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Unfortunately, we have not internally validated the use of acidic samples with our Cyclic AMP XP(R) Assay Kit #4339. We have had customers try this method, preparing their samples with 0.1M HCL and then neutralizing. The customers did not have success with this method.If using acidic sample preparation, we recommend further diluting samples in 1X PBS after neutralization, prior to adding samples to the plate. Again, as we have not internally validated this, we cannot guarantee this kit will work with acidic samples.
Drs. Benjamin Wolozin and Dorothee Dormann present recent research on phase separation mechanisms regulating stress granule proteins implicated in ALS, frontotemporal dementia, and other neurodegenerative diseases.
CST Technical Support Article
In our experience, digesting 4x10^6 cells yields 10-20 ug of chromatin, which is why this is mentioned in our ChIP FAQ. However, as stated in the protocols for our SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005, approximately 5ug - 10ug chromatin is required for ChIP depending on your target. In our experience, for histone (PTM) ChIP, as low as 1-2 ug of chromatin DNA is required per IP. For transcription factor ChIP, a minimum of 5-8 ug chromatin DNA is required per IP. For co-factor ChIP, a minimum of 10 ug chromatin is necessary per IP. This is why all of our validated antibodies have optimal dilutions based on 10ug per IP.
A comprehensive, step-by-step video of the CUT&RUN Protocol, from sample preparation to CUT&RUN DNA purification and qPCR quantification. Expert tips for critical steps and experimental design are highlighted to help you successfully complete the assay.
To enable surface characterization of chimeric antigen receptors (CARs), scientists at CST developed versatile and specific antibodies that detect the linker sequence of scFv domains. Watch the poster presentation from AACR.
Multiplex Oligos for Illumina Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.
The new PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit #59322 uses the same antibody as in the traditional PTMScan Ubiquitin Remnant Motif (K-ε-GG) Kit #5562 and PTMScan Pilot Ubiquitin Remnant Motif (K-ε-GG) Kit #14482. This means it has the same affinity for the K-e-GG remnant after trypsin cleavage.However, we have changed the antibody – bead linkage to be near-covalent in strength so the antibody does not contaminate the PTM peptides during the elution step. This prevents issues with the liquid chromatography column clogging and increases the yield of PTM peptides identified. The specificity is also improved with changes to the bind and wash buffers that help reduce non-specific peptide binding.In addition, the new HS kit beads are magnetic, rather than agarose, which simplifies the workflow by eliminating multiple centrifugation steps during the bead washing and elution steps.
Performance characteristics of XP rabbit monoclonal antibodies from Cell Signaling Technology.
Drs. Michael Keebler and Chaolin Zhang present work on protein-RNA dynamics in neuronal synapses and RNA splice regulation in neuronal development.
Keep your cell and tissue culture free of contamination - watch this how-to video for tips and advice from our scientists.