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A scientific resource for the MIU protein domain containing information on structure, function, and domain binding to ubiquitin during vesicle trafficking.
We provide example data for our PTMScan enrichment kits on this webpage:https://www.cellsignal.com/applications/proteomics/ptmscan-motif-antibody-kitsTo the right of each product listing are two hypertext links to the data: an Excel file of the informatic search results and the RAW files or primary mass spectrometry data. Each PTMScan enrichment is run as technical replicates producing two RAW files, the Excel file contains the combined results of these two RAW files.The Excel file contains the processed informatics of all the identified PTM peptides. The Excel file also contains information on the location of the modified site, the NCBI accession number of the parent protein and isoforms, whether the site has been reported in the scientific literature, and detailed metrics of the quality of the identified peptide.Two additional tabs on each Excel file contain information on the methods used for mass spectrometry, and a glossary of terms.
Senescent cells increase in number during aging and have been implicated in the decline of organismal function over time, as well as in the progression of age-related diseases.
Lymphoid lineage cells can be characterized using antibodies for cell type-specific markers, allowing cells to be visualized using flow cytometry or IHC.
The average diameter of each Concanavalin A bead particle is ~1.0µm while the size range of a cell is 0.1-100 µm (the most commonly used cell lines like HeLa, HepG2 and HEK293 all have a cell size > 10µm). Therefore, in most cases, a cell is coated by multiple beads. This has also been confirmed by inspection under a microscope.
A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.
Do ChIP-validated antibodies work in CUT&RUN assays? Not necessarily. We found only 50-60% of ChIP- or ChIP-seq antibodies were compatible with CUT&RUN.
This webinar explores the impact of senescence on age-related dysfunction and chronic disease and introduces potential therapies targeting senescent cells.
In section C, part 8 of the Senescence β-Galactosidase Staining Kit #9860 protocol, you may overlay the plates with 70% glycerol directly after removing the staining solution. It is not necessary to wash off the staining solution prior to overlaying with glycerol.
Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.
Lymphoma is a cancer of the lymphatic system, a key part of the body’s immune system. Treatment options & patient prognoses differ based on lymphoma type.
Our Tau (D1M9X) XP® Rabbit mAb #46687 is predicted to recognize 2N4R (tau40), 2N3R (tau39), 1N4R (tau46), 1N3R (tau37), 0N4R (tau24), and 0N3R (tau23), as all six isoforms include the antigenic peptide.
A scientific resource for the LRR protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.
Due to the nature of the Cell Fractionation Kit #9038 and the localization of each of the proteins in the Loading Control Sampler Kit #5142, the proteins will not be equally expressed in all of the fractions. We have not found a target that shows equal expression across all the cytoplasmic and membrane fractions. The whole cell lysate (WCL) can be used as a reference to compare against each fraction. Efficiency of separation can be measured using the antibodies in the Cell Fractionation Antibody Sampler Kit #11843.
Antibody 5G10, which targets S6 ribosomal proteins, can be used as a control for formaldehyde fixation conditions and sample quality in IF experiments.
Our BSA #9998 is tested for protease activity and each lot can contain up to 0.005 units/mg. However it is not tested for globulins, DNase, RNase or endonuclease activity. Therefore, we are unable to say whether or not the product actually contains them or at what percentages.
New antibodies for Alzheimer's research were presented at SfN 2022, including tools to study neuroinflammation in glial cells like microglia and astrocytes
Depending on the target, the FastScan™ ELISA Cell Extraction Enhancer Solution #25243 usually increases the sensitivity/signal and performance of the kit. Therefore, if it is omitted, your signal may be lower, however the extent of this effect will vary from kit to kit. We recommend using the enhancer to avoid this possibility. If you forgot to add the enhancer to the FastScan™ ELISA Cell Extraction Buffer #69905 that was used to make the sample lysate, you can spike in the 50x Enhancer solution afterwards (and always add the enhancer to the extraction buffer that is used to dilute the samples). We believe the enhancer increases the signal by helping increase the recognition and binding of the target protein by the antibodies in the kit.
Antibodies, and proteins in general, are less susceptible to degradation when stored at higher concentrations (i.e., greater than or equal to 1.0 mg/ml). Therefore, because our off-the-shelf antibodies are offered over a wide range of concentrations, BSA is included in the storage buffer as a stabilizer.
A scientific resource for the HECT protein domain containing information on structure, function, and domain binding in ubiquitin-mediated protein degradation.
A scientific resource for the GAT protein domain containing information on structure, function, and domain binding to ubiquitin and GTP-bound ARF.
Much has been written about the hallmarks of cancer, but we expand on the story with The Guide to the Hallmarks of Cancer Research Targets.
Our Cell Fractionation Kit #9038 provides a fast and efficient way of separating cultured cells into three distinct fractions for analysis by SDS-PAGE and western blotting. We have not tested fractionated samples using mass spectrometry in-house and it is difficult to speculate how well they would work. The buffer contains SDS so you may need to use a commercially available SDS purification kit if you would like to prepare the fractionated samples for mass spectrometry by in-solution digestion; in-gel digestion would account for SDS removal in the extraction already. We cannot guarantee activity by mass spectrometry as it is not the primary intended use of this kit.
A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.
The “classic” PTMScan® kits that use agarose beads and the PTMScan HS kits that use magnetic beads are both designed for a single PTM peptide enrichment. The nature of the acidic elution step, which leads to the release of bound PTM peptides, denatures the antibodies. This denaturing step alters the conformation of the antibodies. Therefore, employing these antibodies for subsequent enrichments may produce inconsistent results in PTM peptide enrichment.
Due to the fact that the total α-Adducin (D7T7R) Rabbit mAb #70174 does not recognize this band in our in-house testing, the 150kDa background band seen with our Phospho-α-Adducin (Ser12) (E5X8Y) Rabbit mAb #84214 is likely an unrelated protein induced by nocodazole.
A scientific resource for the DH protein domain containing information on structure, function, and domain binding during Rho GTPase activation.
This webinar will highlight how antibody specificity affects reproducibility in biomedical research and will introduce viewers to the key requirements for proper antibody validation.
Sometimes understanding complex signaling is overwhelming. Try thinking about it as the wiring on a construction site. Maybe that will help!