Resources results for "hif-1"

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Resources (1460)

Hypoxia Signaling

Learn here about the HIF-1 signaling pathway and its impact on oxygen homeostasis and cellular adaptation. Click here.

Hypoxia and Cancer – the role of HIF-1α in oxygen sensing, metabolism, and tumorigenesis

Molecular oxygen is an essential element for metazoan life. Among its many roles, it functions as the final electron acceptor (oxidizing agent) during oxidative phosphorylation, a metabolic chain-reaction that generates energy in the form of ATP. HIF-1 alpha

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It is very difficult to detect HIF-1alpha under normoxic conditions as the protein is quickly degraded. To see strong signal, it is necessary to induce the expression of the protein by either stressing the cells under hypoxic conditions or treating with a hydroxylase inhibitor such as cobalt chloride.

Tumor Angiogenesis

Angiogenesis, the formation of new blood vessels from pre-existing blood vessels, plays a key role in tumorigenesis.

How Do I Measure Metabolism?

Several different assays can be used by scientists to measure metabolism in a variety of contexts. These include methods to determine metabolic rates, key signaling pathways, and environmental cues.

Angiogenesis Resources

Overview of Angiogenesis antibodies & related reagents, interactive pathway diagrams, and other technical resources.

PAS Protein Domain

A scientific resource for the PAS protein domain containing information on structure, function, and domain binding.

Tools for Modulating Cells

Cell behavior is a tightly regulated process where various signals work together to elicit a specific response. Unraveling these mechanisms allows us to understand how cells are regulated in a normal state.

Warburg Effect

Expert-reviewed interactive pathway providing a current overview of the Warburg Effect.

VHL Protein Domain

A scientific resource for the VHL protein domain containing information on structure, function, and domain binding to hydroxylated proline residues.

Interrogating Immnunometabolism in the TME Using a Metabolic Checkpoint

Metabolic processes regulate immune cell function in a process known as immunometabolism, which can be studied using these macrophage and T-cell markers.

Hallmarks of Cancer: Inducing Angiogenesis Energetics

Cancer cells stimulate the growth of blood vessels to supply nutrients to tumors. Angiogenesis is the formation of new blood vessels from pre-existing blood vessels. This plays an important role in tumor growth.

Hypoxia and Its Role in Metabolism

Hypoxia causes cellular stress and alters normal metabolic activity. These pathways allow cells to survive and recover during bouts of low oxygen.

Overview of Metabolism and Metabolic Disorders

Metabolism defines all of the chemical changes or processes within cells, tissues, and organisms that sustain cellular homeostasis.

Ubiquitin Ligase Table

The Ubiquitin Ligase Table provides a comprehensive list of E3 ubiquitin ligases along with their substrates and corresponding PubMed reference(s).

Understanding Mitophagy

Mitophagy is a well-studied example of selective autophagy. This post explores mitophagy functions and the consequence of excessive or inadequate mitophagy

ChIP Validated Antibodies and Primer Pairs

SimpleChIP Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.

Matched Antibody Pairs

Find validated CST® matched antibody pairs and streamline the development of your High-Throughput ELISA-Like Immunoassays for screening.

Hallmarks of Cancer: Deregulating Cellular Energetics

Cancer cells need a lot of energy to grow fast—to do so, they show abnormal metabolic pathways.

1% for the Planet Member

CST is a proud member of the 1% for the Planet organization, giving annually to benefit social and environmental causes.

Cell Signaling Technology Joins 1% for the Planet

We have committed to donating 1% of our total annual revenue to environmental giving in collaboration with 1% for the Planet. Join the movement.

Cell Signaling Technology becomes 1% for the Planet Lighthouse Member

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We do not perform enzymatic activity assays internally using the Cell Lysis Buffer (10X) #9803, so we are unable to guarantee that the enzymatic activity will be conserved. We routinely use this buffer in immunoassays such as western immunoblot, IP, and ELISA without issue.

As a reference, the components of the Cell Lysis Buffer (10X) #9803 are provided below:
20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na₂EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na₃VO₄, 1 µg/ml leupeptin

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Our Calpain 1 Large Subunit (Mu-type) Antibody #2558 detects endogenous levels of total calpain 1 (large subunit) protein. The antibody detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28. The intensity of the cleaved band can vary depending on the species and type of cell lysate used.

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When we validate an antibody for Simple Western™ we titrate the antibody dilution against a broad range of lysate concentrations. We recommend testing at least three antibody dilutions (1:10, 1:50 and 1:250) across a range of lysate concentrations (0.1 mg/mL – 1 mg/mL).

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We typically recommend the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 that comes with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581 as it has been validated for use with the kit. The PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 was made specifically for this assay kit and for ELISA formats. We have not tested any other lysis buffers with the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581, so we cannot guarantee the performance. The ingredients of the PathScan® Sandwich ELISA Lysis Buffer (1X) #7018 are as follows: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 ug/ml leupeptin.

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CIP/Lambda Phosphatase Treatment of Cell Lysates:

1) Wash cells 2X with ice-cold PBS.

2) Add cold 1X cell lysis buffer that does not include phosphatase inhibitors [1-2 E7 cells per ml or 0.4ml per 10 cm plate (80-90% confluent)]. Our cell lysis buffer is prepared as follows:

20 mM Tris-HCl (pH 7.5)
150 mM NaCl
1 mM Na2EDTA
1 mM EGTA
1% Triton
1 ug/ml leupeptin
1mM PMSF

3) Add 20ul Quick CIP (NEB #M0525; https://www.neb.com/products/m0525-quick-cip#Product%20Information) per 400ul of cell extract.

4) Incubate with Quick CIP at 37C for 1 hour.

5) Add 20ul lambda protein phosphatase (CST

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The regions recognized by our PD-1 and PD-L1 antibodies are as follows:

PD-1 (EH33) Mouse mAb (IHC-Specific) #43248 binds the extracellular domain.
PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) #84651 binds the intracellular domain.
PD-1 (D4W2J) XP® Rabbit mAb #86163 binds the intracellular domain.
PD-L1 (E1L3N®) XP® Rabbit mAb #13684 binds the intracellular domain.
PD-L1 (Extracellular Domain Specific) (E1J2J) Rabbit mAb #15165 binds the extracellular domain.
PD-L1 (405.9A11) Mouse mAb #29122 binds the intracellular domain.
PD-L1 (D4H1Z) Rabbit mAb #60475 binds the extracellular domain.
PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) #64988 binds the intracellular domain.
PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb #86744 binds the extracellular domain.

SignalStar™ Multiplex Immunohistochemistry Assay for Use on BOND RX Fully Automated Research Stainer by Leica Biosystems

Exceptional Specificity of XP® Monoclonal Antibodies

Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.