Basket Updated
0
Items added
We use the Octet® RED96.
Drs. Benjamin Wolozin and Dorothee Dormann present recent research on phase separation mechanisms regulating stress granule proteins implicated in ALS, frontotemporal dementia, and other neurodegenerative diseases.
To enable surface characterization of chimeric antigen receptors (CARs), scientists at CST developed versatile and specific antibodies that detect the linker sequence of scFv domains. Watch the poster presentation from AACR.
Our Insulin (C27C9) Rabbit mAb #3014 was produced with an antigenic peptide corresponding to residues within the insulin B chain of the human insulin protein (UniProt ID #P01308; https://www.uniprot.org/uniprot/P01308). Therefore, it is expected to recognize both proinsulin and mature insulin. While this antibody is not approved for use in western blotting, it should be able to detect the proinsulin protein if the gel is run carefully enough.We also offer an Insulin (L6B10) Mouse mAb #8138, which is approved for use in western blotting on rat samples. This antibody detects both proinsulin and insulin as well.
It is not uncommon to see particulates in the protein substrate tubes provided with the GTPase kits. After extensive testing, we have found that these are not a cause for concern. We recommend briefly centrifuging the product before use to clarify the material.
CST has not tested this kit successfully on tissue sections (frozen or paraffin-embedded), so we do not have a tissue-specific protocol to provide. At one time we tried using this kit on paraffin-embedded (FFPE) tissue slides, but unfortunately, we did not obtain good results. We have, however, spoken with customers who use this kit on frozen tissue and there are several publications that have successfully used this kit under these conditions. We have listed a few of them below for reference.B. G. Childs, T. J. Bussian, D. J. Baker (2019) Cellular Identification and Quantification of Senescence-Associated Beta-Galactosidase Activity in Vivo Methods Mol Biol. 1896: 31-38.Baker, D. J. et al. (2004) BubR1 insufficiency causes early onset of aging-associated phenotypes and infertility in mice. Nat. Genetics 36, 744-749.Swarbrick, A. et al. (2008) Id1 cooperates with oncogenic Ras to induce metastatic mammary carcinoma by subversion of the cellular senescence response. Proc Natl Acad Sci U S A…
Discover the TGF Beta signaling pathway and impact on cell growth & tissue homeostasis. Learn here the mechanisms behind this vital signaling cascade.
Our Akt Antibody #9272 detects all 3 isoforms of Akt: Ak1, Akt2, and Akt3.
A comprehensive, step-by-step video of the CUT&RUN Protocol, from sample preparation to CUT&RUN DNA purification and qPCR quantification. Expert tips for critical steps and experimental design are highlighted to help you successfully complete the assay.
We do not perform enzymatic activity assays internally using the Cell Lysis Buffer (10X) #9803, so we are unable to guarantee that the enzymatic activity will be conserved. We routinely use this buffer in immunoassays such as western immunoblot, IP, and ELISA without issue.As a reference, the components of the Cell Lysis Buffer (10X) #9803 are provided below:20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin
Learn DNA library preparation for ChIP-seq and CUT&RUN using the Illumina® protocol, from end repair & adaptor ligation to PCR enrichment & cleanup.
Multimers are likely to demonstrate higher apparent affinity due to avidity effects. However, an exact recommendation for a competition assay would depend on other assay parameters, specifically assay format (in-tandem, classical sandwich, or premix), tags present on other proteins being used, and detection method.If you want to immobilize ACE2, we recommend using the Human ACE2 (18-652) Recombinant Protein (mFc-Tag) #83986 due to the ease of immobilization by protein A or anti-Fc. If you want ACE2 in solution, we recommend using the Human ACE2 (multimeric) (18-652) Recombinant Protein #85054 if you are immobilizing other proteins by protein A or anti-Fc. This is because the multimeric protein does not cross-react at protein A or anti-Fc surfaces. If you would like ACE2 in solution but are not using protein A or anti-Fc surfaces for immobilization, either protein should work well. Finally, if you want the most potential avidity in solution, then the multimeric protein is preferable.
Expert-reviewed interactive pathway providing a current overview of the molecular and cellular biology of Alzheimer’s Disease.
It should not matter whether you spike in IgG2a or IgG1 antibodies when using the SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit #69486, as the kit is measuring the ability of the sample to block the ACE2-RBD interaction and is not directly measuring the antibodies. Specifically, this kit is designed to detect blocking antibodies present in human serum or plasma samples.
There is 50 µl of each antibody component that is listed in the StemLight Pluripotency Antibody Kit #9656. The kit components are pre-optimized for parallel use in immunofluorescent analysis. There are enough reagents for 100 assays based on a working volume of 100 µl. Another way to think of it is that if you need 100 µl of diluted antibody solution per sample/test, you will be using 0.5 µl of antibody (diluted into 100 µl) when used at the recommended 1:200 dilution.
Unfortunately, we have not internally validated the use of acidic samples with our Cyclic AMP XP(R) Assay Kit #4339. We have had customers try this method, preparing their samples with 0.1M HCL and then neutralizing. The customers did not have success with this method.If using acidic sample preparation, we recommend further diluting samples in 1X PBS after neutralization, prior to adding samples to the plate. Again, as we have not internally validated this, we cannot guarantee this kit will work with acidic samples.
Expert-reviewed diagram providing a current overview of the Senescence Signaling pathway with references to its role in cell cycle
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
Multiplex Oligos for Illumina Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.
Multiplex Oligos for Illumina Protocol (Single Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.a
This sonication step is used simply to destroy the cellular and nuclear membranes, as the digested chromatin is still inside the permeabilized nucleus and needs to be released into the supernatant. This sonication step is done in 1xChIP Buffer which is not an optimal sonication buffer and will not further fragment the chromatin.
When using the SimpleChIP® and SimpleChIP® Plus Enzymatic Chromatin IP Kits (#9002, #9003, #9004 and #9005), the Buffer A solution will permeabilize the cells using SDS. Once the cells are porous, they are transferred to a favorable environment for micrococcal nuclease (Buffer B) to allow for the digestion of chromatin.