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Our Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb #3024 recognizes Tyr1189/1190 in isoform 1 (Long) and Tyr1177/1178 in isoform 2 (Short) of human insulin receptor β (see Uniprot #P06213; https://www.uniprot.org/uniprotkb/P06213/entry#sequences). “Tyr1150/1151” reflects the position of the phosphosites in isoform 2 when the 27 amino acid signal peptide is removed from the N-terminus.
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SignalStar Multiplex IHC Kits & Reagents have not yet been validated for use with antibodies outside of our menu. We currently offer custom conjugation of antibodies in our IHC-P-validated portfolio. In addition, SignalStar mIHC is a non-destructive technology that gives scientists the ability to perform other imaging assays on the same tissue, such as Hematoxylin and Eosin (H&E). If you are interested in learning more about our custom conjugation services for SignalStar mIHC, please use this link to contact our Sales Team.
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We do not observe any bias toward euchromatin or heterochromatin in our assays. In CUT&RUN, the target-specific antibody recruits the pAG-MNase and directs digestion to chromatin that is directly adjacent to the antibody, whether that be in euchromatin or heterochromatin. In this case, the active tethering of pAG-MNase to the chromatin allows for digestion to occur even in less accessible heterochromatin. Our CUT&RUN Assay Kit #86652 has been shown to work well with antibodies against active, accessible euchromatic histone modifications (see Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751) and activating transcription factors (see NF-kB p65 [D14E12] XP® Rabbit mAb #8242…
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Bio-Techne has tested various commercial buffers and common buffer components for compatibility with Simple Western™ and has found that some buffer components may negatively affect Simple Western™ results. For reference, Bio-Techne provides a helpful buffer compatibility chart on their website. At CST we use samples prepared in either SDS sample buffer (1X) (Blue Loading Buffer Pack #7722 or Red Loading Buffer Pack #7723) or Cell Lysis Buffer (1X) #9803. These buffers did not impact run performance in-house.