Resources results for "primers"
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We recommend using our CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415 to make DNA libraries for CUT&Tag. Our DNA Library Prep Kit for Illumina (ChIP-seq, CUT&Tag) #56795, and its paired Multiplex Oligos for Illumina (Dual Index Primers) (ChIP-seq, CUT&Tag) #47538 or Multiplex Oligos for Illumina (Single Index Primers) (ChIP-seq, CUT&Tag) #29580 are not compatible with CUT&Tag DNA samples.
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We recommend using our SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 for making DNA libraries with CUT&RUN or ChIP DNA. This kit should be paired with our SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers) #47538 or SimplechIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) #29580.
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Whether performing CUT&RUN with downstream qPCR or NG-seq analysis, we always recommend using a CUT&RUN-validated positive control antibody like our Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 and a negative control antibody like our Rabbit (DA1E) mAb IgG XP® Isotype Control Antibody #66362. These antibodies can be paired with our SimpleChIP® Human RPL30 Exon 3 Primers #7014 or SimpleChIP® Mouse RPL30 Intron 2 Primers #7015 to show that your CUT&RUN assay is working regardless of the performance of your desired target-specific antibody. All of these cont…