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Our validation of the Annexin V-FITC Early Apoptosis Detection Kit #6592 was performed using live cells in suspension, but the steps can be carried out on live adherent cells as well. If the option is available to scrape the cells from the growth surface and perform the staining in suspension, this would be the easiest way to follow the directions for use on the datasheet. Otherwise we suggest following the directions as closely as possible for adherent cells.
Cell states can be monitored using markers correlating to your process of interest. These assays and antibodies can be used to detect cell viability, senescence, proliferation, apoptosis, cytotoxicity and oxidative state.
Apoptosis is a type of programmed cell death that occurs normally throughout the lifespan and can also occur in response to harmful stimuli. Apoptotic cells are identified by their altered morphology, caspase activation, and the presence of damaged DNA.
Products and Related Resources for Cell Death and Viability SARS-CoV-2 Research
Cell viability is a measure of the proportion of live, healthy cells within a population. Assays to assess cell viability measure metabolic activity, ATP content, cell proliferation, or membrane integrity.
Cytotoxicity is the ability of an exogenous agent to damage or kill cells. Measuring cytotoxicity can help determine the effect of drugs on cell viability.
Apoptosis is a highly regulated form of programmed cell death that occurs in multicellular organisms throughout life and in response to cellular stress.
Learn more about how intracellular flow cytometry can be used to measure unique signaling events to do with apoptosis, pluripotency status and epigenetic activity.
Regulation of cell death due to viral infection is important for host survival. In this series, we look at pathways regulated through cellular responses to viruses.
Apoptosis is a regulated form of programmed cell death (PCD) that occurs without external influence.
Streamline your oncology therapeutic development with CST recombinant monoclonal antibodies, ELISA and cellular assay kits, custom products, and services.
Jak and cytokine receptor mutations found in various cancers, along with the corresponding PubMed reference(s).
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Western Blotting-Conformation, LC Specific, Anti-Biotin Secondary Ab: easy to follow directions describing the step by step experimental procedure.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
A scientific resource for the IQ protein domain containing information on structure, function, and domain binding to calmodulin.
An orthogonal antibody validation strategy is critical to verify existing validation data and identify any effects or artifacts that are directly related.
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
A scientific resource for the TPR protein domain containing information on structure, function, and domain binding in cell cycle-associated proteins.
An orthogonal strategy for antibody validation involves cross-referencing antibody-based results with data obtained using non-antibody-based methods.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.
A comprehensive list of peer-reviewed publications from Cell Signaling Technology.
Xenophagy provides an important defense against foreign pathogens such as bacteria and viruses by targeting them for degradation through autophagy.
Learn about antibody diversity, host species, isotypes, and how antibodies are developed for research, clinical diagnostics, and therapeutic uses.
Immunoprecipitation of Denatured Proteins Protocol: easy to follow directions describing the step by step experimental procedure.
