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Many types of ELISA have been developed to address different research needs from direct, indirect, to sandwich, competitive and multiplex. Learn here
This webinar will focus on the importance of the immune regulatory context of the TME
FastScan Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
The ELISA method has many variations, all of which rely on complex of an antigen and an antibody/enzyme conjugate. Signal is generated by turnover of substrate.
Expert-reviewed interactive pathway providing a current overview of angiogenesis pathways and links to products by Cell Signaling Technology.
Click here to discover the intricacies of methylation pathways and their role in gene regulation and epigenetic modifications.
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
PathScan ELISA kits detect endogenous levels of key signaling proteins using a traditional sandwich-based assay format in phospho-specific or total protein options.
This webinar will focus on the potential role of innate immunity in neurodegeneration and cognitive function, particularly in Alzheimer's disease.
Expert-reviewed interactive pathway providing a current overview of the Inflammasome Signaling Pathway.
Interact with this diagram showing the epigenetic writers and erasers of histone H2A, histone H2B, and Histone H4, including the involvement of acetyltransferases, deacetylases, methyltransferases, demethylases and kinases.
We have performed CUT&RUN on dozens of cultured adherent and suspension cell lines, both human and mouse. So far we have not noticed any significant difference in performance between these cell lines.
The Ser485 site detected by our Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 has been identified as an inhibitory phosphosite of APMKα1 and will be phosphorylated to a lesser degree when AMPK is activated in response to nutrient-deprived conditions (e.g., serum or glucose starvation). Therefore, you should see a decrease in signal intensity with #2537 in serum-starved samples, such as the positive extract included in our AMPK Control Cell Extracts #9158.
Achieve high-quality immunohistochemistry staining with our specific antibodies & optimized protocols. Learn more about our IHC staining solutions.
CST is committed to following the Paris Climate Agreement.
CST promotes alternative transportation through a variety of programs.
For PTMScan experiments, the stage tip, or small-scale peptide desalting is a critical part of the experiment for a number of reasons. Detailed instructions for performing stage tip peptide desalting are included at the end of each PTMScan kit protocol.The stage tip step will remove salts and other contaminants prior to mass spectrometry that can distract the mass spectrometer from peptide identification. Importantly, this step will also serve to reduce the amount of antibody that may co-elute with the peptides and contaminate the analytical column or LC. This will improve the longevity of the analytical column and lead to more reproducible data.Stage tips can be made using an 18 gauge needle and C-18 disks, according to the method published by Rappsilber et al (Anal. Chem. 2003, 75, 3, 663–670, 2002). Alternatively, the tips can be purchased from a number of suppliers.The key to a successful desalting step is using a high-capacity stage tip, or one that is suitable for ~10ug of…
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Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat
CST offers flow validated antibodies for single cell mass cytometry (MC) and IHC validated antibodies for imaging mass cytometry (IMC)