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Exactly what is ELISA? Learn about the enzyme-linked immunosorbent assay, the specific interaction between antibody & antigen. Click here.
Illustration of Motif Antibody Coverage
The accuracy of western blot results relies heavily on the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.
This post summarizes the findings in the Nature paper YAP-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer.
An outline and examples of the steps performed at Cell Signaling Technology to validate target specificity of an antibody for use in immunohistochemical analysis.
Learn how to visualize protein expression in tissue with immunohistochemistry (IHC). See examples of successfully optimized IHC assays.
Unravel the HER2 signaling pathway and its role in cell growth and cancer. Explore the molecular mechanisms and signaling events. Click here.
Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.
Catalog numbers in tabular format for control cell extracts and proteins with links to product pages.
CST Technical Support Article
The Jak/Stat Utilization Table displays the combinatorial use of tyrosine kinases and Stat proteins in cytokine/growth factor signaling.
Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.
Get the reagents and supplies for immunohistochemistry (IHC) protocols including antibodies, diluent, blocking buffer, chromogens, and controls
Expert-reviewed interactive pathway providing a current overview of ErbB/HER Signaling.
A Hallmark of Cancer, sustaining proliferative signaling is used by cancer cells to stimulate growth and relies on Akt, MAPK/Erk, and MTOR pathways.
Eliminate non-specific binding and high background with the immunohistochemitry (IHC) troubleshooting guide and positive/negative controls
Senescence is the irreversible arrest of proliferation in response to a variety of cellular stressors. This state is associated with changes in intracellular signaling pathways. Learn more.
Information and case study data for the PTMScan Direct Cell Cycle and DNA Damage Service offered by CST.
Case Study 4: Exceptional Performance of XP Monoclonal Antibodies from Cell Signaling Technology.
In response to a variety of environmental factors, cells may permanently cease proliferation and enter a state known as cellular senescence.
Immunohistochemistry is a method for studying antigen localization in tissue sections using antibodies. Learn sample preparation, labeling, & visualization.
Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Information and case study data for the PTMScan Direct PI3K/Akt Service offered by CST, which allows for targeted screening of 105 proteins within the Akt pathway.
Information and case study data for the PTMScan Direct Ser/Thr Kinases Service, which allows for the targeted screening of a defined set of protein modification sites.
Information and case study data for the PTMScan Direct Tyrosine Kinases Service from Cell Signaling Technology.
Kinase-disease associations table showing kinases, kinase groups, disease types, and the molecular biology basis for disease.
Hallmarks of Antibody Validation: Ranged Strategy. Binary models are not always readily available and can be time-consuming to produce for validating an antibody.