Basket Updated
0
Items added
CST Technical Support Article
The typical DNA yields from ChIP experiments range from 0.2-2 ng/µl when targeting transcription factors or cofactors and from 2-20 ng/µl when targeting histone modifications. If the concentration is within this range, then you should be able to continue with ChIP-seq. Our DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795 can generate a library with 0.5ng ChIP-DNA.
An orthogonal strategy for antibody validation involves cross-referencing antibody-based results with data obtained using non-antibody-based methods.
For antigenic targets where expression of the protein is very low or unknown, the use of recombinant proteins or exogenous expression in a surrogate cell line may be necessary for antibody validation.
Studies of neurodegeneration using mouse models of Alzheimer's disease can now employ whole-brain tissue clearing techniques to better visualize expression of β-Amyloid and other hallmarks of disease.
Hallmarks of Antibody Validation: Ranged Strategy. Binary models are not always readily available and can be time-consuming to produce for validating an antibody.
Identify and quantify membrane proteins in a total proteome study for a more complete view of proteins on the cell surface.
This video demonstrates the technique used for splitting adherent cell lines with trypsinization.
Products and Related Resources for Type I Interferon and Viral Restriction Factors SARS-CoV-2 Research
Quantitative analysis of the SMAD signaling pathway using ELISA, high content imaging, and high-resolution confocal approaches were used to study the regulation of angiogenesis and cell proliferation.
This video describes the application of robust multiplex imaging technologies to interrogate myeloid and lymphoid lineages to provide spatial insights of many cell types in the tumor microenvironment.
Watch this webinar to learn how SignalStar Multiplex IHC can speed your spatial biology discoveries up to 70% with optimized, ready-to-go panels.
Learn how CUT&Tag cuts down your chromatin profiling experiment time. You’ll get the same great data with a fraction of the DNA library prep time and improved signal-to-noise.
A multiple-antibody strategy is a powerful approach to antibody validation. The most common method to achieve this is to immunoprecipitate (IP) the target with one antibody and subsequently detect it by western blotting with another antibody against the
A troubleshooting guide for CUT&Tag–a ChIP-seq alternative with lower sample requirements, lower sequencing cost, and improved signal-to-noise for profiling chromatin and protein-DNA interactions.
Hallmarks of Antibody Validation-Complementary Strategies that Employ Western Blot, Immunohistochemistry (IHC), Immunofluorescence (IF/ICC), Flow Cytometry, Histone Antibody, ChIP-qPCR, ChIP-seq.