要参与细胞、调节分子和信号通路,并为各位听众提供在上述领域的主要评价指标和新的研究方法作为参考。
该视频将重点介绍与细胞骨架、细胞基质动态调控及EMT相关的主要调节分子和信号通路,并为各位听众提供在上述领域的主要评价指标作为参考。
Our Anti-mouse IgG, HRP-linked Antibody #7076 shows approximately 25% reactivity to mouse IgM.
Our Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 detects over-expressed levels of PKCalpha and beta. It also detects endogenous levels of PKC in Jurkat + CalA extract and possibly weakly in other endogenous models tested. Our Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106 and Phospho-Akt (Thr308) Antibody #9275 detect over-expressed levels of PKCalpha and beta, but do not detect endogenous PKC. Our Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 detects over-expressed levels of PKCalpha and beta and may also weakly detect endogenous PKC in Jurkat + CalA extract, but not in other endogenous models tested. Our Phospho-Akt (Thr308) (D25E6) XP Rabbit mAb #13038 does not detect over-expressed PKC and is the best choice of the Phospho-Akt (Thr308) antibodies if this is a concern.